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AB158492

Recombinant Human GALNS/Chondroitinase protein (GST tag N-Terminus)

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Recombinant Human GALNS/Chondroitinase protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 522 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

N-acetylgalactosamine-6-sulfatase, Chondroitinsulfatase, Galactose-6-sulfate sulfatase, N-acetylgalactosamine-6-sulfate sulfatase, Chondroitinase, GalN6S, GalNAc6S sulfatase, GALNS

1 Images
SDS-PAGE - Recombinant Human GALNS/Chondroitinase protein (GST tag N-Terminus) (AB158492)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human GALNS/Chondroitinase protein (GST tag N-Terminus) (AB158492)

ab158492 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB

applications

Biologically active

No

Accession

P34059

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB187516

Anti-GALNS/Chondroitinase antibody

Western blot - Anti-GALNS/Chondroitinase antibody (AB187516)

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Proteins & Peptides

AB132796

Recombinant Human AMCase protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human AMCase protein (GST tag N-Terminus) (AB132796)

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Proteins & Peptides

AB114563

Recombinant Human G-6-Pase protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human G-6-Pase protein (GST tag N-Terminus) (AB114563)

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Proteins & Peptides

AB131881

Recombinant Human OGT / O-Linked N-Acetylglucosamine Transferase protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human OGT / O-Linked N-Acetylglucosamine Transferase protein (GST tag N-Terminus) (AB131881)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as GALNS.
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Sequence info

[{"sequence":"MAAVVAATRWWQLLLVLSAAGMGASGAPQPPNILLLLMDDMGWGDLGVYGEPSRETPNLDRMAAEGLLFPNFYSANPLCSPSRAALLTGRLPIRNGFYTTNAHARNAYTPQEIVGGIPDSEQLLPELLKKAGYVSKIVGKWHLGHRPQFHPLKHGFDEWFGSPNCHFGPYDNKARPNIPVYRDWEMVGRYYEEFPINLKTGEANLTQIYLQEALDFIKRQARHHPFFLYWAVDATHAPVYASKPFLGTSQRGRYGDAVREIDDSIGKILELLQDLHVADNTFVFFTSDNGAALISAPEQGGSNGPFLCGKQTTFEGGMREPALAWWPGHVTAGQVSHQLGSIMDLFTTSLALAGLTPPSDRAIDGLNLLPTLLQGRLMDRPIFYYRGDTLMAATLGQHKAHFWTWTNSWENFRQGIDFCPGQNVSGVTTHNLEDHTKLPLIFHLGRDPGERFPLSFASAEYQEALSRITSVVQQHQEALVPAQPQLNVCNWAVMNWAPPGCEKLGKCLTPPESIPKKCLWSH","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":522,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P34059","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Chondroitinase also known as N-acetylgalactosamine-6-sulfatase (GALNS) is an enzyme involved in the degradation of glycosaminoglycans such as chondroitin sulfate and keratan sulfate. It is a lysosomal hydrolase enzyme that plays an important role in remodeling the extracellular matrix by cleaving sulfate groups from these molecules. The molecular mass of GALNS is approximately 60 kDa. GALNS expression primarily occurs in tissues like cartilage bones and connective tissues reflecting its function in skeletal development and maintenance.
Biological function summary

N-acetylgalactosamine-6-sulfatase acts as a critical player in catabolism breaking down complex polysaccharides in lysosomes. By degrading glycosaminoglycans it prevents accumulation of excessive polysaccharides in cells which might lead to cellular dysfunction. GALNS operates as a single molecule not forming any known complexes yet its activity is integral to maintaining cellular homeostasis and preventing pathological buildup of substrates within cells.

Pathways

GALNS functions in the glycosaminoglycan metabolism pathway essential for recycling cellular materials from the extracellular matrix. Another related pathway is the lysosomal degradation pathway where it relates to other enzymes like iduronate-2-sulfatase that also participate in the breakdown of complex molecules. These pathways collectively help in maintaining normal cellular function by facilitating the turnover of matrix components.

N-acetylgalactosamine-6-sulfatase relates to Morquio A syndrome a lysosomal storage disorder caused by deficiencies in GALNS activity. This leads to abnormal accumulation of keratan sulfate and chondroitin sulfate resulting in skeletal abnormalities and growth issues. GALNS has also connections to mucopolysaccharidosis IV where defects in this enzyme similarly cause buildup of glycosaminoglycans. Proteins like N-sulfoglucosamine sulfohydrolase show association with GALNS through shared pathways in these disorders highlighting the interconnected nature of enzyme functions in lysosomal storage diseases.
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Specifications

Form

Liquid

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General info

Sequence similarities

Belongs to the sulfatase family.

Post-translational modifications

The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity.

Subcellular localisation

Lysosome

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Product protocols

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Target data

See full target information N-acetylgalactosamine-6-sulfatase
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