Recombinant human Fyn protein is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, WB, FuncS.
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Publications
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- Nature communications 14:62082023Acetylation-dependent coupling between G6PD activity and apoptotic signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFang Wu,Natali H Muskat,Inbar Dvilansky,Omri Koren,Anat Shahar,Roi Gazit,Natalie Elia,Eyal ArbelyPubMed 37798264
Key facts
- Purity
- >90% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
Amino acid sequence
M S P I L G Y W K I K G L V Q P T R L L L E Y L E E K Y E E H L Y E R D E G D K W R N K K F E L G L E F P N L P Y Y I D G D V K L T Q S M A I I R Y I A D K H N M L G G C P K E R A E I S M L E G A V L D I R Y G V S R I A Y S K D F E T L K V D F L S K L P E M L K M F E D R L C H K T Y L N G D H V T H P D F M L Y D A L D V V L Y M D P M C L D A F P K L V C F K K R I E A I P Q I D K Y L K S S K Y I A W P L Q G W Q A T F G G G D H P P K S D L V P R G S M G C V Q C K D K E A T K L T E E R D G S L N Q S S G Y R Y G T D P T P Q H Y P S F G V T S I P N Y N N F H A A G G Q G L T V F G G V N S S S H T G T L R T R G G T G V T L F V A L Y D Y E A R T E D D L S F H K G E K F Q I L N S S E G D W W E A R S L T T G E T G Y I P S N Y V A P V D S I Q A E E W Y F G K L G R K D A E R Q L L S F G N P R G T F L I R E S E T T K G A Y S L S I R D W D D M K G D H V K H Y K I R K L D N G G Y Y I T T R A Q F E T L Q Q L V Q H Y S E R A A G L C C R L V V P C H K G M P R L T D L S V K T K D V W E I P R E S L Q L I K R L G N G Q F G E V W M G T W N G N T K V A I K T L K P G T M S P E S F L E E A Q I M K K L K H D K L V Q L Y A V V S E E P I Y I V T E Y M N K G S L L D F L K D G E G R A L K L P N L V D M A A Q V A A G M A Y I E R M N Y I H R D L R S A N I L V G N G L I C K I A D F G L A R L I E D N E Y T A R Q G A K F P I K W T A P E A A L Y G R F T I K S D V W S F G I L L T E L V T K G R V P Y P G M N N R E V L E Q V E R G Y R M P C P Q D C P I S L H E L M I H C W K K D P E E R P T F E Y L Q S F L E D Y F T A T E P Q Y Q P G E N L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab84696 can be used as a WB positive control in conjunction with Anti-Fyn antibody [FYN-01] ab1881. |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance (PubMed:11536198, PubMed:15489916, PubMed:15557120, PubMed:16387660, PubMed:20100835, PubMed:7568038, PubMed:7822789). Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain (PubMed:15489916). Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions (PubMed:15489916). Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin) (PubMed:17194753). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT (PubMed:14707117, PubMed:15536091). Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage (PubMed:16841086). Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL1 and TRPC6 (PubMed:14761972, PubMed:18258597, PubMed:19179337). Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein (PubMed:11162638, PubMed:12788081, PubMed:19652227). Involved in reelin signaling by mediating phosphorylation of DAB1 following reelin (RELN)-binding to its receptor (By similarity). Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation (PubMed:22080863). Phosphorylates PTK2B/PYK2 in response to T-cell receptor activation (PubMed:20028775). Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts (PubMed:18056706). CSK maintains LCK and FYN in an inactive form (By similarity). Promotes CD28-induced phosphorylation of VAV1 (PubMed:11005864). In mast cells, phosphorylates CLNK after activation of immunoglobulin epsilon receptor signaling (By similarity). Can also promote CD244-mediated NK cell activation (PubMed:15713798).
Alternative names
Tyrosine-protein kinase Fyn, Proto-oncogene Syn, Proto-oncogene c-Fyn, Src-like kinase, p59-Fyn, SLK, FYN
Recommended products
Recombinant human Fyn protein is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, WB, FuncS.
Key facts
- Purity
- >90% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
- Biological activity
- Specific activity: 166nmol/min/mg.
- Accession
- P06241-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF
Sequence info
Amino acid sequence
- M S P I L G Y W K I K G L V Q P T R L L L E Y L E E K Y E E H L Y E R D E G D K W R N K K F E L G L E F P N L P Y Y I D G D V K L T Q S M A I I R Y I A D K H N M L G G C P K E R A E I S M L E G A V L D I R Y G V S R I A Y S K D F E T L K V D F L S K L P E M L K M F E D R L C H K T Y L N G D H V T H P D F M L Y D A L D V V L Y M D P M C L D A F P K L V C F K K R I E A I P Q I D K Y L K S S K Y I A W P L Q G W Q A T F G G G D H P P K S D L V P R G S M G C V Q C K D K E A T K L T E E R D G S L N Q S S G Y R Y G T D P T P Q H Y P S F G V T S I P N Y N N F H A A G G Q G L T V F G G V N S S S H T G T L R T R G G T G V T L F V A L Y D Y E A R T E D D L S F H K G E K F Q I L N S S E G D W W E A R S L T T G E T G Y I P S N Y V A P V D S I Q A E E W Y F G K L G R K D A E R Q L L S F G N P R G T F L I R E S E T T K G A Y S L S I R D W D D M K G D H V K H Y K I R K L D N G G Y Y I T T R A Q F E T L Q Q L V Q H Y S E R A A G L C C R L V V P C H K G M P R L T D L S V K T K D V W E I P R E S L Q L I K R L G N G Q F G E V W M G T W N G N T K V A I K T L K P G T M S P E S F L E E A Q I M K K L K H D K L V Q L Y A V V S E E P I Y I V T E Y M N K G S L L D F L K D G E G R A L K L P N L V D M A A Q V A A G M A Y I E R M N Y I H R D L R S A N I L V G N G L I C K I A D F G L A R L I E D N E Y T A R Q G A K F P I K W T A P E A A L Y G R F T I K S D V W S F G I L L T E L V T K G R V P Y P G M N N R E V L E Q V E R G Y R M P C P Q D C P I S L H E L M I H C W K K D P E E R P T F E Y L Q S F L E D Y F T A T E P Q Y Q P G E N L
- Accession
- P06241
- Protein length
- Full Length
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance (PubMed:11536198, PubMed:15489916, PubMed:15557120, PubMed:16387660, PubMed:20100835, PubMed:7568038, PubMed:7822789). Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain (PubMed:15489916). Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions (PubMed:15489916). Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin) (PubMed:17194753). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT (PubMed:14707117, PubMed:15536091). Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage (PubMed:16841086). Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL1 and TRPC6 (PubMed:14761972, PubMed:18258597, PubMed:19179337). Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein (PubMed:11162638, PubMed:12788081, PubMed:19652227). Involved in reelin signaling by mediating phosphorylation of DAB1 following reelin (RELN)-binding to its receptor (By similarity). Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation (PubMed:22080863). Phosphorylates PTK2B/PYK2 in response to T-cell receptor activation (PubMed:20028775). Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts (PubMed:18056706). CSK maintains LCK and FYN in an inactive form (By similarity). Promotes CD28-induced phosphorylation of VAV1 (PubMed:11005864). In mast cells, phosphorylates CLNK after activation of immunoglobulin epsilon receptor signaling (By similarity). Can also promote CD244-mediated NK cell activation (PubMed:15713798).
- Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
- Post-translational modifications
Autophosphorylated at Tyr-420 (By similarity). Phosphorylation on the C-terminal tail at Tyr-531 by CSK maintains the enzyme in an inactive state (PubMed:1699196). PTPRC/CD45 dephosphorylates Tyr-531 leading to activation (PubMed:1533589). Ultraviolet B (UVB) strongly increase phosphorylation at Thr-12 and kinase activity, and promotes translocation from the cytoplasm to the nucleus (PubMed:15537652). Dephosphorylation at Tyr-420 by PTPN2 negatively regulates T-cell receptor signaling (PubMed:22080863). Phosphorylated at tyrosine residues, which can be enhanced by NTN1 (By similarity).
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Active Poly (Glu, Tyr) peptide ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Fyn is a Src family tyrosine kinase with a molecular weight of approximately 59 kDa. It is often referred to as Fyn kinase and plays a significant role in signal transduction processes. This protein is observed in various tissues with high expression in the brain T cells and platelets. Fyn kinase is known for its function in cell communication and regulates different cellular processes in these tissues.
- Biological function summary
Fyn kinase influences cell growth differentiation and survival. It is a part of larger signaling complexes including those accountable for the development and function of the central nervous system. The protein's activity is important for neural plasticity and supports synaptic communication particularly within the brain. Fyn can interact with other proteins in these complexes to help direct cellular responses to external signals.
- Pathways
Fyn kinase is central to several intracellular signaling pathways such as the T cell receptor (TCR) signaling pathway and the integrin signaling pathway. The TCR signaling pathway involves the regulation of immune responses while the integrin signaling pathway contributes to cell adhesion and migration. Other proteins like Lck and Yes interact with Fyn within these pathways adding another layer of regulation and response in various cell types.
- Associated diseases and disorders
Fyn kinase has associations with Alzheimer's disease and certain types of cancer. In Alzheimer's dysregulation of Fyn activity contributes to synaptic dysfunction and neuronal damage. In cancer aberrant Fyn signaling can affect cellular proliferation and metastasis. Proteins such as Tau in Alzheimer's disease and Src in cancer relate to Fyn through their respective pathological processes.
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6 product images
Western blot - Recombinant human Fyn protein (ab84696)
All lanes: Western blot - Anti-Fyn antibody [FYN-01] (Anti-Fyn antibody [FYN-01] ab1881) at 1 µg/mL
All lanes: Western blot - Recombinant human Fyn protein (ab84696) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 60 kDa
Exposure time: 10s
Functional Studies - Recombinant human Fyn protein (ab84696)
The specific activity of Fyn (ab84696) was determined to be 95 nmol/min/mg as per activity assay protocol and was equivalent to 141 nmol/min/mg as per radiometric assay
SDS-PAGE - Recombinant human Fyn protein (ab84696)
SDS PAGE analysis of ab84696
Functional Studies - Recombinant human Fyn protein (ab84696)
SDS-PAGE - Recombinant human Fyn protein (ab84696)
SDS Page analysis of ab84696
Western blot - Recombinant human Fyn protein (ab84696)
All lanes: Western blot - Anti-Fyn antibody [FYN-01] (Anti-Fyn antibody [FYN-01] ab1881) at 1 µg/mL
All lanes: Western blot - Recombinant human Fyn protein (ab84696) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 60 kDa
Exposure time: 10s
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