JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB112289

Recombinant Human GATA4 protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human GATA4 protein is a Human Fragment protein, in the 343 to 442 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

Transcription factor GATA-4, GATA-binding factor 4, GATA4

1 Images
SDS-PAGE - Recombinant Human GATA4 protein (AB112289)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human GATA4 protein (AB112289)

ab112289 analysed on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

Tag free

Applications

WB, ELISA, SDS-PAGE

applications

Biologically active

No

Accession

P43694

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

useful for Antibody Production and Protein Array
style
left-column

Sequence info

[{"sequence":"ASGASSNSSNATTSSSEEMRPIKTEPGLSSHYGHSSSVSQTFSVSAMSGHGPSIHPVLSALKLSPQGYASPVSQSPQTSSKQDSWNSLVLADSHGDIITA","proteinLength":"Fragment","predictedMolecularWeight":"36.63 kDa","actualMolecularWeight":null,"aminoAcidEnd":442,"aminoAcidStart":343,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P43694","tags":[]}]
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GATA4 also known as GATA-binding protein 4 is a transcription factor that plays a significant role in gene expression regulation. It weighs approximately 44 kDa. This protein expresses mainly in cells of the heart gonads and liver. GATA4 functions through binding to the GATA sequence on DNA regulating various genes. The protein is well known for its involvement in cardiac development and function.
Biological function summary

GATA4 interacts with other transcription factors and co-factors to regulate developmental processes. It often forms complexes with proteins such as NKX2-5 and TBX5 influencing the expression of cardiac-specific genes. These interactions lead to tissue-specific gene expression control important in the differentiation and functioning of heart cells. GATA4 is also involved in the regulation of genes responsible for cell growth and apoptosis contributing to tissue homeostasis.

Pathways

GATA4 is a notable component of cardiac development and cellular differentiation pathways. It plays an integral role in the Wnt signaling pathway influencing heart development through gene transcription modification. GATA4 also collaborates with proteins such as MEF2 and BMP2 coordinating cardiac muscle differentiation. These pathways highlight its pivotal role in proper cardiovascular formation and function.

GATA4 mutations and dysregulation have links to congenital heart defects such as atrial septal defect (ASD). This condition associates with altered gene expression during heart development leading to structural abnormalities. GATA4 also relates to conditions like hypertrophic cardiomyopathy where its interaction with proteins like MYH7 suggests a complex network influencing cardiac hypertrophy. Understanding these connections is essential for developing targeted therapeutic strategies.
style
left-column

Specifications

Form

Liquid

style
left-column

General info

Function

Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development and function (PubMed : 24000169, PubMed : 27984724, PubMed : 35182466). In cooperation with TBX5, it binds to cardiac super-enhancers and promotes cardiomyocyte gene expression, while it down-regulates endocardial and endothelial gene expression (PubMed : 27984724). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression. Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (PubMed : 20081228). Required during testicular development (PubMed : 21220346). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, sphingosine-1-phosphate lyase (PubMed : 15734735).

Post-translational modifications

Methylation at Lys-300 attenuates transcriptional activity.

Subcellular localisation

Nucleus

style
left-column

Product protocols

style
left-column

Target data

Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development and function (PubMed : 24000169, PubMed : 27984724, PubMed : 35182466). In cooperation with TBX5, it binds to cardiac super-enhancers and promotes cardiomyocyte gene expression, while it down-regulates endocardial and endothelial gene expression (PubMed : 27984724). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression. Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (PubMed : 20081228). Required during testicular development (PubMed : 21220346). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, sphingosine-1-phosphate lyase (PubMed : 15734735).
See full target information GATA4
style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB302761

Human GATA4 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human GATA4 ELISA Kit (AB302761)

  • 0
  • 0
Primary Antibodies

AB307823

Anti-GATA4 antibody [EPR26718-103]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-GATA4 antibody [EPR26718-103] (AB307823)

  • 0
  • 0
Proteins & Peptides

AB204202

Recombinant Human GATA3 protein

  • 0
  • 0
Proteins & Peptides

AB95933

Recombinant human XIAP protein

Functional Studies - Recombinant human XIAP protein (AB95933)

  • 0
  • 0
Proteins & Peptides

AB112374

Recombinant Human Tristetraprolin/TTP protein

SDS-PAGE - Recombinant Human Tristetraprolin/TTP protein (AB112374)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com