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AB98150

Recombinant Human GALM protein (His tag N-Terminus)

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Recombinant Human GALM protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 342 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

BLOCK25, GALM, Galactose mutarotase, Aldose 1-epimerase

1 Images
SDS-PAGE - Recombinant Human GALM protein (His tag N-Terminus) (AB98150)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human GALM protein (His tag N-Terminus) (AB98150)

15% SDS-PAGE analysis of 3μg ab98150

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q96C23

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMASVTRAVFGELPSGGGTVEKFQLQSDLLRVDIISWGCTITALEVKDRQGRASDVVLGFAELEGYLQKQPYFGAVIGRVANRIAKGTFKVDGKEYHLAINKEPNSLHGGVRGFDKVLWTPRVLSNGVQFSRISPDGEEGYPGELKVWVTYTLDGGELIVNYRAQASQATPVNLTNHSYFNLAGQASPNINDHEVTIEADTYLPVDETLIPTGEVAPVQGTAFDLRKPVELGKHLQDFHLNGFDHNFCLKGSKEKHFCARVHHAASGRVLEVYTTQPGVQFYTGNFLDGTLKGKNGAVYPKHSGFCLETQNWPDAVNQPRFPPVLLRPGEEYDHTTWFKFSVA","proteinLength":"Full Length","predictedMolecularWeight":"39.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":342,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q96C23","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GALM also known as galactose mutarotase is an enzyme that plays an important mechanical role in carbohydrate metabolism. This protein catalyzes the conversion between the alpha and beta anomers of galactose facilitating the proper utilization and assimilation of galactose in the body. GALM exhibits a molecular mass of approximately 37 kDa and shows expression in various tissues such as liver kidney and small intestine indicating its widespread involvement in galactose processing across different biological systems.
Biological function summary

This protein acts as a cornerstone in maintaining galactose equilibrium ensuring efficient Leloir pathway function. It does not constitute part of a complex but independently ensures rapid interconversion of galactose anomers effectively priming galactose for downstream metabolic processes. GALM's operation helps stabilize intracellular galactose concentrations reinforcing its role in metabolic balance.

Pathways

GALM holds significance in the Leloir pathway which is pivotal for galactose catabolism. This pathway utilizes the interconversion of galactose anomers to channel galactose into the glycolytic pathway for energy production. Through its actions GALM interrelates with other enzymes such as galactokinase and galactose-1-phosphate uridylyltransferase forming an interconnected network that maintains sugar metabolism efficiency.

GALM's dysfunction correlates with conditions like galactosemia and cataracts. Galactosemia arises from disruptions in galactose metabolism leading to toxic accumulation of galactose or its metabolites. Though more commonly linked with other proteins in the Leloir pathway such as galactose-1-phosphate uridylyltransferase defects in GALM also contribute to these metabolic imbalances. Researchers observe potential connections between GALM activity and dysregulated sugar processing in cataract formation highlighting the enzyme's clinical relevance.
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Specifications

Form

Liquid

Additional notes

ab98150 was purified by using conventional chromatography.

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General info

Function

Mutarotase that catalyzes the interconversion of beta-D-galactose and alpha-D-galactose during galactose metabolism (PubMed : 12753898). Beta-D-galactose is metabolized in the liver into glucose 1-phosphate, the primary metabolic fuel, by the action of four enzymes that constitute the Leloir pathway : GALM, GALK1 (galactokinase), GALT (galactose-1-phosphate uridylyltransferase) and GALE (UDP-galactose-4'-epimerase) (PubMed : 30451973). Involved in the maintenance of the equilibrium between the beta- and alpha-anomers of galactose, therefore ensuring a sufficient supply of the alpha-anomer for GALK1 (PubMed : 12753898). Also active on D-glucose although shows a preference for galactose over glucose (PubMed : 12753898).

Sequence similarities

Belongs to the aldose epimerase family.

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Product protocols

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Target data

Mutarotase that catalyzes the interconversion of beta-D-galactose and alpha-D-galactose during galactose metabolism (PubMed : 12753898). Beta-D-galactose is metabolized in the liver into glucose 1-phosphate, the primary metabolic fuel, by the action of four enzymes that constitute the Leloir pathway : GALM, GALK1 (galactokinase), GALT (galactose-1-phosphate uridylyltransferase) and GALE (UDP-galactose-4'-epimerase) (PubMed : 30451973). Involved in the maintenance of the equilibrium between the beta- and alpha-anomers of galactose, therefore ensuring a sufficient supply of the alpha-anomer for GALK1 (PubMed : 12753898). Also active on D-glucose although shows a preference for galactose over glucose (PubMed : 12753898).
See full target information GALM
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