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Recombinant Human G3BP protein is a Human Full Length protein, in the 1 to 466 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.

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Images

SDS-PAGE - Recombinant Human G3BP protein (AB103304), expandable thumbnail

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag C-Terminus

Applications

SDS-PAGE, MS

Biologically active

No

Amino acid sequence

M V M E K P S P L L V G R E F V R Q Y Y T L L N Q A P D M L H R F Y G K N S S Y V H G G L D S N G K P A D A V Y G Q K E I H R K V M S Q N F T N C H T K I R H V D A H A T L N D G V V V Q V M G L L S N N N Q A L R R F M Q T F V L A P E G S V A N K F Y V H N D I F R Y Q D E V F G G F V T E P Q E E S E E E V E E P E E R Q Q T P E V V P D D S G T F Y D Q A V V S N D M E E H L E E P V A E P E P D P E P E P E Q E P V S E I Q E E K P E P V L E E T A P E D A Q K S S S P A P A D I A Q T V Q E D L R T F S W A S V T S K N L P P S G A V P V T G I P P H V V K V P A S Q P R P E S K P E S Q I P P Q R P Q R D Q R V R E Q R I N I P P Q R G P R P I R E A G E Q G D I E P R R M V R H P D S H Q L F I G N L P H E V D K S E L K D F F Q S Y G N V V E L R I N S G G K L P N F G F V V F D D S E P V Q K V L S N R P I M F R G E V R L N V E E K K T R A A R E G D R R D N R L R G P G G P R G G L G G G M R G P P R G G M V Q K P G F G V G R G L A P R Q V E H H H H H H

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

MS

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Protein involved in various processes, such as stress granule formation and innate immunity (PubMed:12642610, PubMed:20180778, PubMed:23279204, PubMed:30510222, PubMed:30804210). Plays an essential role in stress granule formation (PubMed:12642610, PubMed:20180778, PubMed:23279204, PubMed:32302570, PubMed:32302571, PubMed:32302572, PubMed:34739333, PubMed:35977029, PubMed:36183834, PubMed:36279435, PubMed:36692217, PubMed:37379838). Stress granules are membraneless compartments that store mRNAs and proteins, such as stalled translation pre-initiation complexes, in response to stress (PubMed:12642610, PubMed:20180778, PubMed:23279204, PubMed:27022092, PubMed:32302570, PubMed:32302571, PubMed:32302572, PubMed:36279435, PubMed:37379838). Promotes formation of stress granules phase-separated membraneless compartment by undergoing liquid-liquid phase separation (LLPS) upon unfolded RNA-binding: functions as a molecular switch that triggers RNA-dependent LLPS in response to a rise in intracellular free RNA concentrations (PubMed:32302570, PubMed:32302571, PubMed:32302572, PubMed:34739333, PubMed:36279435, PubMed:36692217). Also acts as an ATP- and magnesium-dependent helicase: unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency (PubMed:9889278). Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA (PubMed:9889278). Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends (PubMed:9889278). Plays an essential role in innate immunity by promoting CGAS and RIGI activity (PubMed:30510222, PubMed:30804210). Participates in the DNA-triggered cGAS/STING pathway by promoting the DNA binding and activation of CGAS (PubMed:30510222). Triggers the condensation of cGAS, a process probably linked to the formation of membrane-less organelles (PubMed:34779554). Enhances also RIGI-induced type I interferon production probably by helping RIGI at sensing pathogenic RNA (PubMed:30804210). May also act as a phosphorylation-dependent sequence-specific endoribonuclease in vitro: Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR (PubMed:11604510).

Alternative names

Recommended products

Recombinant Human G3BP protein is a Human Full Length protein, in the 1 to 466 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Applications

SDS-PAGE, MS

Accession
Q13283-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Sequence info

Amino acid sequence

M V M E K P S P L L V G R E F V R Q Y Y T L L N Q A P D M L H R F Y G K N S S Y V H G G L D S N G K P A D A V Y G Q K E I H R K V M S Q N F T N C H T K I R H V D A H A T L N D G V V V Q V M G L L S N N N Q A L R R F M Q T F V L A P E G S V A N K F Y V H N D I F R Y Q D E V F G G F V T E P Q E E S E E E V E E P E E R Q Q T P E V V P D D S G T F Y D Q A V V S N D M E E H L E E P V A E P E P D P E P E P E Q E P V S E I Q E E K P E P V L E E T A P E D A Q K S S S P A P A D I A Q T V Q E D L R T F S W A S V T S K N L P P S G A V P V T G I P P H V V K V P A S Q P R P E S K P E S Q I P P Q R P Q R D Q R V R E Q R I N I P P Q R G P R P I R E A G E Q G D I E P R R M V R H P D S H Q L F I G N L P H E V D K S E L K D F F Q S Y G N V V E L R I N S G G K L P N F G F V V F D D S E P V Q K V L S N R P I M F R G E V R L N V E E K K T R A A R E G D R R D N R L R G P G G P R G G L G G G M R G P P R G G M V Q K P G F G V G R G L A P R Q V E H H H H H H

Accession

Q13283

Protein length

Full Length

Predicted molecular weight

53.2 kDa

Amino acids

1 to 466

Nature

Recombinant

Tags

His tag C-Terminus

Specifications

Form

Liquid

Additional notes

ab103304 was purified using conventional chromatography techniques.

General info

Function

Protein involved in various processes, such as stress granule formation and innate immunity (PubMed:12642610, PubMed:20180778, PubMed:23279204, PubMed:30510222, PubMed:30804210). Plays an essential role in stress granule formation (PubMed:12642610, PubMed:20180778, PubMed:23279204, PubMed:32302570, PubMed:32302571, PubMed:32302572, PubMed:34739333, PubMed:35977029, PubMed:36183834, PubMed:36279435, PubMed:36692217, PubMed:37379838). Stress granules are membraneless compartments that store mRNAs and proteins, such as stalled translation pre-initiation complexes, in response to stress (PubMed:12642610, PubMed:20180778, PubMed:23279204, PubMed:27022092, PubMed:32302570, PubMed:32302571, PubMed:32302572, PubMed:36279435, PubMed:37379838). Promotes formation of stress granules phase-separated membraneless compartment by undergoing liquid-liquid phase separation (LLPS) upon unfolded RNA-binding: functions as a molecular switch that triggers RNA-dependent LLPS in response to a rise in intracellular free RNA concentrations (PubMed:32302570, PubMed:32302571, PubMed:32302572, PubMed:34739333, PubMed:36279435, PubMed:36692217). Also acts as an ATP- and magnesium-dependent helicase: unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency (PubMed:9889278). Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA (PubMed:9889278). Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends (PubMed:9889278). Plays an essential role in innate immunity by promoting CGAS and RIGI activity (PubMed:30510222, PubMed:30804210). Participates in the DNA-triggered cGAS/STING pathway by promoting the DNA binding and activation of CGAS (PubMed:30510222). Triggers the condensation of cGAS, a process probably linked to the formation of membrane-less organelles (PubMed:34779554). Enhances also RIGI-induced type I interferon production probably by helping RIGI at sensing pathogenic RNA (PubMed:30804210). May also act as a phosphorylation-dependent sequence-specific endoribonuclease in vitro: Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR (PubMed:11604510).

Post-translational modifications

Phosphorylation of the acidic disordered region regulates stress granule assembly (PubMed:32302571, PubMed:32302572). RASA1-dependent phosphorylation of Ser-149 induces a conformational change that prevents self-association (PubMed:11604510, PubMed:12642610). Dephosphorylation after HRAS activation is required for stress granule assembly (PubMed:11604510, PubMed:12642610). Ser-149 phosphorylation induces partial nuclear localization (PubMed:11604510).

Subcellular localisation

Nucleus

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Product promise

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1 product image

  • SDS-PAGE - Recombinant Human G3BP protein (ab103304), expandable thumbnail

    SDS-PAGE - Recombinant Human G3BP protein (ab103304)

    SDS-PAGE analysis of Recombinant Human G3BP protein (ab103304), under reducing conditions. Proteins visualized by coomassie blue stain.

    Lane 1: Molecular Weight Standards

    Lane 2: 3 μg Recombinant Human G3BP protein

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Product protocols

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