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AB218103

Recombinant human GDF11 protein (Animal Free)

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Recombinant human GDF11 protein (Animal Free) is a Human Full Length protein, in the 299 to 407 aa range, expressed in Escherichia coli, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, HPLC.

View Alternative Names

BMP11, GDF11, Growth/differentiation factor 11, GDF-11, Bone morphogenetic protein 11, BMP-11

2 Images
Functional Studies - Recombinant human GDF11 protein (Animal Free) (AB218103)
  • FuncS

Unknown

Functional Studies - Recombinant human GDF11 protein (Animal Free) (AB218103)

Functional analysis of ab218103

SDS-PAGE - Recombinant human GDF11 protein (Animal Free) (AB218103)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human GDF11 protein (Animal Free) (AB218103)

SDS-PAGE analysis of ab218103 (1μg) stained with Coomassie blue :

Lane 1 : Non-reduced

Lane 2 : Reduced

4-20% Tris-Glycine gel

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, HPLC, FuncS

applications

Biologically active

Yes

Biological activity

Alkaline phosphatase activity in ATDC5 cells: ED50 ≤100 ng/ml; ≥ 1.0 x 104 units/mg (typical ED50 is < 10 ng/mL).

Accession

O95390

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

Reconstitute in 10 mM HCl

Storage buffer

Constituents: 0.1% Trifluoroacetic acid

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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AB267609

Human GDF11 ELISA Kit

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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

This product shares 100% homology with amino acids 297-405 of Rat and Mouse GDF11.

This product was previously labelled as BMP11, BMP11 + GDF8 / Myostatin

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Sequence info

[{"sequence":"NLGLDCDEHSSESRCCRYPLTVDFEAFGWDWIIAPKRYKANYCSGQCEYMFMQKYPHTHLVQQANPRGSAGPCCTPTKMSPINMLYFNDKQQIIYGKIPGMVVDRCGCS","proteinLength":"Full Length","predictedMolecularWeight":"12.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":407,"aminoAcidStart":299,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O95390","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
True
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Specifications

Form

Lyophilized

Additional notes

Reducing and non-reducing SDS-PAGE.

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General info

Function

Secreted signal that acts globally to regulate anterior/posterior axial patterning during development. May play critical roles in patterning both mesodermal and neural tissues (By similarity). It is required for proper vertebral patterning and orofacial development (PubMed : 31215115). Signals through activin receptors type-2, ACVR2A and ACVR2B, and activin receptors type-1, ACVR1B, ACVR1C and TGFBR1 leading to the phosphorylation of SMAD2 and SMAD3 (PubMed : 28257634).

Sequence similarities

Belongs to the TGF-beta family.

Post-translational modifications

Synthesized as large precursor molecule that undergoes proteolytic cleavage by furin-like proteases (PubMed:31215115). This produces an inactive form consisting of the mature C-terminal portion non-covalently bound to its cleaved N-terminal propeptide. Activation of the mature form requires additional cleavage of the propeptide by a tolloid-like metalloproteinase.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Secreted signal that acts globally to regulate anterior/posterior axial patterning during development. May play critical roles in patterning both mesodermal and neural tissues (By similarity). It is required for proper vertebral patterning and orofacial development (PubMed : 31215115). Signals through activin receptors type-2, ACVR2A and ACVR2B, and activin receptors type-1, ACVR1B, ACVR1C and TGFBR1 leading to the phosphorylation of SMAD2 and SMAD3 (PubMed : 28257634).
See full target information GDF11
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Product
Proteins & Peptides

AB218080

Recombinant human GDF11 protein (Active)

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Alternative Product
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AB202626

Recombinant Human GDF11 protein (denatured)

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recombinant-human-gdf11-protein-denatured-ab202626

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