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Recombinant Human GAPDH protein is a Human Full Length protein, in the 1 to 335 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
Alternative names=Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, OK/SW-cl.12, CDABP0047, GAPD, GAPDH
- Purity
>95% SDS-PAGE
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE
- Biologically active
No
- Sequence
M G K V K V G V N G F G R I G R L V T R A A F N S G K V D I V A I N D P F I D L N Y M V Y M F Q Y D S T H G K F H G T V K A E N G K L V I N G N P I T I F Q E R D P S K I K W G D A G A E Y V V E S T G V F T T M E K A G A H L Q G G A K R V I I S A P S A D A P M F V M G V N H E K Y D N S L K I I S N A S C T T N C L A P L A K V I H D N F G I V E G L M T T V H A I T A T Q K T V D G P S G K L W R D G R G A L Q N I I P A S T G A A K A V G K V I P E L N G K L T G M A F R V P T A N V S V V D L T C R L E K P A K Y D D I K K V V K Q A S E G P L K G I L G Y T E H Q V V S S D F N S D T H S S T F D A G A G I A L N D H F V K L I S W Y D N E F G Y S N R V V D L M A H M A S K E
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Images
Target data
Function
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively (PubMed:3170585, PubMed:11724794). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (PubMed:3170585, PubMed:11724794). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed:23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed:23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed:23332158, PubMed:27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity).
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Recombinant Human GAPDH protein is a Human Full Length protein, in the 1 to 335 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
Alternative names=Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, OK/SW-cl.12, CDABP0047, GAPD, GAPDH
Key facts
- Purity
>95% SDS-PAGE
- Expression system
Escherichia coli
- Tags
Tag free
- Applications
SDS-PAGE
- Biologically active
No
- Predicted molecular weight
36 kDa
- Amino acids
1 to 335
- Sequence
M G K V K V G V N G F G R I G R L V T R A A F N S G K V D I V A I N D P F I D L N Y M V Y M F Q Y D S T H G K F H G T V K A E N G K L V I N G N P I T I F Q E R D P S K I K W G D A G A E Y V V E S T G V F T T M E K A G A H L Q G G A K R V I I S A P S A D A P M F V M G V N H E K Y D N S L K I I S N A S C T T N C L A P L A K V I H D N F G I V E G L M T T V H A I T A T Q K T V D G P S G K L W R D G R G A L Q N I I P A S T G A A K A V G K V I P E L N G K L T G M A F R V P T A N V S V V D L T C R L E K P A K Y D D I K K V V K Q A S E G P L K G I L G Y T E H Q V V S S D F N S D T H S S T F D A G A G I A L N D H F V K L I S W Y D N E F G Y S N R V V D L M A H M A S K E
- Accession
- P04406-1
- Protein length
Full Length
- Animal free
No
- Nature
Recombinant
- Species
Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.242% Tris, 0.0292% EDTA, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Specifications
- Form
Liquid
- Additional notes
ab77109 is purified by using conventional chromatography techniques.
General info
- Function
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively (PubMed:3170585, PubMed:11724794). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (PubMed:3170585, PubMed:11724794). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed:23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed:23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed:23332158, PubMed:27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity).
- Sequence similarities
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
- Post-translational modifications
S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus (By similarity). S-nitrosylation of Cys-247 is induced by interferon-gamma and LDL(ox) implicating the iNOS-S100A8/9 transnitrosylase complex and seems to prevent interaction with phosphorylated RPL13A and to interfere with GAIT complex activity (PubMed:22771119, PubMed:25417112).
- Subcellular localisation
Nucleus, Cytoskeleton
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
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