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AB114893

Recombinant Human GAA protein (GST tag N-Terminus)

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Recombinant Human GAA protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 952 aa range, expressed in Wheat germ, with >80%, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

Lysosomal alpha-glucosidase, Acid maltase, Aglucosidase alfa, GAA

1 Images
SDS-PAGE - Recombinant Human GAA protein (GST tag N-Terminus) (AB114893)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human GAA protein (GST tag N-Terminus) (AB114893)

12.5% SDS-PAGE showing ab114893 at approximately 130.79kDa.
Stained with Coomassie Blue.

Key facts

Purity

>80%

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, SDS-PAGE, WB

applications

Biologically active

No

Accession

P10253

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB137068

Anti-GAA antibody [EPR4716(2)]

RabMAb|Recombinant|Trial Size

Western blot - Anti-GAA antibody [EPR4716(2)] (AB137068)

  • 0
  • 0
Assay Kits

AB252887

Lysosomal alpha-Glucosidase Activity Assay Kit (Fluorometric)

Enzyme activity assay - Lysosomal alpha-Glucosidase Activity Assay Kit (Fluorometric) (AB252887)

  • 0
  • 0
Proteins & Peptides

AB114285

Recombinant Human Heparan Sulfate Proteoglycan 2/Perlecan protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human Heparan Sulfate Proteoglycan 2/Perlecan protein (GST tag N-Terminus) (AB114285)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Recombinant protein</p>" } } }
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Sequence info

[{"sequence":"MGVRHPPCSHRLLAVCALVSLATAALLGHILLHDFLLVPRELSGSSPVLEETHPAHQQGASRPGPRDAQAHPGRPRAVPTQCDVPPNSRFDCAPDKAITQEQCEARGCCYIPAKQGLQGAQMGQPWCFFPPSYPSYKLENLSSSEMGYTATLTRTTPTFFPKDILTLRLDVMMETENRLHFTIKDPANRRYEVPLETPRVHSRAPSPLYSVEFSEEPFGVIVHRQLDGRVLLNTTVAPLFFADQFLQLSTSLPSQYITGLAEHLSPLMLSTSWTRITLWNRDLAPTPGANLYGSHPFYLALEDGGSAHGVFLLNSNAMDVVLQPSPALSWRSTGGILDVYIFLGPEPKSVVQQYLDVVGYPFMPPYWGLGFHLCRWGYSSTAITRQVVENMTRAHFPLDVQWNDLDYMDSRRDFTFNKDGFRDFPAMVQELHQGGRRYMMIVDPAISSSGPAGSYRPYDEGLRRGVFITNETGQPLIGKVWPGSTAFPDFTNPTALAWWEDMVAEFHDQVPFDGMWIDMNEPSNFIRGSEDGCPNNELENPPYVPGVVGGTLQAATICASSHQFLSTHYNLHNLYGLTEAIASHRALVKARGTRPFVISRSTFAGHGRYAGHWTGDVWSSWEQLASSVPEILQFNLLGVPLVGADVCGFLGNTSEELCVRWTQLGAFYPFMRNHNSLLSLPQEPYSFSEPAQQAMRKALTLRYALLPHLYTLFHQAHVAGETVARPLFLEFPKDSSTWTVDHQLLWGEALLITPVLQAGKAEVTGYFPLGTWYDLQTVPIEALGSLPPPPAAPREPAIHSEGQWVTLPAPLDTINVHLRAGYIIPLQGPGLTTTESRQQPMALAVALTKGGEARGELFWDDGESLEVLERGAYTQVIFLARNNTIVNELVRVTSEGAGLQLQKVTVLGVATAPQQVLSNGVPVSNFTYSPDTKVLDICVSLLMGEQFLVSWC","proteinLength":"Full Length","predictedMolecularWeight":"130.79 kDa","actualMolecularWeight":null,"aminoAcidEnd":952,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P10253","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

Additional notes

Glutathione Sepharose

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General info

Function

Essential for the degradation of glycogen in lysosomes (PubMed : 14695532, PubMed : 18429042, PubMed : 1856189, PubMed : 7717400). Has highest activity on alpha-1,4-linked glycosidic linkages, but can also hydrolyze alpha-1,6-linked glucans (PubMed : 29061980).

Sequence similarities

Belongs to the glycosyl hydrolase 31 family.

Post-translational modifications

The different forms of acid glucosidase are obtained by proteolytic processing.. Phosphorylation of mannose residues ensures efficient transport of the enzyme to the lysosomes via the mannose 6-phosphate receptor.

Subcellular localisation

Lysosome

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Product protocols

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Target data

Essential for the degradation of glycogen in lysosomes (PubMed : 14695532, PubMed : 18429042, PubMed : 1856189, PubMed : 7717400). Has highest activity on alpha-1,4-linked glycosidic linkages, but can also hydrolyze alpha-1,6-linked glucans (PubMed : 29061980).
See full target information Lysosomal alpha-glucosidase
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Product promise

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