Recombinant Human GCAT protein (denatured) is a Human Full Length protein, in the 22 to 419 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M S A L A Q L R G I L E G E L E G I R G A G T W K S E R V I T S R Q G P H I R V D G V S G G I L N F C A N N Y L G L S S H P E V I Q A G L Q A L E E F G A G L S S V R F I C G T Q S I H K N L E A K I A R F H Q R E D A I L Y P S C Y D A N A G L F E A L L T P E D A V L S D E L N H A S I I D G I R L C K A H K Y R Y R H L D M A D L E A K L Q E A Q K H R L R L V A T D G A F S M D G D I A P L Q E I C C L A S R Y G A L V F M D E C H A T G F L G P T G R G T D E L L G V M D Q V T I I N S T L G K A L G G A S G G Y T T G P G P L V S L L R Q R A R P Y L F S N S L P P A V V G C A S K A L D L L M G S N T I V Q S M A A K T Q R F R S K M E A A G F T I S G A S H P I C P V M L G D A R L A S R M A D D M L K R G I F V I G F S Y P V V P K G K A R I R V Q I S A V H S E E D I D R C V E A F V E V G R L H G A L P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Pyridoxal phosphate (PLP) dependent enzyme, which catalyzes the cleavage of 2-amino-3-oxobutanoate to glycine and acetyl-CoA.
Alternative names
KBL, GCAT, AKB ligase, Aminoacetone synthase, Glycine acetyltransferase
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Recombinant Human GCAT protein (denatured) is a Human Full Length protein, in the 22 to 419 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- O75600-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M S A L A Q L R G I L E G E L E G I R G A G T W K S E R V I T S R Q G P H I R V D G V S G G I L N F C A N N Y L G L S S H P E V I Q A G L Q A L E E F G A G L S S V R F I C G T Q S I H K N L E A K I A R F H Q R E D A I L Y P S C Y D A N A G L F E A L L T P E D A V L S D E L N H A S I I D G I R L C K A H K Y R Y R H L D M A D L E A K L Q E A Q K H R L R L V A T D G A F S M D G D I A P L Q E I C C L A S R Y G A L V F M D E C H A T G F L G P T G R G T D E L L G V M D Q V T I I N S T L G K A L G G A S G G Y T T G P G P L V S L L R Q R A R P Y L F S N S L P P A V V G C A S K A L D L L M G S N T I V Q S M A A K T Q R F R S K M E A A G F T I S G A S H P I C P V M L G D A R L A S R M A D D M L K R G I F V I G F S Y P V V P K G K A R I R V Q I S A V H S E E D I D R C V E A F V E V G R L H G A L P
- Accession
- O75600
- Protein length
- Full Length
- Predicted molecular weight
- 45 kDa
- Amino acids
- 22 to 419
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Pyridoxal phosphate (PLP) dependent enzyme, which catalyzes the cleavage of 2-amino-3-oxobutanoate to glycine and acetyl-CoA.
- Sequence similarities
Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.
- Subcellular localisation
- Mitochondrion, Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GCAT also known as glycine C-acetyltransferase functions as a mitochondrial enzyme involved in glycine metabolism. It has a molecular mass of approximately 44 kDa. Researchers often find GCAT expressed in the liver where it plays a significant role in metabolic processes related to amino acid synthesis and catabolism. Being predominantly cytosolic GCAT contributes to the detoxification pathways by participating in the conversion of glycine to other metabolites.
- Biological function summary
In glycine cleavage system GCAT operates as part of a complex essential for the regulation of amino acid levels. The enzyme accepts substrates from reactions facilitated by other components such as T-protein and P-protein and is vital in maintaining the balance of glycine and other amino acids within the cell. Dysfunction in any part of this system can result in elevated levels of toxic metabolic intermediates leading to cellular stress and potential tissue damage.
- Pathways
GCAT is an integral element in the mitochondrial glycine cleavage system linking it to broader metabolic pathways such as the Krebs cycle and oxidative phosphorylation. This position between pathways underlines its connection with proteins such as glycine dehydrogenase and aminomethyltransferase. This interconnectedness suggests that imbalances involving GCAT can disrupt metabolic homeostasis influencing energy production at the cellular level.
- Associated diseases and disorders
Defects or dysregulation in GCAT activity link to various metabolic conditions including nonketotic hyperglycinemia and liver diseases. Patients with nonketotic hyperglycinemia show accumulation of glycine leading to neurological issues conditions typically associated with deficiencies in glycine metabolism enzymes like P-protein. In the context of liver diseases alterations in GCAT's function affect the liver's ability to process nitrogen highlighting its importance in liver homeostasis and function.
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1 product image
SDS-PAGE - Recombinant Human GCAT protein (denatured) (ab156971)
15% SDS-PAGE analysis of ab156971 (3 µg).
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