Recombinant Human GBA3 protein (His tag) is a Human Full Length protein, in the 1 to 469 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
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Key facts
- Purity
>85% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M A F P A G F G W A A A T A A Y Q V E G G W D A D G K G P C V W D T F T H Q G G E R V F K N Q T G D V A C G S Y T L W E E D L K C I K Q L G L T H Y R F S L S W S R L L P D G T T G F I N Q K G I D Y Y N K I I D D L L K N G V T P I V T L Y H F D L P Q T L E D Q G G W L S E A I I E S F D K Y A Q F C F S T F G D R V K Q W I T I N E A N V L S V M S Y D L G M F P P G I P H F G T G G Y Q A A H N L I K A H A R S W H S Y D S L F R K K Q K G M V S L S L F A V W L E P A D P N S V S D Q E A A K R A I T F H L D L F A K P I F I D G D Y P E V V K S Q I A S M S Q K Q G Y P S S R L P E F T E E E K K M I K G T A D F F A V Q Y Y T T R L I K Y Q E N K K G E L G I L Q D A E I E F F P D P S W K N V D W I Y V V P W G V C K L L K Y I K D T Y N N P V I Y I T E N G F P Q S D P A P L D D T Q R W E Y F R Q T F Q E L F K A I Q L D K V N L Q V Y C A W S L L D N F E W N Q G Y S S R F G L F H V D F E D P A R P R V P Y T S A K E Y A K I I R N N G L E A H L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Neutral cytosolic beta-glycosidase with a broad substrate specificity that could play a role in the catabolism of glycosylceramides (PubMed:11389701, PubMed:11784319, PubMed:17595169, PubMed:20728381, PubMed:26724485, PubMed:33361282). Has a significant glucosylceramidase activity in vitro (PubMed:17595169, PubMed:26724485). However, that activity is relatively low and its significance in vivo is not clear (PubMed:17595169, PubMed:20728381, PubMed:26724485). Hydrolyzes galactosylceramides/GalCers, glucosylsphingosines/GlcSphs and galactosylsphingosines/GalSphs (PubMed:17595169). However, the in vivo relevance of these activities is unclear (PubMed:17595169). It can also hydrolyze a broad variety of dietary glycosides including phytoestrogens, flavonols, flavones, flavanones and cyanogens in vitro and could therefore play a role in the metabolism of xenobiotics (PubMed:11784319). Possesses transxylosylase activity in vitro using xylosylated ceramides/XylCers (such as beta-D-xylosyl-(1<->1')-N-acylsphing-4-enine) as xylosyl donors and cholesterol as acceptor (PubMed:33361282). Could also play a role in the catabolism of cytosolic sialyl free N-glycans (PubMed:26193330).
Alternative names
CBG, CBGL1, GBA3, Cytosolic beta-glucosidase, Cytosolic beta-glucosidase-like protein 1, Cytosolic galactosylceramidase, Cytosolic glucosylceramidase, Cytosolic glycosylceramidase, Glucosidase beta acid 3, Glucosylceramidase beta 3, Klotho-related protein, Cytosolic GCase, KLrP
Recommended products
Recombinant Human GBA3 protein (His tag) is a Human Full Length protein, in the 1 to 469 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
>85% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- Q9H227-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine), 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M A F P A G F G W A A A T A A Y Q V E G G W D A D G K G P C V W D T F T H Q G G E R V F K N Q T G D V A C G S Y T L W E E D L K C I K Q L G L T H Y R F S L S W S R L L P D G T T G F I N Q K G I D Y Y N K I I D D L L K N G V T P I V T L Y H F D L P Q T L E D Q G G W L S E A I I E S F D K Y A Q F C F S T F G D R V K Q W I T I N E A N V L S V M S Y D L G M F P P G I P H F G T G G Y Q A A H N L I K A H A R S W H S Y D S L F R K K Q K G M V S L S L F A V W L E P A D P N S V S D Q E A A K R A I T F H L D L F A K P I F I D G D Y P E V V K S Q I A S M S Q K Q G Y P S S R L P E F T E E E K K M I K G T A D F F A V Q Y Y T T R L I K Y Q E N K K G E L G I L Q D A E I E F F P D P S W K N V D W I Y V V P W G V C K L L K Y I K D T Y N N P V I Y I T E N G F P Q S D P A P L D D T Q R W E Y F R Q T F Q E L F K A I Q L D K V N L Q V Y C A W S L L D N F E W N Q G Y S S R F G L F H V D F E D P A R P R V P Y T S A K E Y A K I I R N N G L E A H L
- Accession
- Protein length
Full Length
- Predicted molecular weight
56.1 kDa
- Amino acids
1 to 469
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
- Additional notes
ab187481 was purified using conventional chromatography techniques.
General info
- Function
Neutral cytosolic beta-glycosidase with a broad substrate specificity that could play a role in the catabolism of glycosylceramides (PubMed:11389701, PubMed:11784319, PubMed:17595169, PubMed:20728381, PubMed:26724485, PubMed:33361282). Has a significant glucosylceramidase activity in vitro (PubMed:17595169, PubMed:26724485). However, that activity is relatively low and its significance in vivo is not clear (PubMed:17595169, PubMed:20728381, PubMed:26724485). Hydrolyzes galactosylceramides/GalCers, glucosylsphingosines/GlcSphs and galactosylsphingosines/GalSphs (PubMed:17595169). However, the in vivo relevance of these activities is unclear (PubMed:17595169). It can also hydrolyze a broad variety of dietary glycosides including phytoestrogens, flavonols, flavones, flavanones and cyanogens in vitro and could therefore play a role in the metabolism of xenobiotics (PubMed:11784319). Possesses transxylosylase activity in vitro using xylosylated ceramides/XylCers (such as beta-D-xylosyl-(1<->1')-N-acylsphing-4-enine) as xylosyl donors and cholesterol as acceptor (PubMed:33361282). Could also play a role in the catabolism of cytosolic sialyl free N-glycans (PubMed:26193330).
- Sequence similarities
Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.
- Post-translational modifications
The N-terminus is blocked.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GBA3 also known as cytosolic beta-glucosidase is an enzyme with a molecular mass of approximately 60 kDa. This protein functions to hydrolyze glycosides specifically beta-D-glucosides and other saccharide derivatives facilitating the breakdown of these molecules into useful substrates for the cell. GBA3 is expressed in various tissues such as liver kidney and small intestine indicating its widespread role in metabolism.
- Biological function summary
Enzymes like cytosolic beta-glucosidase contribute to essential digestive and metabolic processes by breaking down glycosidic bonds found in complex carbohydrates. They are not part of a larger protein complex but they cooperate with other enzymes to maintain energy production from dietary sources. This activity helps the organism to manage carbohydrate metabolism effectively releasing simple sugars that cells use for energy.
- Pathways
GBA3 interacts closely with glycolysis and gluconeogenesis important pathways for energy homeostasis. In these pathways GBA3's role in carbohydrate breakdown supplies simple sugars that enter further stages of energy production. It acts alongside proteins like glucokinase and phosphoglucomutase which also contribute to these metabolic pathways aiding in the management of energy resources in the body.
- Associated diseases and disorders
Abnormal activity or expression of GBA3 can link to metabolic conditions like Gaucher's disease and Niemann-Pick disease. These are lysosomal storage disorders where disruptions in glycoside processing can lead to harmful accumulation of glucosylceramides. Proteins such as glucocerebrosidase (GBA1) are also affected in these disorders highlighting a network where GBA3 activity is vital for preventing substrate buildup and maintaining cellular health.
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SDS-PAGE - Recombinant Human GBA3 protein (His tag) (ab187481)
15% SDS-PAGE analysis of 3μg ab187481.
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