Recombinant human GDF8 / Myostatin protein (Animal Free) is a Human Full Length protein, in the 267 to 375 aa range, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
D F G L D C D E H S T E S R C C R Y P L T V D F E A F G W D W I I A P K R Y K A N Y C S G E C E F V F L Q K Y P H T H L V H Q A N P R G S A G P C C T P T K M S P I N M L Y F N G K E Q I I Y G K I P A M V V D R C G C S
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Acts specifically as a negative regulator of skeletal muscle growth.
Alternative names
GDF8, MSTN, Growth/differentiation factor 8, GDF-8, Myostatin
Recommended products
Recombinant human GDF8 / Myostatin protein (Animal Free) is a Human Full Length protein, in the 267 to 375 aa range, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- MPC-11 cell cytotoxicity. ≤50 ng/ml; ≥ 2.0 x 104 units/mg.
- Accession
- O14793-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute at 0.1 mg/mL in 20 mM HCl
- Concentration
- Storage buffer
Constituents: 0.1% Trifluoroacetic acid
Sequence info
Amino acid sequence
- D F G L D C D E H S T E S R C C R Y P L T V D F E A F G W D W I I A P K R Y K A N Y C S G E C E F V F L Q K Y P H T H L V H Q A N P R G S A G P C C T P T K M S P I N M L Y F N G K E Q I I Y G K I P A M V V D R C G C S
- Accession
- O14793
- Protein length
- Full Length
- Predicted molecular weight
- 12.4 kDa
- Amino acids
- 267 to 375
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
General info
- Function
Acts specifically as a negative regulator of skeletal muscle growth.
- Sequence similarities
Belongs to the TGF-beta family.
- Post-translational modifications
Synthesized as large precursor molecule that undergoes proteolytic cleavage to generate an N-terminal propeptide and a disulfide linked C-terminal dimer, which is the biologically active molecule. The circulating form consists of a latent complex of the C-terminal dimer and other proteins, including its propeptide, which maintain the C-terminal dimer in a latent, inactive state. Ligand activation requires additional cleavage of the prodomain by a tolloid-like metalloproteinase.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Peptide Array - Recombinant human GDF8 / Myostatin protein (Animal Free) (ab256090)
SDS-PAGE analysis of ab256090 (1 μg) under non-reducing (Lane 1) and reducing (Lane 2) conditions. ab256090 is a homodimer with a predicted MW of 24.8 kDa (monomer size is 12.4 kDa).
4-20% Tris-Glycine gel. Coomassie Blue staining.
Functional Studies - Recombinant human GDF8 / Myostatin protein (Animal Free) (ab256090)
Functional analysis of ab256090.
Downloads
Product protocols
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