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AB9692

Recombinant human G-CSF protein

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Recombinant human G-CSF protein is a Human Full Length protein, expressed in Escherichia coli, with >95%, < 1 EU/µg endotoxin level, suitable for FuncS, SDS-PAGE.

View Alternative Names

C17orf33, GCSF, CSF3, Granulocyte colony-stimulating factor, G-CSF, Pluripoietin

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

The ED50, determined by the dose-dependent stimulation of the proliferation of murine NSF-60 cells, is < 0.1 ng/ml, corresponding to a specific activity of > 1 x 107 units/mg.

Accession

P09919

Animal free

No

Carrier free

No

Species

Human

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Primary Antibodies

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Anti-G-CSF antibody [EPR3203(N)(B)]

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>This protein can be used with studies using <a href='/en-us/products/primary-antibodies/g-csf-antibody-ab9691'>ab9691</a> and <a href='/en-us/products/unavailable/gcsf-receptor-antibody-s-1268-ab18627'>ab18627</a>.</p>" } } }
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Product details

The ED50 as determined by the dose-dependent stimulation of the proliferation of murine NFS-60 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.
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Sequence info

[{"sequence":"Human G-CSF is a 18.7 kDa protein containing 174 amino acid residues:\r\n\r\nTPLGPASSLP \tQSFLLKCLEQ\tVRKIQGDGAA\tLQEKLCATYK\tLCHPEELVLL\tGHSLGIPWAPLSSCPSQALQ\tLAGCLSQLHS\tGLFLYQGLLQ\tALEGISPELG\tPTLDTLQLDV\tADFATTIWQQMEELGMAPAL\tQPTQGAMPAF\tASAFQRRAGG\tVLVASHLQSF\tLEVSYRVLRH\tLAQP","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P09919","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Lyophilized

Additional notes

Sterile filtered Greater than 98% pure by SDS-PAGE and HPLC analyses.

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General info

Function

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.

Sequence similarities

Belongs to the IL-6 superfamily.

Post-translational modifications

O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).

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Product protocols

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Target data

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
See full target information CSF3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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