JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB203723

Recombinant Human GDF8 / Myostatin protein

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human GDF8 / Myostatin protein is a Human Full Length protein, in the 24 to 375 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, WB, Mass Spec.

View Alternative Names

GDF8, MSTN, Growth/differentiation factor 8, GDF-8, Myostatin

1 Images
SDS-PAGE - Recombinant Human GDF8 / Myostatin protein (AB203723)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human GDF8 / Myostatin protein (AB203723)

14 % SDS-PAGE analysis of ab203723 :
1. Reduced and boiled sample, 2.5 μg/lane.
2. Non-reduced and non-boiled sample, 2.5 μg/lane.

The approx. 50 kDa band represents the complex of Myostatin-Propeptide. The band corresponding to approx. 38 kDa represents cleaved form of Propeptide and similarly the approx. 12 kDa band belongs to cleaved Myostatin domain. Myostatin domains makes dimmers in non-reducing conditions resulting in bends of approx. 25 and 97 kDa, respectively.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

HEK 293 cells

Tags

His tag N-Terminus

Applications

WB, Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

O14793

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

storage-buffer

style
left-column

Complementary Products

Reagents, Controls & Accessories

AB116028

Prestained Protein Ladder - Broad molecular weight (10 - 245 kDa)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (10 - 245 kDa) (AB116028)

  • 5
  • 3
Proteins & Peptides

AB218103

Recombinant human GDF11 protein (Animal Free)

Bioactive

Functional Studies - Recombinant human GDF11 protein (Animal Free) (AB218103)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Proteins & Peptides

AB259381

Recombinant human IL-6 protein (Active)

Premium Bioactive

Functional Studies (Neut/Block) - Recombinant human IL-6 protein (Active) (AB259381)

  • 5
  • 1
Primary Antibodies

AB203076

Anti-GDF8 / Myostatin antibody

Western blot - Anti-GDF8 / Myostatin antibody (AB203076)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Frozen from a 0.25–0.5 mg/ml solution. Defrost at ambient temperature. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.
style
left-column

Sequence info

[{"sequence":"HHHHHHASNENSEQKENVEKEGLCNACTWRQNTKSSRIEAIKIQILSKLRLETAPNISKDVIRQLLPKAPPLRELIDQYDVQRDDSSDGSLEDDDYHATTETIITMPTESDFLMQVDGKPKCCFFKFSSKIQYNKVVKAQLWIYLRPVETPTTVFVQILRLIKPMKDGTRYTGIRSLKLDMNPGTGIWQSIDVKTVLQNWLKQPESNLGIEIKALDENGHDLAVTFPGPGEDGLNPFLEVKVTDTPKRSRRDFGLDCDEHSTESRCCRYPLTVDFEAFGWDWIIAPKRYKANYCSGECEFVFLQKYPHTHLVHQANPRGSAGPCCTPTKMSPINMLYFNGKEQIIYGKIPAMVVDRCGCS","proteinLength":"Full Length","predictedMolecularWeight":"71.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":375,"aminoAcidStart":24,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"O14793","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
False
style
left-column
style
left-column

Specifications

Form

Liquid

Additional notes

Assessed by densitometry. Purifed using Ni-NTA chromatography.

style
left-column

General info

Function

Acts specifically as a negative regulator of skeletal muscle growth.

Sequence similarities

Belongs to the TGF-beta family.

Post-translational modifications

Synthesized as large precursor molecule that undergoes proteolytic cleavage to generate an N-terminal propeptide and a disulfide linked C-terminal dimer, which is the biologically active molecule. The circulating form consists of a latent complex of the C-terminal dimer and other proteins, including its propeptide, which maintain the C-terminal dimer in a latent, inactive state. Ligand activation requires additional cleavage of the prodomain by a tolloid-like metalloproteinase.

style
left-column

Product protocols

style
left-column

Target data

Acts specifically as a negative regulator of skeletal muscle growth.
See full target information MSTN
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com