JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB113856

Recombinant Human GCSH protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human GCSH protein is a Human Full Length protein, in the 49 to 173 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

Lipoic acid-containing protein, GCSH

1 Images
SDS-PAGE - Recombinant Human GCSH protein (AB113856)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human GCSH protein (AB113856)

15% SDS-PAGE analysis of 3 μg ab113856.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

P23434

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMSVRKFTEKHEWVTTENGIGTVGISNFAQEALGDVVYCSLPEVGTKLNKQDEFGALESVKAASELYSPLSGEVTEINEALAENPGLVNKSCYEDGWLIKMTLSNPSELDELMSEEAYEKYIKSIEE","proteinLength":"Full Length","predictedMolecularWeight":"16.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":173,"aminoAcidStart":49,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P23434","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column
style
left-column

Specifications

Form

Liquid

Additional notes

ab113856 was purified using conventional chromatography techniques.

style
left-column

General info

Function

The glycine cleavage system catalyzes the degradation of glycine. The H protein (GCSH) shuttles the methylamine group of glycine from the P protein (GLDC) to the T protein (GCST). Has a pivotal role in the lipoylation of enzymes involved in cellular energetics such as the mitochondrial dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex (DLAT), and the mitochondrial dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex (DLST) (PubMed : 36190515).

Sequence similarities

Belongs to the GcvH family.

Subcellular localisation

Mitochondrion

style
left-column

Product protocols

style
left-column

Target data

The glycine cleavage system catalyzes the degradation of glycine. The H protein (GCSH) shuttles the methylamine group of glycine from the P protein (GLDC) to the T protein (GCST). Has a pivotal role in the lipoylation of enzymes involved in cellular energetics such as the mitochondrial dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex (DLAT), and the mitochondrial dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex (DLST) (PubMed : 36190515).
See full target information Glycine cleavage system H protein, mitochondrial
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB250083

Anti-SDSL antibody [EPR12192] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-SDSL antibody [EPR12192] - BSA and Azide free (AB250083)

  • 0
  • 0
Primary Antibodies

AB184247

Anti-DRP1 antibody [EPR19274]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-DRP1 antibody [EPR19274] (AB184247)

  • 5
  • 5
Primary Antibodies

AB124669

Anti-ARTS1/ERAP1 antibody [EPR6069]

RabMAb|Recombinant

Western blot - Anti-ARTS1/ERAP1 antibody [EPR6069] (AB124669)

  • 0
  • 0
Primary Antibodies

AB92355

Anti-ILF3 antibody [EPR3626]

RabMAb|Recombinant|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-ILF3 antibody [EPR3626] (AB92355)

  • 4
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com