Recombinant human GDNF protein (Active) is a Human Full Length protein in the 76 to 211 aa range with ≥95% purity, ≤ 0.005 EU/µg endotoxin level and suitable for Cell Culture, Functional studies, mass spectrometry and SDS-PAGE. The predicted molecular weight of ab259417 protein is 15.4 kDa.
- Save time and ensure accurate results - use our recombinant human GDNF protein as a control
- Optimal protein bioactivity, stability and reproducibility
- Available in different sizes to fit your experimental needs
Images
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, HPLC, Cell Culture
- Biologically active
- Yes
Amino acid sequence
K R S P D K Q M A V L P R R E R N R Q A A A A N P E N S R G K G R R G Q R G K N R G C V L T A I H L N V T D L G L G Y E T K E E L I F R Y C S G S C D A A E T T Y D K I L K N L S R N R R L V S D K V G Q A C C R P I A F D D D L S F L D D N L V Y H I L R K H S A K R C G C I
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application Cell Culture | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake (PubMed:8493557). Acts by binding to its coreceptor, GFRA1, leading to autophosphorylation and activation of the RET receptor (PubMed:10829012, PubMed:25242331, PubMed:31535977). Involved in the development of the neural crest (PubMed:15242795).
Alternative names
Glial cell line-derived neurotrophic factor, hGDNF, Astrocyte-derived trophic factor, ATF, GDNF
Recommended products
Recombinant human GDNF protein (Active) is a Human Full Length protein in the 76 to 211 aa range with ≥95% purity, ≤ 0.005 EU/µg endotoxin level and suitable for Cell Culture, Functional studies, mass spectrometry and SDS-PAGE. The predicted molecular weight of ab259417 protein is 15.4 kDa.
- Save time and ensure accurate results - use our recombinant human GDNF protein as a control
- Optimal protein bioactivity, stability and reproducibility
- Available in different sizes to fit your experimental needs
Key facts
- Purity
- >=95% SDS-PAGE
- Endotoxin level
- <= 0.005 EU/µg
- Expression system
- HEK 293 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS, MS, HPLC, Cell Culture
- Biologically active
- Yes
- Biological activity
- Fully biologically active determined by the dose dependent increase in proliferation of SHSY-5Y cells in the presence of GFRα.
ED50 is ≤9.55 ng/ml, coresponding to a specific activity of 1.05 x 105 units/mg.
- Accession
- P39905-1
- Animal free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute in PBS
- Concentration
- Storage buffer
pH: 6 - 8
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Sequence info
Amino acid sequence
- K R S P D K Q M A V L P R R E R N R Q A A A A N P E N S R G K G R R G Q R G K N R G C V L T A I H L N V T D L G L G Y E T K E E L I F R Y C S G S C D A A E T T Y D K I L K N L S R N R R L V S D K V G Q A C C R P I A F D D D L S F L D D N L V Y H I L R K H S A K R C G C I
- Accession
- P39905
- Protein length
- Full Length
- Predicted molecular weight
- 15.4 kDa
- Actual molecular weight
- 15.41 kDa
- Amino acids
- 76 to 211
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
- Additional notes
=95% by HPLC
General info
- Function
Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake (PubMed:8493557). Acts by binding to its coreceptor, GFRA1, leading to autophosphorylation and activation of the RET receptor (PubMed:10829012, PubMed:25242331, PubMed:31535977). Involved in the development of the neural crest (PubMed:15242795).
- Sequence similarities
Belongs to the TGF-beta family. GDNF subfamily.
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Ensure the validity of your result using our recombinant human GDNF protein ab259417 as a control.
The ab259417 GDNF protein is sourced from HEK293 cells and can be used as a positive control in SDS-PAGE, mass spectrometry and HPLC.
Check out our protein gel staining guide for SDS-PAGE here
Premium Bioactive Protein range
The ab259417 GDNF protein is part of the premium bioactive protein range which are ideal for preclinical cell culture and functional studies. These recombinant proteins are of the highest activity, purity, and consistency, meeting rigorous biophysical characterization.
More premium bioactive proteins can be found here
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Functional Studies - Recombinant human GDNF protein (Active) (ab259417)
Fully biologically active determined by the dose dependent increase in proliferation of SHSY-5Y cells in the presence of GFRα.
ED50 is ≤9.55 ng/ml, coresponding to a specific activity of 1.05 x 105 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot: GR3368300-1
SDS-PAGE - Recombinant human GDNF protein (Active) (ab259417)
SDS-PAGE analysis of ab259417.
Mass Spectrometry - Recombinant human GDNF protein (Active) (ab259417)
Mass determination by ESI TOF.
Predicted mass is 15409.58 +/- 10Da by ESI TOF. Observed mass is 15411 Da.
HPLC - Recombinant human GDNF protein (Active) (ab259417)
HPLC analysis of ab259417.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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