Recombinant Human GCDH/GCD protein is a Human Full Length protein, in the 45 to 438 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M R P E F D W Q D P L V L E E Q L T T D E I L I R D T F R T Y C Q E R L M P R I L L A N R N E V F H R E I I S E M G E L G V L G P T I K G Y G C A G V S S V A Y G L L A R E L E R V D S G Y R S A M S V Q S S L V M H P I Y A Y G S E E Q R Q K Y L P Q L A K G E L L G C F G L T E P N S G S D P S S M E T R A H Y N S S N K S Y T L N G T K T W I T N S P M A D L F V V W A R C E D G C I R G F L L E K G M R G L S A P R I Q G K F S L R A S A T G M I I M D G V E V P E E N V L P G A S S L G G P F G C L N N A R Y G I A W G V L G A S E F C L H T A R Q Y A L D R M Q F G V P L A R N Q L I Q K K L A D M L T E I T L G L H A C L Q L G R L K D Q D K A A P E M V S L L K R N N C G K A L D I A R Q A R D M L G G N G I S D E Y H V I R H A M N L E A V N T Y E G T H D I H A L I L G R A I T G I Q A F T A S K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. Isoform Short is inactive.
Alternative names
GCD, GCDH
Recommended products
Recombinant Human GCDH/GCD protein is a Human Full Length protein, in the 45 to 438 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q92947-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 1.16% Sodium chloride, 0.316% Tris HCl, 0.077% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M R P E F D W Q D P L V L E E Q L T T D E I L I R D T F R T Y C Q E R L M P R I L L A N R N E V F H R E I I S E M G E L G V L G P T I K G Y G C A G V S S V A Y G L L A R E L E R V D S G Y R S A M S V Q S S L V M H P I Y A Y G S E E Q R Q K Y L P Q L A K G E L L G C F G L T E P N S G S D P S S M E T R A H Y N S S N K S Y T L N G T K T W I T N S P M A D L F V V W A R C E D G C I R G F L L E K G M R G L S A P R I Q G K F S L R A S A T G M I I M D G V E V P E E N V L P G A S S L G G P F G C L N N A R Y G I A W G V L G A S E F C L H T A R Q Y A L D R M Q F G V P L A R N Q L I Q K K L A D M L T E I T L G L H A C L Q L G R L K D Q D K A A P E M V S L L K R N N C G K A L D I A R Q A R D M L G G N G I S D E Y H V I R H A M N L E A V N T Y E G T H D I H A L I L G R A I T G I Q A F T A S K
- Accession
- Q92947
- Protein length
- Full Length
- Predicted molecular weight
- 45.8 kDa
- Amino acids
- 45 to 438
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab98118 is purified by using conventional chromatography techniques.
General info
- Function
Catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. Isoform Short is inactive.
- Sequence similarities
Belongs to the acyl-CoA dehydrogenase family.
- Subcellular localisation
- Mitochondrion matrix
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as GCDH
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GCDH also known as glutaryl-CoA dehydrogenase is an enzyme involved in the catabolic breakdown of specific amino acids. This protein harbors a mass of approximately 45 kDa and functions primarily within the mitochondria. GCDH expression occurs in various tissues including the liver kidney and brain. The enzyme plays a mechanical role by catalyzing the decarboxylation of glutaryl-CoA to crotonyl-CoA facilitating the removal of carbon dioxide in this biochemical process.
- Biological function summary
This enzyme acts as an important component in the lysine hydroxylysine and tryptophan degradation pathways. GCDH does not form part of a larger enzyme complex but performs its function independently ensuring the proper degradation and metabolism of amino acids. Its activity aids in maintaining metabolic homeostasis preventing the accumulation of harmful intermediates within cells. GCDH may interact with cofactors like flavin adenine dinucleotide (FAD) to carry out its enzymatic activity.
- Pathways
GCDH plays a significant role in the lysine and tryptophan metabolism pathways. Within these pathways GCDH helps convert glutaryl-CoA a molecule derived from amino acid catabolism into intermediates ultimately leading to energy production. The enzyme's functionality connects with other mitochondrial enzymes like isovaleryl-CoA dehydrogenase (IVD) which also participates in amino acid breakdown. Proper interaction and coordination within these pathways are essential for maintaining metabolic balance in the cell.
- Associated diseases and disorders
GCDH is closely linked to glutaric aciduria type 1 (GA1) an inborn error of metabolism. This disorder arises from mutations in the GCDH gene leading to insufficient enzyme activity and subsequent buildup of glutaric acid. This accumulation can cause severe neurological damage over time if untreated. GA1 can show genetic connections with other metabolic disorders involving enzymes like medium-chain acyl-CoA dehydrogenase (MCAD). Understanding these interactions helps in diagnosing and managing such metabolic disorders.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human GCDH/GCD protein (ab98118)
SDS-PAGE analysis of ab98118 (3µg) under reducing condition and visualized by coomassie blue stain.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com