JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB269223

Recombinant human GDF 5 protein (Active)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant human GDF 5 protein (Active) is a Human Full Length protein, in the 382 to 501 aa range, expressed in Escherichia coli, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.

View Alternative Names

BMP14, CDMP1, GDF5, Growth/differentiation factor 5, GDF-5, Bone morphogenetic protein 14, Cartilage-derived morphogenetic protein 1, Lipopolysaccharide-associated protein 4, Radotermin, BMP-14, CDMP-1, LAP-4, LPS-associated protein 4

1 Images
SDS-PAGE - Recombinant human GDF 5 protein (Active) (AB269223)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human GDF 5 protein (Active) (AB269223)

SDS-PAGE analysis of ab269223 at 1ug/lane under (-) non-reducing and (+) reducing conditions. 4-20% Tris glycine gel. Stained with coomassie blue.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Escherichia coli

Tags

Tag free

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Alkaline phosphatase activity in ATDC5 cells ≤1.2 ug/mL; ≥ 8.3 x 10^2 units/mg (typical ED50 is < 25 ng/mL).

Accession

P43026

Animal free

No

Carrier free

No

Species

Human

Reconstitution

Reconstitute at 0.1 mg/mL in water

Storage buffer

Constituents: 0.1% Trifluoroacetic acid

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column
style
left-column

Sequence info

[{"sequence":"MAPLATRQGKRPSKNLKARCSRKALHVNFKDMGWDDWIIAPLEYEAFHCEGLCEFPLRSHLEPTNHAVIQTLMNSMDPESTPPTCCVPTRLSPISILFIDSANNVVYKQYEDMVVESCGCR","proteinLength":"Full Length","predictedMolecularWeight":"55 kDa","actualMolecularWeight":null,"aminoAcidEnd":501,"aminoAcidStart":382,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P43026","tags":[]}]
style
left-column

Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C.
True
style
left-column
style
left-column

Specifications

Form

Lyophilized

Additional notes

nan

style
left-column

General info

Function

Growth factor involved in bone and cartilage formation. During cartilage development regulates differentiation of chondrogenic tissue through two pathways. Firstly, positively regulates differentiation of chondrogenic tissue through its binding of high affinity with BMPR1B and of less affinity with BMPR1A, leading to induction of SMAD1-SMAD5-SMAD8 complex phosphorylation and then SMAD protein signaling transduction (PubMed : 15530414, PubMed : 21976273, PubMed : 24098149, PubMed : 25092592). Secondly, negatively regulates chondrogenic differentiation through its interaction with NOG (PubMed : 21976273). Required to prevent excessive muscle loss upon denervation. This function requires SMAD4 and is mediated by phosphorylated SMAD1/5/8 (By similarity). Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes (PubMed : 11276205).

Sequence similarities

Belongs to the TGF-beta family.

style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Growth factor involved in bone and cartilage formation. During cartilage development regulates differentiation of chondrogenic tissue through two pathways. Firstly, positively regulates differentiation of chondrogenic tissue through its binding of high affinity with BMPR1B and of less affinity with BMPR1A, leading to induction of SMAD1-SMAD5-SMAD8 complex phosphorylation and then SMAD protein signaling transduction (PubMed : 15530414, PubMed : 21976273, PubMed : 24098149, PubMed : 25092592). Secondly, negatively regulates chondrogenic differentiation through its interaction with NOG (PubMed : 21976273). Required to prevent excessive muscle loss upon denervation. This function requires SMAD4 and is mediated by phosphorylated SMAD1/5/8 (By similarity). Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes (PubMed : 11276205).
See full target information GDF5
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column
style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com