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Recombinant Human GATA3 protein is a Human Full Length protein, in the 2 to 443 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, FuncS.

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Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, FuncS

Biologically active

No

Amino acid sequence

M A S M T G G Q Q M G R G H H H H H H E N L Y F Q G E V T A D Q P R W V S H H H P A V L N G Q H P D T H H P G L S H S Y M D A A Q Y P L P E E V D V L F N I D G Q G N H V P P Y Y G N S V R A T V Q R Y P P T H H G S Q V C R P P L L H G S L P W L D G G K A L G S H H T A S P W N L S P F S K T S I H H G S P G P L S V Y P P A S S S S L S G G H A S P H L F T F P P T P P K D V S P D P S L S T P G S A G S A R Q D E K E C L K Y Q V P L P D S M K L E S S H S R G S M T A L G G A S S S T H H P I T T Y P P Y V P E Y S S G L F P P S S L L G G S P T G F G C K S R P K A R S S T G R E C V N C G A T S T P L W R R D G T G H Y L C N A C G L Y H K M N G Q N R P L I K P K R R L S A A R R A G T S C A N C Q T T T T T L W R R N A N G D P V C N A C G L Y Y K L H N I N R P L T M K K E G I Q T R N R K M S S K S K K C K K V H D S L E D F P K N S S F N P A A L S R H M S S L S H I S P F S H S S H M L T T P T P M H P P S S L S F G P H H P S S M V T A M G

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

FuncS

Reactivity

Reacts

Dilution info

-

Notes

1. May be used for in vitro GATA3 mediated gene transcription regulation for T cell's differentiation.

2. May be used as specific protein substrate for kinase and ubiquitin (Sumo pathway) related enzyme functional screening assays.

3. May be used for GATA3 protein-protein interaction mapping.

4. As immunogen for specific antibody production.

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses. Positively regulates ASB2 expression (By similarity). Coordinates macrophage transcriptional activation and UCP2-dependent metabolic reprogramming in response to IL33. Upon tissue injury, acts downstream of IL33 signaling to drive differentiation of inflammation-resolving alternatively activated macrophages.

Alternative names

Recommended products

Recombinant Human GATA3 protein is a Human Full Length protein, in the 2 to 443 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, FuncS.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Applications

SDS-PAGE, FuncS

Accession
P23771-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 8
Constituents: EDTA, Glycerol (glycerin, glycerine), L-Arginine, Potassium chloride, Sodium chloride, 0.32% Tris HCl

Sequence info

Amino acid sequence

M A S M T G G Q Q M G R G H H H H H H E N L Y F Q G E V T A D Q P R W V S H H H P A V L N G Q H P D T H H P G L S H S Y M D A A Q Y P L P E E V D V L F N I D G Q G N H V P P Y Y G N S V R A T V Q R Y P P T H H G S Q V C R P P L L H G S L P W L D G G K A L G S H H T A S P W N L S P F S K T S I H H G S P G P L S V Y P P A S S S S L S G G H A S P H L F T F P P T P P K D V S P D P S L S T P G S A G S A R Q D E K E C L K Y Q V P L P D S M K L E S S H S R G S M T A L G G A S S S T H H P I T T Y P P Y V P E Y S S G L F P P S S L L G G S P T G F G C K S R P K A R S S T G R E C V N C G A T S T P L W R R D G T G H Y L C N A C G L Y H K M N G Q N R P L I K P K R R L S A A R R A G T S C A N C Q T T T T T L W R R N A N G D P V C N A C G L Y Y K L H N I N R P L T M K K E G I Q T R N R K M S S K S K K C K K V H D S L E D F P K N S S F N P A A L S R H M S S L S H I S P F S H S S H M L T T P T P M H P P S S L S F G P H H P S S M V T A M G

Accession

P23771

Protein length

Full Length

Predicted molecular weight

50.76 kDa

Amino acids

2 to 443

Nature

Recombinant

Specifications

Form

Liquid

Additional notes

refolded and chromatographically purified.

General info

Function

Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. Required for the T-helper 2 (Th2) differentiation process following immune and inflammatory responses. Positively regulates ASB2 expression (By similarity). Coordinates macrophage transcriptional activation and UCP2-dependent metabolic reprogramming in response to IL33. Upon tissue injury, acts downstream of IL33 signaling to drive differentiation of inflammation-resolving alternatively activated macrophages.

Subcellular localisation

Nucleus

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-80°C

Appropriate long-term storage conditions

-80°C

Storage information

Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

GATA3 also known as GATA-binding factor 3 is a transcription factor with a molecular mass of about 50 kDa. It belongs to the GATA family of zinc finger transcription factors. GATA3 is mainly expressed in the immune system especially in T-cells as well as in the nervous system and mammary glands. It plays a role in the regulation of critical developmental processes and cell lineage commitment through its binding to the 'GATA' motif in the promoter regions of target genes.

Biological function summary

GATA3 functions as a regulator of gene expression involved in various differentiation processes. It is not a solitary molecule; it interacts with other proteins to form transcriptional complexes. In T-helper type 2 (Th2) cells GATA3 is necessary for Th2 cell differentiation and cytokine production. Its expression is vital for the development of the nervous system and mammary gland morphogenesis highlighting its role in diverse biological contexts.

Pathways

GATA3 plays a significant role in the Th2 differentiation pathway and is linked to the immune response. It acts in association with other transcription factors like STAT6 and T-bet. GATA3 promotes the transcription of Th2 cytokines such as IL-4 IL-5 and IL-13 while suppressing Th1 responses. This balance orchestrated by GATA3 is important for immune homeostasis and the body's ability to respond to infections and allergens.

Associated diseases and disorders

The dysregulation of GATA3 is associated with atopic diseases and breast cancer. Aberrant expression of GATA3 is often observed in allergic conditions due to its role in Th2 cell differentiation and cytokine expression. In the context of breast cancer GATA3 levels can impact tumor progression and are correlated with estrogen receptor status. Additionally its relation with other breast cancer-related proteins like L50 underlines its importance in cancer biology and potential as a therapeutic target.

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