Recombinant Human gamma Actin protein (GST tag N-Terminus)

• WB

Lab

Western blot - Recombinant Human gamma Actin protein (GST tag N-Terminus) (AB157841)

ab213262 was shown to react with ACTNB (beta actin) when tested against HAP1 wild-type and HAP1 ACTNB (beta actin) knockout cells. ACTNB wild-type and knockout samples were subjected to SDS-PAGE and incubated with ab213262. Incomplete reduction of signal was seen in the ACTNB (beta actin) knockout samples at beta actin's expected molecular weight. SDS-PAGE of gamma actin recombinant protein and incubation with ab213262 showed cross-reactivity to gamma actin. ab213262 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at a 1ug/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-beta Actin antibody [EPR21241] - Loading Control (<a href='/en-us/products/primary-antibodies/beta-actin-antibody-epr21241-loading-control-ab213262'>ab213262</a>) at 1 µg/mL

Lane 1:

HAP1 whole cell lysate at 20 µg

Lane 2:

HAP1 ACTNB knockout whole cell lysate at 20 µg

Lane 3:

MEF1 whole cell lysate at 20 µg

Lane 4:

NIH3T3 whole cell lysate at 20 µg

Lane 5:

Western blot - Recombinant Human gamma Actin protein (GST tag N-Terminus) (ab157841) at 0.1 µg

Predicted band size: 41 kDa

false

• WB

Lab

Western blot - Recombinant Human gamma Actin protein (GST tag N-Terminus) (AB157841)

ab241153 was shown to react with ACTNB (beta actin) when tested against HAP1 wild-type and HAP1 ACTNB (beta actin) knockout cells. ACTNB wild-type and knockout samples were subjected to SDS-PAGE and incubated with ab231153. Incomplete reduction of signal was seen in the ACTNB (beta actin) knockout samples at beta actin's expected molecular weight. SDS-PAGE of gamma actin recombinant protein and incubation with ab241153 showed cross-reactivity to gamma actin. ab241153 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at a 1ug/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-beta Actin antibody [EPR21242] - Loading Control (<a href='/en-us/products/primary-antibodies/beta-actin-antibody-epr21242-loading-control-ab241153'>ab241153</a>)

Lane 1:

HAP1 whole cell lysate at 20 µg

Lane 2:

HAP1 ACTNB whole cell lysate at 20 µg

Lane 3:

NIH3T3 whole cell lysate at 20 µg

Lane 4:

MEF whole cell lysate at 20 µg

Lane 5:

Western blot - Recombinant Human gamma Actin protein (GST tag N-Terminus) (ab157841)

Predicted band size: 41 kDa

false

• ELISA

Lab

ELISA - Recombinant Human gamma Actin protein (GST tag N-Terminus) (AB157841)

1 ug per mL of recombinant protein ab157838 and ab157841 were immobilised in PBS on an ELISA plate overnight. After blocking in 5% BSA, primary antibody (ab119952) was added in a concentration range of 0.017-1000 ng per mL.

Pre-absorbed secondary antibody goat anti-rabbit IgG H&L (HRP,ab97080) was used at 1/20000 dilution.

• SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human gamma Actin protein (GST tag N-Terminus) (AB157841)

ab157841 on a 12.5% SDS-PAGE stained with Coomassie Blue.