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Recombinant Human gamma Actin protein is a Human Full Length protein, in the 1 to 375 aa range, expressed in Wheat germ and suitable for WB, ELISA.

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Images

ELISA - Recombinant Human gamma Actin protein (AB157841), expandable thumbnail
  • Western blot - Recombinant Human gamma Actin protein (AB157841), expandable thumbnail
  • Western blot - Recombinant Human gamma Actin protein (AB157841), expandable thumbnail
  • SDS-PAGE - Recombinant Human gamma Actin protein (AB157841), expandable thumbnail

Key facts

Expression system
Wheat germ
Tags
GST tag N-Terminus
Applications
WB, ELISA
Biologically active
No

Amino acid sequence

M E E E I A A L V I D N G S G M C K A G F A G D D A P R A V F P S I V G R P R H Q G V M V G M G Q K D S Y V G D E A Q S K R G I L T L K Y P I E H G I V T N W D D M E K I W H H T F Y N E L R V A P E E H P V L L T E A P L N P K A N R E K M T Q I M F E T F N T P A M Y V A I Q A V L S L Y A S G R T T G I V M D S G D G V T H T V P I Y E G Y A L P H A I L R L D L A G R D L T D Y L M K I L T E R G Y S F T T T A E R E I V R D I K E K L C Y V A L D F E Q E M A T A A S S S S L E K S Y E L P D G Q V I T I G N E R F R C P E A L F Q P S F L G M E S C G I H E T T F N S I M K C D V D I R K D L Y A N T V L S G G T T M Y P G I A D R M Q K E I T A L A P S T M K I K I I A P P E R K Y S V W I G G S I L A S L S T F Q Q M W I S K Q E Y D E S G P S I V H R K C F

Reactivity data

Application
WB
Reactivity
Reacts
Dilution info
-
Notes

-

Application
ELISA
Reactivity
Reacts
Dilution info
1 µg/mL
Notes

-

Target data

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. May play a role in the repair of noise-induced stereocilia gaps thereby maintains hearing sensitivity following loud noise damage (By similarity).

Alternative names

ACTG, ACTG1, Gamma-actin

Recommended products

Recombinant Human gamma Actin protein is a Human Full Length protein, in the 1 to 375 aa range, expressed in Wheat germ and suitable for WB, ELISA.

Key facts

Expression system
Wheat germ
Applications
WB, ELISA
Accession
P63261-1
Animal free
No
Species
Human
Concentration
Loading...
Storage buffer

pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione

Sequence info

Amino acid sequence

M E E E I A A L V I D N G S G M C K A G F A G D D A P R A V F P S I V G R P R H Q G V M V G M G Q K D S Y V G D E A Q S K R G I L T L K Y P I E H G I V T N W D D M E K I W H H T F Y N E L R V A P E E H P V L L T E A P L N P K A N R E K M T Q I M F E T F N T P A M Y V A I Q A V L S L Y A S G R T T G I V M D S G D G V T H T V P I Y E G Y A L P H A I L R L D L A G R D L T D Y L M K I L T E R G Y S F T T T A E R E I V R D I K E K L C Y V A L D F E Q E M A T A A S S S S L E K S Y E L P D G Q V I T I G N E R F R C P E A L F Q P S F L G M E S C G I H E T T F N S I M K C D V D I R K D L Y A N T V L S G G T T M Y P G I A D R M Q K E I T A L A P S T M K I K I I A P P E R K Y S V W I G G S I L A S L S T F Q Q M W I S K Q E Y D E S G P S I V H R K C F
Accession
P63261
Protein length
Full Length
Amino acids
1 to 375
Nature
Recombinant
Tags
GST tag N-Terminus

Specifications

Form
Liquid

General info

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. May play a role in the repair of noise-induced stereocilia gaps thereby maintains hearing sensitivity following loud noise damage (By similarity).

Sequence similarities

Belongs to the actin family.

Post-translational modifications

Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization.

Subcellular localisation
Cytoskeleton

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

U+00A0;

U+00A0;

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Gamma actin also known as actin gamma 1 and actin kDa is an essential actin protein found in the cytoskeleton. It is expressed widely in various tissues including muscle and non-muscle cells. This protein has a molecular mass of approximately 42 kDa. Gamma actin plays a mechanical role in maintaining cellular shape and facilitating cell motility by polymerizing into filamentous structures known as F-actin. It has alternate names such as 2a-3' and actin green in some contexts.

Biological function summary

Gamma actin contributes significantly to the structure of the cytoskeleton affecting cell shape and function. It forms part of actin filament complexes that provide tension and support to the cell. In both muscle and non-muscle cells gamma actin interacts with other actin isoforms to ensure filament stability and elasticity. These interactions are important for processes like cell division signal transduction and cellular transport.

Pathways

Gamma actin is involved in pathways related to cell motility and division. This protein is integral to the actin cytoskeleton pathway and has connections with proteins such as profilin and cofilin which regulate actin filament dynamics. Additionally it plays roles in signal transduction pathways that control cellular responses to external signals working alongside proteins like the Rho GTPases.

Associated diseases and disorders

Gamma actin has been linked to conditions such as deafness and certain types of cancer. Mutations in the ACTG1 gene encoding gamma actin have been associated with hereditary hearing loss. In cancer changes in gamma actin expression can affect cell migration and invasion contributing to tumor progression. The protein's interaction with other actins and related proteins like actin-binding proteins makes it a critical factor in the molecular mechanisms underlying these diseases.

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