Recombinant human GDF11 protein (Active) is a Human Full Length protein, in the 299 to 407 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
N L G L D C D E H S S E S R C C R Y P L T V D F E A F G W D W I I A P K R Y K A N Y C S G Q C E Y M F M Q K Y P H T H L V Q Q A N P R G S A G P C C T P T K M S P I N M L Y F N D K Q Q I I Y G K I P G M V V D R C G C S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Secreted signal that acts globally to regulate anterior/posterior axial patterning during development. May play critical roles in patterning both mesodermal and neural tissues (By similarity). It is required for proper vertebral patterning and orofacial development (PubMed:31215115). Signals through activin receptors type-2, ACVR2A and ACVR2B, and activin receptors type-1, ACVR1B, ACVR1C and TGFBR1 leading to the phosphorylation of SMAD2 and SMAD3 (PubMed:28257634).
Alternative names
BMP11, GDF11, Growth/differentiation factor 11, GDF-11, Bone morphogenetic protein 11, BMP-11
Recommended products
Recombinant human GDF11 protein (Active) is a Human Full Length protein, in the 299 to 407 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Alkaline phosphatase activity induced in ATDC-5 cells.
Acceptance Criteria: ED50 ≤100 ng/mL(≥ 1.0 x 104 units/mg).
- Accession
- O95390-1
- Animal free
- No
- Species
- Human
- Reconstitution
- Reconstitute at 0.1 mg/mL in 10 mM HCl
- Concentration
- Storage buffer
Constituents: 0.1% Trifluoroacetic acid
Sequence info
Amino acid sequence
- N L G L D C D E H S S E S R C C R Y P L T V D F E A F G W D W I I A P K R Y K A N Y C S G Q C E Y M F M Q K Y P H T H L V Q Q A N P R G S A G P C C T P T K M S P I N M L Y F N D K Q Q I I Y G K I P G M V V D R C G C S
- Accession
- O95390
- Protein length
- Full Length
- Predicted molecular weight
- 12.5 kDa
- Amino acids
- 299 to 407
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
General info
- Function
Secreted signal that acts globally to regulate anterior/posterior axial patterning during development. May play critical roles in patterning both mesodermal and neural tissues (By similarity). It is required for proper vertebral patterning and orofacial development (PubMed:31215115). Signals through activin receptors type-2, ACVR2A and ACVR2B, and activin receptors type-1, ACVR1B, ACVR1C and TGFBR1 leading to the phosphorylation of SMAD2 and SMAD3 (PubMed:28257634).
- Sequence similarities
Belongs to the TGF-beta family.
- Post-translational modifications
Synthesized as large precursor molecule that undergoes proteolytic cleavage by furin-like proteases (PubMed:31215115). This produces an inactive form consisting of the mature C-terminal portion non-covalently bound to its cleaved N-terminal propeptide. Activation of the mature form requires additional cleavage of the propeptide by a tolloid-like metalloproteinase.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
This product shares 100% homology with amino acids 297-405 of Rat and Mouse GDF11.
This product was previously labelled as BMP11, BMP11 + GDF8 / Myostatin
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2 product images
Functional Studies - Recombinant human GDF11 protein (Active) (ab218080)
Functional analysis of ab218080
SDS-PAGE - Recombinant human GDF11 protein (Active) (ab218080)
SDS-PAGE analysis of ab218080 at 1 μg under (1) non-reducing and (2) reducing conditions in a 4-20% Tris-Glycine gel, stained with Coomassie Blue. Human GDF11 is a homodimer with a total; predicted MW of 24.9 kDa (each monomer 12.4 kDa).
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