Recombinant human GCN5L2 protein (Active)

Specifications

Form

Liquid

Additional notes

Affinity purified.

General info

Function

Protein lysine acyltransferase that can act as a acetyltransferase, glutaryltransferase, succinyltransferase or malonyltransferase, depending on the context (PubMed : 29211711, PubMed : 35995428). Acts as a histone lysine succinyltransferase : catalyzes succinylation of histone H3 on 'Lys-79' (H3K79succ), with a maximum frequency around the transcription start sites of genes (PubMed : 29211711). Succinylation of histones gives a specific tag for epigenetic transcription activation (PubMed : 29211711). Association with the 2-oxoglutarate dehydrogenase complex, which provides succinyl-CoA, is required for histone succinylation (PubMed : 29211711). In different complexes, functions either as an acetyltransferase (HAT) or as a succinyltransferase : in the SAGA and ATAC complexes, acts as a histone acetyltransferase (PubMed : 17301242, PubMed : 19103755, PubMed : 29211711). Has significant histone acetyltransferase activity with core histones, but not with nucleosome core particles (PubMed : 17301242, PubMed : 19103755, PubMed : 21131905). Has a a strong preference for acetylation of H3 at 'Lys-9' (H3K9ac) (PubMed : 21131905). Acetylation of histones gives a specific tag for epigenetic transcription activation (PubMed : 17301242, PubMed : 19103755, PubMed : 29211711). Recruited by the XPC complex at promoters, where it specifically mediates acetylation of histone variant H2A.Z.1/H2A.Z, thereby promoting expression of target genes (PubMed : 29973595, PubMed : 31527837). Involved in long-term memory consolidation and synaptic plasticity : acts by promoting expression of a hippocampal gene expression network linked to neuroactive receptor signaling (By similarity). Acts as a positive regulator of T-cell activation : upon TCR stimulation, recruited to the IL2 promoter following interaction with NFATC2 and catalyzes acetylation of histone H3 at 'Lys-9' (H3K9ac), leading to promote IL2 expression (By similarity). Required for growth and differentiation of craniofacial cartilage and bone by regulating acetylation of histone H3 at 'Lys-9' (H3K9ac) (By similarity). Regulates embryonic stem cell (ESC) pluripotency and differentiation (By similarity). Also acetylates non-histone proteins, such as CEBPB, MRE11, PPARGC1A, PLK4 and TBX5 (PubMed : 16753578, PubMed : 17301242, PubMed : 27796307, PubMed : 29174768, PubMed : 38128537). Involved in heart and limb development by mediating acetylation of TBX5, acetylation regulating nucleocytoplasmic shuttling of TBX5 (PubMed : 29174768). Acts as a negative regulator of centrosome amplification by mediating acetylation of PLK4 (PubMed : 27796307). Acts as a negative regulator of gluconeogenesis by mediating acetylation and subsequent inactivation of PPARGC1A (PubMed : 16753578, PubMed : 23142079). Also acts as a histone glutaryltransferase : catalyzes glutarylation of histone H4 on 'Lys-91' (H4K91glu), a mark that destabilizes nucleosomes by promoting dissociation of the H2A-H2B dimers from nucleosomes (PubMed : 31542297).. (Microbial infection) In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes.

Sequence similarities

Belongs to the acetyltransferase family. GCN5 subfamily.

Post-translational modifications

Acetylated at Lys-549, inhibiting the protein acetyltransferase activity (PubMed:23142079). Deacetylation at Lys-549 by SIRT6 promotes phosphorylation at Ser-307 and Thr-735 and subsequent activation of the protein acetyltransferase activity, leading to acetylation and inactivation of PPARGC1A (PubMed:23142079).

Subcellular localisation

Nucleus