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Recombinant human G-CSF protein is a Human Fragment protein, in the 31 to 204 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Alternative names=Granulocyte colony-stimulating factor, G-CSF, Pluripoietin, GCSF, C17orf33, CSF3
- Purity
>95% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
- Sequence
T P L G P A S S L P Q S F L L K C L E Q V R K I Q G D G A A L Q E K L C A T Y K L C H P E E L V L L G H S L G I P W A P L S S C P S Q A L Q L A G C L S Q L H S G L F L Y Q G L L Q A L E G I S P E L G P T L D T L Q L D V A D F A T T I W Q Q M E E L G M A P A L Q P T Q G A M P A F A S A F Q R R A G G V L V A S H L Q S F L E V S Y R V L R H L A Q P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes DTT-reduced protein migrates as 18 kDa protein due to glycosylation |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Recombinant human G-CSF protein is a Human Fragment protein, in the 31 to 204 aa range, expressed in HEK 293, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Alternative names=Granulocyte colony-stimulating factor, G-CSF, Pluripoietin, GCSF, C17orf33, CSF3
Key facts
- Purity
>95% SDS-PAGE
- Endotoxin level
< 1 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE, FuncS
- Biologically active
Yes
- Biological activity
The bio-activity was determined by dose-dependent stimulation of the proliferation of M-NFS-60 cells. ED50<0.05ng/ml, corresponding to a specific activity of >2X107 Unit/mg.
- Predicted molecular weight
18.7 kDa
- Amino acids
31 to 204
- Sequence
T P L G P A S S L P Q S F L L K C L E Q V R K I Q G D G A A L Q E K L C A T Y K L C H P E E L V L L G H S L G I P W A P L S S C P S Q A L Q L A G C L S Q L H S G L F L Y Q G L L Q A L E G I S P E L G P T L D T L Q L D V A D F A T T I W Q Q M E E L G M A P A L Q P T Q G A M P A F A S A F Q R R A G G V L V A S H L Q S F L E V S Y R V L R H L A Q P
- Accession
- P09919-1
- Protein length
Fragment
- Animal free
No
- Nature
Recombinant
- Species
Human
- Reconstitution
Reconstitute the lyophilized protein in sterile deionized water to a final concentration of 200 ug/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 90% PBS, 10% Trehalose
Specifications
- Form
Lyophilized
- Additional notes
Purified by ion exchnage chromatography.
General info
- Function
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
- Sequence similarities
Belongs to the IL-6 superfamily.
- Post-translational modifications
O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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