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AB259417

Recombinant human GDNF protein (Active)

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Recombinant human GDNF protein (Active) is a Human Full Length protein in the 76 to 211 aa range with ≥95% purity, ≤ 0.005 EU/µg endotoxin level and suitable for Cell Culture, Functional studies, mass spectrometry and SDS-PAGE. The predicted molecular weight of ab259417 protein is 15.4 kDa.

- Save time and ensure accurate results - use our recombinant human GDNF protein as a control
- Optimal protein bioactivity, stability and reproducibility
- Available in different sizes to fit your experimental needs

View Alternative Names

Glial cell line-derived neurotrophic factor, hGDNF, Astrocyte-derived trophic factor, ATF, GDNF

4 Images
Mass Spectrometry - Recombinant human GDNF protein (Active) (AB259417)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant human GDNF protein (Active) (AB259417)

Mass determination by ESI TOF.

Predicted mass is 15409.58 +/- 10Da by ESI TOF. Observed mass is 15411 Da.

Functional Studies - Recombinant human GDNF protein (Active) (AB259417)
  • FuncS

Supplier Data

Functional Studies - Recombinant human GDNF protein (Active) (AB259417)

Fully biologically active determined by the dose dependent increase in proliferation of SHSY-5Y cells in the presence of GFRα.

ED50 is ≤9.55 ng/ml, coresponding to a specific activity of 1.05 x 105 units/mg.

Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.

Lot : GR3368300-1

HPLC - Recombinant human GDNF protein (Active) (AB259417)
  • HPLC

Supplier Data

HPLC - Recombinant human GDNF protein (Active) (AB259417)

HPLC analysis of ab259417.

SDS-PAGE - Recombinant human GDNF protein (Active) (AB259417)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human GDNF protein (Active) (AB259417)

SDS-PAGE analysis of ab259417.

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

<0.005 EU/µg

Expression system

HEK 293 cells

Tags

Tag free

Applications

SDS-PAGE, Cell Culture, FuncS, HPLC, Mass Spec

applications

Biologically active

Yes

Biological activity

Fully biologically active determined by the dose dependent increase in proliferation of SHSY-5Y cells in the presence of GFRα.

ED50 is ≤9.55 ng/ml, coresponding to a specific activity of 1.05 x 105 units/mg.

Accession

P39905

Animal free

Yes

Carrier free

No

Species

Human

Reconstitution

Reconstitute in PBS

Storage buffer

pH: 6 - 8 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Cell Culture": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Ensure the validity of your result using our recombinant human GDNF protein ab259417 as a control.

The ab259417 GDNF protein is sourced from HEK293 cells and can be used as a positive control in SDS-PAGE, mass spectrometry and HPLC.

Check out our protein gel staining guide for SDS-PAGE here

Premium Bioactive Protein range

The ab259417 GDNF protein is part of the premium bioactive protein range which are ideal for preclinical cell culture and functional studies. These recombinant proteins are of the highest activity, purity, and consistency, meeting rigorous biophysical characterization.

More premium bioactive proteins can be found here
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Sequence info

[{"sequence":"KRSPDKQMAVLPRRERNRQAAAANPENSRGKGRRGQRGKNRGCVLTAIHLNVTDLGLGYETKEELIFRYCSGSCDAAETTYDKILKNLSRNRRLVSDKVGQACCRPIAFDDDLSFLDDNLVYHILRKHSAKRCGCI","proteinLength":"Full Length","predictedMolecularWeight":"15.4 kDa","actualMolecularWeight":"15.41 kDa","aminoAcidEnd":211,"aminoAcidStart":76,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P39905","tags":[]}]
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
True
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Specifications

Form

Lyophilized

Additional notes

=95%  by HPLC

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General info

Function

Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake (PubMed : 8493557). Acts by binding to its coreceptor, GFRA1, leading to autophosphorylation and activation of the RET receptor (PubMed : 10829012, PubMed : 25242331, PubMed : 31535977). Involved in the development of the neural crest (PubMed : 15242795).

Sequence similarities

Belongs to the TGF-beta family. GDNF subfamily.

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Product protocols

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Target data

Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake (PubMed : 8493557). Acts by binding to its coreceptor, GFRA1, leading to autophosphorylation and activation of the RET receptor (PubMed : 10829012, PubMed : 25242331, PubMed : 31535977). Involved in the development of the neural crest (PubMed : 15242795).
See full target information GDNF
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com