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AB217830

Recombinant Human GC-F protein

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Recombinant Human GC-F protein is a Human Fragment protein, in the 367 to 1108 aa range, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for SDS-PAGE, WB.

View Alternative Names

GUC2F, RETGC2, GUCY2F, Retinal guanylyl cyclase 2, RETGC-2, Guanylate cyclase F, Rod outer segment membrane guanylate cyclase 2, GC-F, ROS-GC2

1 Images
SDS-PAGE - Recombinant Human GC-F protein (AB217830)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human GC-F protein (AB217830)

SDS-PAGE analysis of ab217830.

Key facts

Purity

>70% Densitometry

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

WB, SDS-PAGE

applications

Biologically active

No

Accession

P51841

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.04% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.03% EDTA, 0.02% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Previously labelled as PGCG.
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Sequence info

[{"sequence":"MKENGQAGAASLVQHSRNMQFHGFNQLMRTDSNGNGISEYVILDTNLKEWELHSTYTVDMEMELLRFGGTPIHFPGGRPPRADAKCWFAEGKICHGGIDPAFAMMVCLTLLIALLSINGFAYFIRRRINKIQLIKGPNRILLTLEDVTFINPHFGSKRGSRASVSFQITSEVQSGRSPRLSFSSGSLTPATYENSNIAIYEGDWVWLKKFSLGDFGDLKSIKSRASDVFEMMKDLRHENINPLLGFFYDSGMFAIVTEFCSRGSLEDILTNQDVKLDWMFKSSLLLDLIKGMKYLHHREFVHGRLKSRNCVVDGRFVLKVTDYGFNDILEMLRLSEEESSMEELLWTAPELLRAPRGSRLGSFAGDVYSFAIIMQEVMVRGTPFCMMDLPAQEIINRLKKPPPVYRPVVPPEHAPPECLQLMKQCWAEAAEQRPTFDEIFNQFKTFNKGKKTNIIDSMLRMLEQYSSNLEDLIRERTEELEIEKQKTEKLLTQMLPPSVAESLKKGCTVEPEGFDLVTLYFSDIVGFTTISAMSEPIEVVDLLNDLYTLFDAIIGSHDVYKVETIGDAYMVASGLPKRNGSRHAAEIANMSLDILSSVGTFKMRHMPEVPVRIRIGLHSGPVVAGVVGLTMPRYCLFGDTVNTASRMESTGLPYRIHVSLSTVTILQNLSEGYEVELRGRTELKGKGTEETFWLIGKKGFMKPLPVPPPVDKDGQVGHGLQPVEIAAFQRRKAERQLVRNKP","proteinLength":"Fragment","predictedMolecularWeight":"110 kDa","actualMolecularWeight":null,"aminoAcidEnd":1108,"aminoAcidStart":367,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"P51841","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target GC-F protein also known as Guanylate Cyclase F is a critical component in cellular signaling. GC-F protein has a molecular mass around 120 kDa. Found mainly in the retina GC-F expresses in photoreceptor cells where it plays a role in light detection. The protein is a part of a larger family of guanylate cyclases which are responsible for converting GTP to cGMP an essential secondary messenger in many signal transduction pathways.
Biological function summary

GC-F influences processes in vision by regulating cGMP levels which affects ion channels and cellular activity in response to light. GC-F operates largely in the retina and does not function in isolation; it is part of a complex that involves other retinal-specific proteins. This complex includes components necessary for the stability and location of GC-F within photoreceptor cells.

Pathways

GC-F protein is deeply integrated into the phototransduction pathway. This pathway translates light stimuli into electrical signals. The protein interacts both directly and indirectly with key players such as rhodopsin and the cGMP-gated ion channels. Another pathway involves GC-F's participation in regulating neuroprotective responses linking it to proteins like PKG which mediate cellular responses to cGMP levels.

GC-F is connected mainly to retinal diseases. Dysregulation or mutation of the GC-F can lead to retinal dystrophies such as retinitis pigmentosa which results in progressive vision loss. Other inherited retinal diseases also showcase GC-F's disruption often involving proteins like rhodopsin and peripherin/RDS that further complicate the pathology. Understanding GC-F's role provides insights into mechanisms of these disorders paving the way for therapeutic interventions.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Responsible for the synthesis of cyclic GMP (cGMP) in rods and cones of photoreceptors (PubMed : 7777544). Plays an essential role in phototransduction, by mediating cGMP replenishment (By similarity). May also participate in the trafficking of membrane-asociated proteins to the photoreceptor outer segment membrane (By similarity).

Sequence similarities

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.

Post-translational modifications

There are 9 conserved cysteine residues in sensory guanylate cyclases, 6 in the extracellular domain, which may be involved in intra- or interchain disulfide bonds.

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Product protocols

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Target data

Responsible for the synthesis of cyclic GMP (cGMP) in rods and cones of photoreceptors (PubMed : 7777544). Plays an essential role in phototransduction, by mediating cGMP replenishment (By similarity). May also participate in the trafficking of membrane-asociated proteins to the photoreceptor outer segment membrane (By similarity).
See full target information GUCY2F
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