Recombinant Human GAPDS protein is a Human Full Length protein, in the 1 to 408 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M S K R D I V L T N V T V V Q L L R Q P C P V T R A P P P P E P K A E V E P Q P Q P E P T P V R E E I K P P P P P L P P H P A T P P P K M V S V A R E L T V G I N G F G R I G R L V L R A C M E K G V K V V A V N D P F I D P E Y M V Y M F K Y D S T H G R Y K G S V E F R N G Q L V V D N H E I S V Y Q C K E P K Q I P W R A V G S P Y V V E S T G V Y L S I Q A A S D H I S A G A Q R V V I S A P S P D A P M F V M G V N E N D Y N P G S M N I V S N A S C T T N C L A P L A K V I H E R F G I V E G L M T T V H S Y T A T Q K T V D G P S R K A W R D G R G A H Q N I I P A S T G A A K A V T K V I P E L K G K L T G M A F R V P T P D V S V V D L T C R L A Q P A P Y S A I K E A V K A A A K G P M A G I L A Y T E D E V V S T D F L G D T H S S I F D A K A G I A L N D N F V K L I S W Y D N E Y G Y S H R V V D L L R Y M F S R D K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
May play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. Required for sperm motility and male fertility (By similarity).
Alternative names
GAPD2, GAPDH2, GAPDS, HSD-35, HSD35, GAPDHS, Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2, Spermatogenic glyceraldehyde-3-phosphate dehydrogenase, GAPDH-2
Recommended products
Recombinant Human GAPDS protein is a Human Full Length protein, in the 1 to 408 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- O14556-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M S K R D I V L T N V T V V Q L L R Q P C P V T R A P P P P E P K A E V E P Q P Q P E P T P V R E E I K P P P P P L P P H P A T P P P K M V S V A R E L T V G I N G F G R I G R L V L R A C M E K G V K V V A V N D P F I D P E Y M V Y M F K Y D S T H G R Y K G S V E F R N G Q L V V D N H E I S V Y Q C K E P K Q I P W R A V G S P Y V V E S T G V Y L S I Q A A S D H I S A G A Q R V V I S A P S P D A P M F V M G V N E N D Y N P G S M N I V S N A S C T T N C L A P L A K V I H E R F G I V E G L M T T V H S Y T A T Q K T V D G P S R K A W R D G R G A H Q N I I P A S T G A A K A V T K V I P E L K G K L T G M A F R V P T P D V S V V D L T C R L A Q P A P Y S A I K E A V K A A A K G P M A G I L A Y T E D E V V S T D F L G D T H S S I F D A K A G I A L N D N F V K L I S W Y D N E Y G Y S H R V V D L L R Y M F S R D K
- Accession
- O14556
- Protein length
- Full Length
- Amino acids
- 1 to 408
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
May play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. Required for sperm motility and male fertility (By similarity).
- Sequence similarities
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as GAPDHS.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GAPDS also known as glyceraldehyde-3-phosphate dehydrogenase-sperm is an important enzyme involved in glycolytic processes. The protein usually references a mass of about 44 kDa. Expressed mainly in testicular cells and spermatozoa GAPDS is important for energy production during spermatogenesis and sperm motility. It shares sequence similarity with GAPDH making it an isoform within the same enzymatic family.
- Biological function summary
GAPDS plays a critical role in glycolysis serving as an important enzyme that facilitates the conversion of glyceraldehyde-3-phosphate to 13-bisphosphoglycerate. GAPDS can function in complex with other glycolytic enzymes helping generate ATP which is necessary for sperm cell functions. GAPDS expression supports processes essential for male fertility specifically through its involvement in energy supply to sperm flagella.
- Pathways
GAPDS is a part of the glycolysis metabolic pathway which is essential for ATP production in sperm. In this pathway it works closely with other glycolytic enzymes like phosphoglycerate kinase and enolase ensuring the continuous flow of energy. GAPDS also indirectly connects to the respiratory chain as glycolytic byproducts feed into mitochondrial oxidative phosphorylation allowing integrated energy metabolism.
- Associated diseases and disorders
GAPDS has implications in male infertility particularly when there is abnormal sperm motility. Defects or deficiencies in GAPDS can impair ATP production in sperm leading to suboptimal motility and reduced fertility. The protein might also have a connection with certain metabolic disorders that affect energy production like glycolytic enzyme deficiencies where similar pathways involving proteins like GAPDH might show concurrent dysfunctions.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human GAPDS protein (ab161972)
ab161972 on a 12.5% SDS-PAGE stained with Coomassie Blue.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com