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Recombinant human GDNF protein (Active) is a Human Full Length protein, in the 76 to 211 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC, Cell Culture.
Alternative names=Glial cell line-derived neurotrophic factor, hGDNF, Astrocyte-derived trophic factor, ATF, GDNF
- Purity
>=95% SDS-PAGE
- Endotoxin level
<= 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE, FuncS, MS, HPLC, Cell Culture
- Biologically active
Yes
- Actual molecular weight
15.41 kDa
- Sequence
K R S P D K Q M A V L P R R E R N R Q A A A A N P E N S R G K G R R G Q R G K N R G C V L T A I H L N V T D L G L G Y E T K E E L I F R Y C S G S C D A A E T T Y D K I L K N L S R N R R L V S D K V G Q A C C R P I A F D D D L S F L D D N L V Y H I L R K H S A K R C G C I
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application Cell Culture | Reactivity Reacts | Dilution info - | Notes - |
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Recombinant human GDNF protein (Active) is a Human Full Length protein, in the 76 to 211 aa range, expressed in HEK 293, with >=95% purity, <= 0.005 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS, MS, HPLC, Cell Culture.
Alternative names=Glial cell line-derived neurotrophic factor, hGDNF, Astrocyte-derived trophic factor, ATF, GDNF
Key facts
- Purity
>=95% SDS-PAGE
- Endotoxin level
<= 0.005 EU/µg
- Expression system
HEK 293 cells
- Tags
Tag free
- Applications
SDS-PAGE, FuncS, MS, HPLC, Cell Culture
- Biologically active
Yes
- Biological activity
Fully biologically active determined by the dose dependent increase in proliferation of SHSY-5Y cells in the presence of GFRα.
ED50 is ≤9.55 ng/ml, coresponding to a specific activity of 1.05 x 105 units/mg.
- Predicted molecular weight
15.4 kDa
- Actual molecular weight
15.41 kDa
- Amino acids
76 to 211
- Sequence
K R S P D K Q M A V L P R R E R N R Q A A A A N P E N S R G K G R R G Q R G K N R G C V L T A I H L N V T D L G L G Y E T K E E L I F R Y C S G S C D A A E T T Y D K I L K N L S R N R R L V S D K V G Q A C C R P I A F D D D L S F L D D N L V Y H I L R K H S A K R C G C I
- Accession
- P39905-1
- Protein length
Full Length
- Animal free
Yes
- Nature
Recombinant
- Species
Human
- Reconstitution
Reconstitute with phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw.Lyophilized contents may appear as either a translucent film or a white power. This variance does not affect the quality of the product. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
- Concentration
- Storage buffer
pH: 6 - 8
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic
Specifications
- Form
Lyophilized
- Additional notes
=95% by HPLC
General info
- Function
Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
- Sequence similarities
Belongs to the TGF-beta family. GDNF subfamily.
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate long-term storage conditions
Ambient
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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