Recombinant human G-CSF protein is a Human Fragment protein, in the 31 to 204 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
T P L G P A S S L P Q S F L L K C L E Q V R K I Q G D G A A L Q E K L C A T Y K L C H P E E L V L L G H S L G I P W A P L S S C P S Q A L Q L A G C L S Q L H S G L F L Y Q G L L Q A L E G I S P E L G P T L D T L Q L D V A D F A T T I W Q Q M E E L G M A P A L Q P T Q G A M P A F A S A F Q R R A G G V L V A S H L Q S F L E V S Y R V L R H L A Q P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes DTT-reduced protein migrates as 18 kDa protein due to glycosylation |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
Alternative names
C17orf33, GCSF, CSF3, Granulocyte colony-stimulating factor, G-CSF, Pluripoietin
Recommended products
Recombinant human G-CSF protein is a Human Fragment protein, in the 31 to 204 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- The bio-activity was determined by dose-dependent stimulation of the proliferation of M-NFS-60 cells. ED50<0.05ng/ml, corresponding to a specific activity of >2X107 Unit/mg.
- Accession
- P09919-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute the lyophilized protein in sterile deionized water to a final concentration of 200 ug/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 90% PBS, 10% Trehalose
Sequence info
Amino acid sequence
- T P L G P A S S L P Q S F L L K C L E Q V R K I Q G D G A A L Q E K L C A T Y K L C H P E E L V L L G H S L G I P W A P L S S C P S Q A L Q L A G C L S Q L H S G L F L Y Q G L L Q A L E G I S P E L G P T L D T L Q L D V A D F A T T I W Q Q M E E L G M A P A L Q P T Q G A M P A F A S A F Q R R A G G V L V A S H L Q S F L E V S Y R V L R H L A Q P
- Accession
- P09919
- Protein length
- Fragment
- Predicted molecular weight
- 18.7 kDa
- Amino acids
- 31 to 204
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
Purified by ion exchnage chromatography.
General info
- Function
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
- Sequence similarities
Belongs to the IL-6 superfamily.
- Post-translational modifications
O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Functional Studies - Recombinant human G-CSF protein (ab167700)
ab167700 at 2 μg/mL (100 μL/well) can bind Biotinylated Human G-CSF R, His Tag, Avi Tag (ab246045) with a linear range of 0.156-1.25 μg/mL.
SDS-PAGE - Recombinant human G-CSF protein (ab167700)
SDS PAGE analysis of ab167700 under reducing conditions. Stained overnight with Coomassie Blue.
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