Recombinant human Galectin 3 protein is a Human Full Length protein, in the 1 to 250 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- Yes
Amino acid sequence
A D N F S L H D A L S G S G N P N P Q G W P G A W G N Q P A G A G G Y P G A S Y P G A Y P G Q A P P G A Y P G Q A P P G A Y H G A P G A Y P G A P A P G V Y P G P P S G P G A Y P S S G Q P S A P G A Y P A T G P Y G A P A G P L I V P Y N L P L P G G V V P R M L I T I L G T V K P N A N R I A L D F Q R G N D V A F H F N P R F N E N N R R V I V C N T K L D N N W G R E E R Q S V F P F E S G K P F K I Q V L V E P D H F K V A V N D A H L L Q Y N H R V K K L N E I S K L G I S G D I D L T S A S Y T M I
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes.
Alternative names
MAC2, LGALS3, Galectin-3, Gal-3, 35 kDa lectin, Carbohydrate-binding protein 35, Galactose-specific lectin 3, Galactoside-binding protein, IgE-binding protein, L-31, Laminin-binding protein, Lectin L-29, Mac-2 antigen, CBP 35, GALBP
Recommended products
Recombinant human Galectin 3 protein is a Human Full Length protein, in the 1 to 250 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- Yes
- Biological activity
- The activity is determined by the ability to induce chemotaxis of human PBMCs at concentrations ranging from 2-220 μg/mL. Corresponding to a specific activity of 5 x 102 units/mg.
- Accession
- P17931-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute in water
- Concentration
- Storage buffer
Constituents: 0.29% Sodium chloride, 0.164% Sodium phosphate
Sequence info
Amino acid sequence
- A D N F S L H D A L S G S G N P N P Q G W P G A W G N Q P A G A G G Y P G A S Y P G A Y P G Q A P P G A Y P G Q A P P G A Y H G A P G A Y P G A P A P G V Y P G P P S G P G A Y P S S G Q P S A P G A Y P A T G P Y G A P A G P L I V P Y N L P L P G G V V P R M L I T I L G T V K P N A N R I A L D F Q R G N D V A F H F N P R F N E N N R R V I V C N T K L D N N W G R E E R Q S V F P F E S G K P F K I Q V L V E P D H F K V A V N D A H L L Q Y N H R V K K L N E I S K L G I S G D I D L T S A S Y T M I
- Accession
- P17931
- Protein length
- Full Length
- Predicted molecular weight
- 26 kDa
- Amino acids
- 1 to 250
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
Purity Approximately 97% as determined by:Analysis by RP-HPLC.Reducing and non-reducing SDS-PAGE.Endotoxin level as measured by LAL is <0.01ng/µg or <0.1EU/µg.
General info
- Function
Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Galectin-3 also known as LGALS3 is a protein belonging to the galectin family which are beta-galactoside-binding lectins. This protein has a molecular weight of about 30 kDa. It shows expression in various tissues including the heart liver and gastrointestinal tract. Galectin-3 plays a mechanical role in cell-cell adhesion cell-matrix interactions and modulating immune responses. Researchers often use galectin-3 ELISA kits to quantify its presence in different biological samples.
- Biological function summary
Galectin-3 participates in cell growth regulation apoptosis and immune system modulation. Galectin-3 does not typically integrate into larger molecular complexes but it interacts with a variety of cell surface and extracellular matrix molecules. Its function influences processes like cell differentiation and angiogenesis where it can act as a regulator leveraging its ability to bind glycans on cell surfaces and matrix components.
- Pathways
Galectin-3 contributes to the regulation of pathways such as the MAPK signaling pathway and the Wnt signaling pathway. In the MAPK pathway it influences cell proliferation and survival. Galectin-3 interacts with proteins like β-catenin in the Wnt pathway assisting in transcriptional regulation and cell fate determination. These pathways are vital for maintaining cellular homeostasis and responding to extracellular signals.
- Associated diseases and disorders
Galectin-3 has a strong connection to cancer and fibrosis. Its role in cancer involves promoting tumor cell survival metastasis and angiogenesis where it interacts with proteins like integrins to facilitate tumor progression. In fibrosis galectin-3 contributes to fibrotic tissue formation especially in conditions like idiopathic pulmonary fibrosis and cardiac fibrosis often associated with proteins such as TGF-β. Understanding these interactions helps in developing therapeutic strategies targeting galectin-3 in these diseases.
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1 product image
Western blot - Recombinant human Galectin 3 protein (ab78583)
ab78583 used in Western Blot. Figure: 1 ug under (+) reducing and (-) non-reducing conditions in a 4-20% Tris-Glycine gel stained with Coomassie Blue. Human Galectin-3 has a predicted MW of 26 kDa.
All lanes: Western blot - Recombinant human Galectin 3 protein (ab78583)
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