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AB218103

Recombinant human GDF11 protein (Animal Free)

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Recombinant human GDF11 protein (Animal Free) is a Human Full Length protein, in the 299 to 407 aa range, expressed in Escherichia coli, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, HPLC.

View Alternative Names

BMP11, GDF11, Growth/differentiation factor 11, GDF-11, Bone morphogenetic protein 11, BMP-11

2 Images
Functional Studies - Recombinant human GDF11 protein (Animal Free) (AB218103)
  • FuncS

Unknown

Functional Studies - Recombinant human GDF11 protein (Animal Free) (AB218103)

Functional analysis of ab218103

SDS-PAGE - Recombinant human GDF11 protein (Animal Free) (AB218103)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human GDF11 protein (Animal Free) (AB218103)

SDS-PAGE analysis of ab218103 (1μg) stained with Coomassie blue :

Lane 1 : Non-reduced

Lane 2 : Reduced

4-20% Tris-Glycine gel

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Escherichia coli

Tags

Tag free

Applications

HPLC, FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Alkaline phosphatase activity in ATDC5 cells: ED50 ≤100 ng/ml; ≥ 1.0 x 104 units/mg (typical ED50 is < 10 ng/mL).

Accession

O95390

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

Reconstitute in 10 mM HCl

Storage buffer

Constituents: 0.1% Trifluoroacetic acid

storage-buffer

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Secondary Antibodies

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  • 5
  • 20
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product shares 100% homology with amino acids 297-405 of Rat and Mouse GDF11.

This product was previously labelled as BMP11, BMP11 + GDF8 / Myostatin

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Sequence info

[{"sequence":"NLGLDCDEHSSESRCCRYPLTVDFEAFGWDWIIAPKRYKANYCSGQCEYMFMQKYPHTHLVQQANPRGSAGPCCTPTKMSPINMLYFNDKQQIIYGKIPGMVVDRCGCS","proteinLength":"Full Length","predictedMolecularWeight":"12.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":407,"aminoAcidStart":299,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O95390","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
True
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Specifications

Form

Lyophilized

Additional notes

Reducing and non-reducing SDS-PAGE.

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General info

Function

Secreted signal that acts globally to regulate anterior/posterior axial patterning during development. May play critical roles in patterning both mesodermal and neural tissues (By similarity). It is required for proper vertebral patterning and orofacial development (PubMed : 31215115). Signals through activin receptors type-2, ACVR2A and ACVR2B, and activin receptors type-1, ACVR1B, ACVR1C and TGFBR1 leading to the phosphorylation of SMAD2 and SMAD3 (PubMed : 28257634).

Sequence similarities

Belongs to the TGF-beta family.

Post-translational modifications

Synthesized as large precursor molecule that undergoes proteolytic cleavage by furin-like proteases (PubMed:31215115). This produces an inactive form consisting of the mature C-terminal portion non-covalently bound to its cleaved N-terminal propeptide. Activation of the mature form requires additional cleavage of the propeptide by a tolloid-like metalloproteinase.

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Product protocols

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Target data

Secreted signal that acts globally to regulate anterior/posterior axial patterning during development. May play critical roles in patterning both mesodermal and neural tissues (By similarity). It is required for proper vertebral patterning and orofacial development (PubMed : 31215115). Signals through activin receptors type-2, ACVR2A and ACVR2B, and activin receptors type-1, ACVR1B, ACVR1C and TGFBR1 leading to the phosphorylation of SMAD2 and SMAD3 (PubMed : 28257634).
See full target information GDF11
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Product promise

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