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Recombinant human Fyn protein is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, WB, FuncS.
Alternative names=Tyrosine-protein kinase Fyn, Proto-oncogene Syn, Proto-oncogene c-Fyn, Src-like kinase, p59-Fyn, SLK, FYN
- Purity
>90% Densitometry
- Expression system
Baculovirus infected Sf9 cells
- Tags
GST tag N-Terminus
- Applications
SDS-PAGE, WB, FuncS
- Biologically active
Yes
- Sequence
M S P I L G Y W K I K G L V Q P T R L L L E Y L E E K Y E E H L Y E R D E G D K W R N K K F E L G L E F P N L P Y Y I D
G D V K L T Q S M A I I R Y I A D K H N M L G G C P K E R A E I S M L E G A V L D I R Y G V S R I A Y S K D F E T L K V
D F L S K L P E M L K M F E D R L C H K T Y L N G D H V T H P D F M L Y D A L D V V L Y M D P M C L D A F P K L V C F K
K R I E A I P Q I D K Y L K S S K Y I A W P L Q G W Q A T F G G G D H P P K S D L V P R G S M G C V Q C K D K E A T K L
T E E R D G S L N Q S S G Y R Y G T D P T P Q H Y P S F G V T S I P N Y N N F H A A G G Q G L T V F G G V N S S S H T G
T L R T R G G T G V T L F V A L Y D Y E A R T E D D L S F H K G E K F Q I L N S S E G D W W E A R S L T T G E T G Y I P
S N Y V A P V D S I Q A E E W Y F G K L G R K D A E R Q L L S F G N P R G T F L I R E S E T T K G A Y S L S I R D W D D
M K G D H V K H Y K I R K L D N G G Y Y I T T R A Q F E T L Q Q L V Q H Y S E R A A G L C C R L V V P C H K G M P R L T
D L S V K T K D V W E I P R E S L Q L I K R L G N G Q F G E V W M G T W N G N T K V A I K T L K P G T M S P E S F L E E
A Q I M K K L K H D K L V Q L Y A V V S E E P I Y I V T E Y M N K G S L L D F L K D G E G R A L K L P N L V D M A A Q V
A A G M A Y I E R M N Y I H R D L R S A N I L V G N G L I C K I A D F G L A R L I E D N E Y T A R Q G A K F P I K W T A
P E A A L Y G R F T I K S D V W S F G I L L T E L V T K G R V P Y P G M N N R E V L E Q V E R G Y R M P C P Q D C P I S
L H E L M I H C W K K D P E E R P T F E Y L Q S F L E D Y F T A T E P Q Y Q P G E N L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes Ab84696 can be used as a WB positive control in conjunction with ab1881. |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Recombinant human Fyn protein is a Human Full Length protein, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, WB, FuncS.
Alternative names=Tyrosine-protein kinase Fyn, Proto-oncogene Syn, Proto-oncogene c-Fyn, Src-like kinase, p59-Fyn, SLK, FYN
Key facts
- Purity
>90% Densitometry
- Expression system
Baculovirus infected Sf9 cells
- Tags
GST tag N-Terminus
- Applications
SDS-PAGE, WB, FuncS
- Biologically active
Yes
- Biological activity
Specific activity: 166nmol/min/mg.
- Sequence
M S P I L G Y W K I K G L V Q P T R L L L E Y L E E K Y E E H L Y E R D E G D K W R N K K F E L G L E F P N L P Y Y I D
G D V K L T Q S M A I I R Y I A D K H N M L G G C P K E R A E I S M L E G A V L D I R Y G V S R I A Y S K D F E T L K V
D F L S K L P E M L K M F E D R L C H K T Y L N G D H V T H P D F M L Y D A L D V V L Y M D P M C L D A F P K L V C F K
K R I E A I P Q I D K Y L K S S K Y I A W P L Q G W Q A T F G G G D H P P K S D L V P R G S M G C V Q C K D K E A T K L
T E E R D G S L N Q S S G Y R Y G T D P T P Q H Y P S F G V T S I P N Y N N F H A A G G Q G L T V F G G V N S S S H T G
T L R T R G G T G V T L F V A L Y D Y E A R T E D D L S F H K G E K F Q I L N S S E G D W W E A R S L T T G E T G Y I P
S N Y V A P V D S I Q A E E W Y F G K L G R K D A E R Q L L S F G N P R G T F L I R E S E T T K G A Y S L S I R D W D D
M K G D H V K H Y K I R K L D N G G Y Y I T T R A Q F E T L Q Q L V Q H Y S E R A A G L C C R L V V P C H K G M P R L T
D L S V K T K D V W E I P R E S L Q L I K R L G N G Q F G E V W M G T W N G N T K V A I K T L K P G T M S P E S F L E E
A Q I M K K L K H D K L V Q L Y A V V S E E P I Y I V T E Y M N K G S L L D F L K D G E G R A L K L P N L V D M A A Q V
A A G M A Y I E R M N Y I H R D L R S A N I L V G N G L I C K I A D F G L A R L I E D N E Y T A R Q G A K F P I K W T A
P E A A L Y G R F T I K S D V W S F G I L L T E L V T K G R V P Y P G M N N R E V L E Q V E R G Y R M P C P Q D C P I S
L H E L M I H C W K K D P E E R P T F E Y L Q S F L E D Y F T A T E P Q Y Q P G E N L- Accession
- P06241-1
- Protein length
Full Length
- Animal free
No
- Nature
Recombinant
- Species
Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF
Specifications
- Form
Liquid
- Additional notes
Affinity purified.
General info
- Function
Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL1 and TRPC6. Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein. Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation. Phosphorylates PTK2B/PYK2 in response to T-cell receptor activation. Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts. CSK maintains LCK and FYN in an inactive form. Promotes CD28-induced phosphorylation of VAV1. In mast cells, phosphorylates CLNK after activation of immunoglobulin epsilon receptor signaling (By similarity).
- Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
- Post-translational modifications
Autophosphorylated at Tyr-420 (By similarity). Phosphorylation on the C-terminal tail at Tyr-531 by CSK maintains the enzyme in an inactive state (PubMed:1699196). PTPRC/CD45 dephosphorylates Tyr-531 leading to activation (PubMed:1533589). Ultraviolet B (UVB) strongly increase phosphorylation at Thr-12 and kinase activity, and promotes translocation from the cytoplasm to the nucleus (PubMed:15537652). Dephosphorylation at Tyr-420 by PTPN2 negatively regulates T-cell receptor signaling (PubMed:22080863). Phosphorylated at tyrosine residues, which can be enhanced by NTN1 (By similarity).
- Subcellular localisation
Nucleus
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity
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