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AB89487

Recombinant human Galectin 3 protein (Active) (His tag N-Terminus)

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(5 Publications)

Recombinant human Galectin 3 protein (Active) (His tag N-Terminus) is a Human Full Length protein, in the 1 to 250 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS.

View Alternative Names

MAC2, LGALS3, Galectin-3, Gal-3, 35 kDa lectin, Carbohydrate-binding protein 35, Galactose-specific lectin 3, Galactoside-binding protein, IgE-binding protein, L-31, Laminin-binding protein, Lectin L-29, Mac-2 antigen, CBP 35, GALBP

1 Images
SDS-PAGE - Recombinant human Galectin 3 protein (Active) (His tag N-Terminus) (AB89487)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human Galectin 3 protein (Active) (His tag N-Terminus) (AB89487)

3ug of reduced ab89487 on SDS-PAGE, stained by Coomassie Blue.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

The ED50 for this effect is ≤ 15 ug/ml.

Measured by its ability to agglutinate human red blood cells.

Accession

P17931

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Complementary Products

Primary Antibodies

AB76245

Anti-Galectin 3 antibody [EP2775Y]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Galectin 3 antibody [EP2775Y] (AB76245)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Proteins & Peptides

AB283440

Recombinant Human Galectin 1 protein

SDS-PAGE - Recombinant Human Galectin 1 protein (AB283440)

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Proteins & Peptides

AB259406

Recombinant mouse IL-4 protein (Active)

Premium Bioactive

Functional Studies - Recombinant mouse IL-4 protein (Active) (AB259406)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MADNFSLHDA LSGSGNPNPQ GWPGAWGNQP AGAGGYPGAS YPGAYPGQAP PGAYPGQAPP GAYPGAPGAY PGAPAPGVYP GPPSGPGAYP SSGQPSATGA YPATGPYGAP AGPLIVPYNL PLPGGVVPRM LITILGTVKP NANRIALDFQ RGNDVAFHFN PRFNENNRRV IVCNTKLDNN WGREERQSVF PFESGKPFKI QVLVEPDHFK VAVNDAHLLQ YNHRVKKLNE ISKLGISGDI DLTSASYTMI","proteinLength":"Full Length","predictedMolecularWeight":"28.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":250,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P17931","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Galectin-3 also known as LGALS3 is a protein belonging to the galectin family which are beta-galactoside-binding lectins. This protein has a molecular weight of about 30 kDa. It shows expression in various tissues including the heart liver and gastrointestinal tract. Galectin-3 plays a mechanical role in cell-cell adhesion cell-matrix interactions and modulating immune responses. Researchers often use galectin-3 ELISA kits to quantify its presence in different biological samples.
Biological function summary

Galectin-3 participates in cell growth regulation apoptosis and immune system modulation. Galectin-3 does not typically integrate into larger molecular complexes but it interacts with a variety of cell surface and extracellular matrix molecules. Its function influences processes like cell differentiation and angiogenesis where it can act as a regulator leveraging its ability to bind glycans on cell surfaces and matrix components.

Pathways

Galectin-3 contributes to the regulation of pathways such as the MAPK signaling pathway and the Wnt signaling pathway. In the MAPK pathway it influences cell proliferation and survival. Galectin-3 interacts with proteins like β-catenin in the Wnt pathway assisting in transcriptional regulation and cell fate determination. These pathways are vital for maintaining cellular homeostasis and responding to extracellular signals.

Galectin-3 has a strong connection to cancer and fibrosis. Its role in cancer involves promoting tumor cell survival metastasis and angiogenesis where it interacts with proteins like integrins to facilitate tumor progression. In fibrosis galectin-3 contributes to fibrotic tissue formation especially in conditions like idiopathic pulmonary fibrosis and cardiac fibrosis often associated with proteins such as TGF-β. Understanding these interactions helps in developing therapeutic strategies targeting galectin-3 in these diseases.
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Specifications

Form

Liquid

Additional notes

Purified by using conventional chromatography techniques

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General info

Function

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus : acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus : acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes.
See full target information LGALS3
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Inflammation research : official journal of the European Histamine Research Society ... [et al.] 73:563-580 PubMed38411635

2024

Altered vacuole membrane protein 1 (VMP1) expression is associated with increased NLRP3 inflammasome activation and mitochondrial dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Stephanie R Zack,Meghana Venkatesan,Roman Nikolaienko,Ben Cook,Ronald Melki,Aleksey V Zima,Edward M Campbell

The Journal of biological chemistry 298:101743 PubMed35183508

2022

Nutrient-responsive O-GlcNAcylation dynamically modulates the secretion of glycan-binding protein galectin 3.

Applications

Unspecified application

Species

Unspecified reactive species

Mohit P Mathew,Lara K Abramowitz,Julie G Donaldson,John A Hanover

Autophagy 18:1020-1048 PubMed34612142

2021

LGALS3 (galectin 3) mediates an unconventional secretion of SNCA/α-synuclein in response to lysosomal membrane damage by the autophagic-lysosomal pathway in human midbrain dopamine neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin Burbidge,David J Rademacher,Jessica Mattick,Stephanie Zack,Andrea Grillini,Luc Bousset,Ochan Kwon,Konrad Kubicki,Alexander Simon,Ronald Melki,Edward M Campbell

Scientific reports 11:3718 PubMed33580170

2021

Targeting galectin-3 with a high-affinity antibody for inhibition of high-grade serous ovarian cancer and other MUC16/CA-125-expressing malignancies.

Applications

Unspecified application

Species

Unspecified reactive species

Marina Stasenko,Evan Smith,Oladapo Yeku,Kay J Park,Ian Laster,Kwangkook Lee,Sven Walderich,Elizabeth Spriggs,Bo Rueda,Britta Weigelt,Dmitriy Zamarin,Thapi Dharma Rao,David R Spriggs

The Journal of biological chemistry 289:10510-7 PubMed24567334

2014

Galectin-3 regulates desmoglein-2 and intestinal epithelial intercellular adhesion.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Jiang,Carl R Rankin,Porfirio Nava,Ronen Sumagin,Ryuta Kamekura,Sean R Stowell,Mingli Feng,Charles A Parkos,Asma Nusrat
View all publications
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