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AB199572

Recombinant Human GALNT3 protein (His tag)

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Recombinant Human GALNT3 protein (His tag) is a Human Fragment protein, in the 38 to 633 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, HPLC.

View Alternative Names

Polypeptide N-acetylgalactosaminyltransferase 3, Polypeptide GalNAc transferase 3, Protein-UDP acetylgalactosaminyltransferase 3, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3, GalNAc-T3, pp-GaNTase 3, GALNT3

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

HPLC, SDS-PAGE

applications

Biologically active

No

Accession

Q14435

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: 99% Phosphate Buffer, 0.87% Sodium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"QREVSVQYSKEESRMERNMKNKNKMLDLMLEAVNNIKDAMPKMQIGAPVRQNIDAGERPCLQGYYTAAELKPVLDRPPQDSNAPGASGKAFKTTNLSVEEQKEKERGEAKHCFNAFASDRISLHRDLGPDTRPPECIEQKFKRCPPLPTTSVIIVFHNEAWSTLLRTVHSVLYSSPAILLKEIILVDDASVDEYLHDKLDEYVKQFSIVKIVRQRERKGLITARLLGATVATAETLTFLDAHCECFYGWLEPLLARIAENYTAVVSPDIASIDLNTFEFNKPSPYGSNHNRGNFDWSLSFGWESLPDHEKQRRKDETYPIKTPTFAGGLFSISKEYFEYIGSYDEEMEIWGGENIEMSFRVWQCGGQLEIMPCSVVGHVFRSKSPHSFPKGTQVIARNQVRLAEVWMDEYKEIFYRRNTDAAKIVKQKAFGDLSKRFEIKHRLQCKNFTWYLNNIYPEVYVPDLNPVISGYIKSVGQPLCLDVGENNQGGKPLIMYTCHGLGGNQYFEYSAQHEIRHNIQKELCLHAAQGLVQLKACTYKGHKTVVTGEQIWEIQKDQLLYNPFLKMCLSANGEHPSLVSCNPSDPLQKWILSQNDVDHHHHHH","proteinLength":"Fragment","predictedMolecularWeight":"69 kDa","actualMolecularWeight":null,"aminoAcidEnd":633,"aminoAcidStart":38,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q14435","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GALNT3 also known as polypeptide N-acetylgalactosaminyltransferase 3 is an enzyme that plays an important role in the O-glycosylation process. It transfers N-acetylgalactosamine (GalNAc) to serine and threonine residues of proteins. This enzyme belongs to the glycosyltransferase family and has a molecular mass of approximately 70 kDa. GALNT3 is mainly expressed in tissues such as liver kidney small intestine and bone.
Biological function summary

GALNT3 is responsible for initiating O-linked glycosylation of proteins affecting their stability function and localization. It acts alone and not as part of a larger protein complex. Through its activity GALNT3 modulates various proteins involved in cellular functions such as signal transduction and cell-cell interaction. This glycosylation process helps in the correct folding of proteins and protecting them from proteolytic degradation.

Pathways

The role of GALNT3 places it prominently in the glycosylation pathway where it influences the modification of proteins involved in the signaling cascade. One important pathway involving GALNT3 is the regulation of mineralization controlled by fibroblast growth factor 23 (FGF23). GALNT3 glycosylates and activates FGF23 highlighting its relationship to other proteins within the signaling axis including the fibroblast growth factor receptors (FGFRs).

Alterations in GALNT3 are associated with familial tumoral calcinosis a disorder characterized by abnormal calcium and phosphate metabolism. Mutations in GALNT3 disrupt its function leading to improper glycosylation and degradation of FGF23 resulting in elevated levels of phosphate. The connection between GALNT3 and FGF23 is central in understanding the pathogenesis of this condition as well as other metabolic bone diseases related to phosphate homeostasis.
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Specifications

Form

Liquid

Additional notes

ab199572 is greater than 95% pure as determined by SEC-HPLC and reducing SDS-PAGE.

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General info

Function

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor (PubMed : 16638743, PubMed : 31932717, PubMed : 8663203, PubMed : 9295285). Has activity toward HIV envelope glycoprotein gp120, EA2, MUC2, MUC1A and MUC5AC (PubMed : 8663203, PubMed : 9295285). Probably glycosylates fibronectin in vivo (PubMed : 9295285). Glycosylates FGF23 (PubMed : 16638743, PubMed : 31932717).

Sequence similarities

Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.

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Product protocols

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Target data

Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor (PubMed : 16638743, PubMed : 31932717, PubMed : 8663203, PubMed : 9295285). Has activity toward HIV envelope glycoprotein gp120, EA2, MUC2, MUC1A and MUC5AC (PubMed : 8663203, PubMed : 9295285). Probably glycosylates fibronectin in vivo (PubMed : 9295285). Glycosylates FGF23 (PubMed : 16638743, PubMed : 31932717).
See full target information Polypeptide N-acetylgalactosaminyltransferase 3
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