Recombinant Human GCKR protein (Tagged) is a Human Full Length protein, in the 1 to 625 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
Amino acid sequence
M P G T K R F Q H V I E T P E P G K W E L S G Y E A A V P I T E K S N P L T Q D L D K A D A E N I V R L L G Q C D A E I F Q E E G Q A L S T Y Q R L Y S E S I L T T M V Q V A G K V Q E V L K E P D G G L V V L S G G G T S G R M A F L M S V S F N Q L M K G L G Q K P L Y T Y L I A G G D R S V V A S R E G T E D S A L H G I E E L K K V A A G K K R V I V I G I S V G L S A P F V A G Q M D C C M N N T A V F L P V L V G F N P V S M A R N D P I E D W S S T F R Q V A E R M Q K M Q E K Q K A F V L N P A I G P E G L S G S S R M K G G S A T K I L L E T L L L A A H K T V D Q G I A A S Q R C L L E I L R T F E R A H Q V T Y S Q S P K I A T L M K S V S T S L E K K G H V Y L V G W Q T L G I I A I M D G V E C I H T F G A D F R D V R G F L I G D H S D M F N Q K A E L T N Q G P Q F T F S Q E D F L T S I L P S L T E I D T V V F I F T L D D N L T E V Q T I V E Q V K E K T N H I Q A L A H S T V G Q T L P I P L K K L F P S I I S I T W P L L F F E Y E G N F I Q K F Q R E L S T K W V L N T V S T G A H V L L G K I L Q N H M L D L R I S N S K L F W R A L A M L Q R F S G Q S K A R C I E S L L R A I H F P Q P L S D D I R A A P I S C H V Q V A H E K E Q V I P I A L L S L L F R C S I T E A Q A H L A A A P S V C E A V R S A L A G P G Q K R T A D P L E I L E P D V Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Regulates glucokinase (GCK) by forming an inactive complex with this enzyme (PubMed:23621087, PubMed:23733961). Acts by promoting GCK recruitment to the nucleus, possibly to provide a reserve of GCK that can be quickly released in the cytoplasm after a meal (PubMed:10456334). The affinity of GCKR for GCK is modulated by fructose metabolites: GCKR with bound fructose 6-phosphate has increased affinity for GCK, while GCKR with bound fructose 1-phosphate has strongly decreased affinity for GCK and does not inhibit GCK activity (PubMed:23621087, PubMed:23733961).
Alternative names
Glucokinase regulatory protein, GKRP, Glucokinase regulator, GCKR
Recommended products
Recombinant Human GCKR protein (Tagged) is a Human Full Length protein, in the 1 to 625 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
>90% SDS-PAGE
- Expression system
Escherichia coli
- Tags
His tag N-Terminus
- Applications
SDS-PAGE
- Biologically active
No
- Accession
- Q14397-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
M P G T K R F Q H V I E T P E P G K W E L S G Y E A A V P I T E K S N P L T Q D L D K A D A E N I V R L L G Q C D A E I F Q E E G Q A L S T Y Q R L Y S E S I L T T M V Q V A G K V Q E V L K E P D G G L V V L S G G G T S G R M A F L M S V S F N Q L M K G L G Q K P L Y T Y L I A G G D R S V V A S R E G T E D S A L H G I E E L K K V A A G K K R V I V I G I S V G L S A P F V A G Q M D C C M N N T A V F L P V L V G F N P V S M A R N D P I E D W S S T F R Q V A E R M Q K M Q E K Q K A F V L N P A I G P E G L S G S S R M K G G S A T K I L L E T L L L A A H K T V D Q G I A A S Q R C L L E I L R T F E R A H Q V T Y S Q S P K I A T L M K S V S T S L E K K G H V Y L V G W Q T L G I I A I M D G V E C I H T F G A D F R D V R G F L I G D H S D M F N Q K A E L T N Q G P Q F T F S Q E D F L T S I L P S L T E I D T V V F I F T L D D N L T E V Q T I V E Q V K E K T N H I Q A L A H S T V G Q T L P I P L K K L F P S I I S I T W P L L F F E Y E G N F I Q K F Q R E L S T K W V L N T V S T G A H V L L G K I L Q N H M L D L R I S N S K L F W R A L A M L Q R F S G Q S K A R C I E S L L R A I H F P Q P L S D D I R A A P I S C H V Q V A H E K E Q V I P I A L L S L L F R C S I T E A Q A H L A A A P S V C E A V R S A L A G P G Q K R T A D P L E I L E P D V Q
- Accession
- Protein length
Full Length
- Predicted molecular weight
84.7 kDa
- Amino acids
1 to 625
- Nature
Recombinant
- Tags
His tag N-Terminus
Specifications
- Form
Liquid
General info
- Function
Regulates glucokinase (GCK) by forming an inactive complex with this enzyme (PubMed:23621087, PubMed:23733961). Acts by promoting GCK recruitment to the nucleus, possibly to provide a reserve of GCK that can be quickly released in the cytoplasm after a meal (PubMed:10456334). The affinity of GCKR for GCK is modulated by fructose metabolites: GCKR with bound fructose 6-phosphate has increased affinity for GCK, while GCKR with bound fructose 1-phosphate has strongly decreased affinity for GCK and does not inhibit GCK activity (PubMed:23621087, PubMed:23733961).
- Sequence similarities
Belongs to the GCKR family.
- Subcellular localisation
Nucleus, Mitochondrion
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
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1 product image
SDS-PAGE - Recombinant Human GCKR protein (Tagged) (ab225652)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab225652 with 5% enrichment gel and 15% separation gel.
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