Recombinant Human Gastrin protein (His-tag) is a Human Full Length protein, expressed in Escherichia coli, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for MS, SDS-PAGE.
Images
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- MS, SDS-PAGE
- Biologically active
- No
- Mass spectrometry
- LC-MS/MS
Amino acid sequence
S W K P R S Q Q P D A P L G T G A N R D L E L P W L E Q Q G P A S H H R R Q L G P Q G P P H L V A D P S K K Q G P W L E E E E E A Y G W M D F G R R S A E D E N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine.
Alternative names
GAS, GAST, Gastrin
Recommended products
Recombinant Human Gastrin protein (His-tag) is a Human Full Length protein, expressed in Escherichia coli, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for MS, SDS-PAGE.
Key facts
- Purity
- >=95% HPLC
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- MS, SDS-PAGE
- Biologically active
- No
- Mass spectrometry
- LC-MS/MS
- Accession
- P01350-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 10% Trehalose, 0.3355% Sodium phosphate dibasic heptahydrate, 0.0698% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- S W K P R S Q Q P D A P L G T G A N R D L E L P W L E Q Q G P A S H H R R Q L G P Q G P P H L V A D P S K K Q G P W L E E E E E A Y G W M D F G R R S A E D E N
- Accession
- P01350
- Protein length
- Full Length
- Predicted molecular weight
- 28.28 kDa
- Actual molecular weight
- 28.15 kDa
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Lyophilized
General info
- Function
Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine.
- Sequence similarities
Belongs to the gastrin/cholecystokinin family.
- Post-translational modifications
Two different processing pathways probably exist in antral G-cells. In the dominant pathway progastrin is cleaved at three sites resulting in two major bioactive gastrins, gastrin-34 and gastrin-17. In the putative alternative pathway, progastrin may be processed only at the most C-terminal dibasic site resulting in the synthesis of gastrin-71.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
Notes
Please note that this protein has a HIS IF2D1 tag at N-terminal.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Gastrin sometimes referred to as gastrin peptide is a hormone consisting of 34 amino acids with a molecular mass of approximately 3.8 kDa. This protein is mainly expressed in the G cells of the stomach lining specifically the antrum but also found in the duodenum and pancreas. Gastrin operates by stimulating the secretion of gastric acid from parietal cells in the stomach playing an important role in the digestive process. The presence of gastrin can be detected through various methods like gastrin stain and antibodies such as anti-gastrin are often used for this purpose.
- Biological function summary
Gastrin impacts digestion and is an important promoter of gastric acid secretion and mucosal growth. Gastrin does not assemble into larger protein complexes functioning instead as an independent peptide hormone. It exerts its effects by binding to the cholecystokinin B receptor (CCK-B) on target cells leading to increased intracellular calcium levels and activation of protein kinases. The interaction initiates a cascade of events facilitating digestion by optimizing the stomach's environment.
- Pathways
Gastrin has a significant role in the gastrointestinal (GI) pathway where it activates the secretion of hydrochloric acid. It also influences the phosphoinositide pathway through its relationship with G-proteins. The hormonal action of gastrin involves several other proteins like somatostatin which serves as an inhibitory counterpart and histamine that works synergistically by promoting further acid secretion through different receptors.
- Associated diseases and disorders
Physicians often study gastrin in relation to conditions like Zollinger-Ellison syndrome and peptic ulcers. Zollinger-Ellison syndrome leads to excessive production of gastrin causing severe ulcerations and gastric acid secretions. Concurrently elevated gastrin levels sometimes associate with tumors like gastrinomas. The interplay between gastrin and H. pylori infection is pivotal for peptic ulcer development where bacteria-induced inflammation could alter gastrin's normal regulatory functions of acid secretion.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human Gastrin protein (His-tag) (ab322314)
SDS-PAGE analysis of ab322314 under reducing conditions for 2ug protein.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com