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AB78583

Recombinant human Galectin 3 protein

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Recombinant human Galectin 3 protein is a Human Full Length protein, in the 1 to 250 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS.

View Alternative Names

MAC2, LGALS3, Galectin-3, Gal-3, 35 kDa lectin, Carbohydrate-binding protein 35, Galactose-specific lectin 3, Galactoside-binding protein, IgE-binding protein, L-31, Laminin-binding protein, Lectin L-29, Mac-2 antigen, CBP 35, GALBP

1 Images
Western blot - Recombinant human Galectin 3 protein (AB78583)
  • WB

Unknown

Western blot - Recombinant human Galectin 3 protein (AB78583)

ab78583 used in Western Blot. Figure : 1 ug under (+) reducing and (-) non-reducing conditions in a 4-20% Tris-Glycine gel stained with Coomassie Blue. Human Galectin-3 has a predicted MW of 26 kDa.

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Western blot - Recombinant human Galectin 3 protein (ab78583)

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Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

The activity is determined by the ability to induce chemotaxis of human PBMCs at concentrations ranging from 2-220 μg/mL. Corresponding to a specific activity of 5 x 102 units/mg.

Accession

P17931

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute in water

Storage buffer

Constituents: 0.29% Sodium chloride, 0.164% Sodium phosphate

storage-buffer

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Primary Antibodies

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
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Sequence info

[{"sequence":"ADNFSLHDALSGSGNPNPQGWPGAWGNQPAGAGGYPGASYPGAYPGQAPPGAYPGQAPPGAYHGAPGAYPGAPAPGVYPGPPSGPGAYPSSGQPSAPGAYPATGPYGAPAGPLIVPYNLPLPGGVVPRMLITILGTVKPNANRIALDFQRGNDVAFHFNPRFNENNRRVIVCNTKLDNNWGREERQSVFPFESGKPFKIQVLVEPDHFKVAVNDAHLLQYNHRVKKLNEISKLGISGDIDLTSASYTMI","proteinLength":"Full Length","predictedMolecularWeight":"26 kDa","actualMolecularWeight":null,"aminoAcidEnd":250,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P17931","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Galectin-3 also known as LGALS3 is a protein belonging to the galectin family which are beta-galactoside-binding lectins. This protein has a molecular weight of about 30 kDa. It shows expression in various tissues including the heart liver and gastrointestinal tract. Galectin-3 plays a mechanical role in cell-cell adhesion cell-matrix interactions and modulating immune responses. Researchers often use galectin-3 ELISA kits to quantify its presence in different biological samples.
Biological function summary

Galectin-3 participates in cell growth regulation apoptosis and immune system modulation. Galectin-3 does not typically integrate into larger molecular complexes but it interacts with a variety of cell surface and extracellular matrix molecules. Its function influences processes like cell differentiation and angiogenesis where it can act as a regulator leveraging its ability to bind glycans on cell surfaces and matrix components.

Pathways

Galectin-3 contributes to the regulation of pathways such as the MAPK signaling pathway and the Wnt signaling pathway. In the MAPK pathway it influences cell proliferation and survival. Galectin-3 interacts with proteins like β-catenin in the Wnt pathway assisting in transcriptional regulation and cell fate determination. These pathways are vital for maintaining cellular homeostasis and responding to extracellular signals.

Galectin-3 has a strong connection to cancer and fibrosis. Its role in cancer involves promoting tumor cell survival metastasis and angiogenesis where it interacts with proteins like integrins to facilitate tumor progression. In fibrosis galectin-3 contributes to fibrotic tissue formation especially in conditions like idiopathic pulmonary fibrosis and cardiac fibrosis often associated with proteins such as TGF-β. Understanding these interactions helps in developing therapeutic strategies targeting galectin-3 in these diseases.
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Specifications

Form

Lyophilized

Additional notes

Purity Approximately 97% as determined by:Analysis by RP-HPLC.Reducing and non-reducing SDS-PAGE.Endotoxin level as measured by LAL is <0.01ng/μg or <0.1EU/μg.

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General info

Function

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus : acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus : acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes.
See full target information LGALS3
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Product promise

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