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AB203536

Recombinant Human Gelsolin protein

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Recombinant Human Gelsolin protein is a Human Full Length protein, in the 3 to 731 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

Gelsolin, AGEL, Actin-depolymerizing factor, Brevin, ADF, GSN

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

T7 tag N-Terminus His tag N-Terminus TEV tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P06396

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.32% Tris HCl

storage-buffer

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Complementary Products

Reagents, Controls & Accessories

AB133411

Low Fluorescence Western Membrane (PVDF)

  • 5
  • 1
Assay Kits

AB270215

Human Gelsolin ELISA Kit

Recombinant|SimpleStep

ELISA - Human Gelsolin ELISA Kit (AB270215)

  • 5
  • 1
Primary Antibodies

AB109014

Anti-Gelsolin antibody [EPR1942]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gelsolin antibody [EPR1942] (AB109014)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Assay Kits

AB253312

Human Gelsolin Antibody Pair - BSA and Azide free

RabMAb|Recombinant

Sandwich ELISA - Human Gelsolin Antibody Pair - BSA and Azide free (AB253312)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MASMTGGQQMGRGHHHHHHENLYFQGGEFDVEHPEFLKAGKEPGLQIWRVEKFDLVPVPTNLYGDFFTGDAYVILKTVQLRNGNLQYDLHYWLGNECSQDESGAAAIFTVQLDDYLNGRAVQHREVQGFESATFLGYFKSGLKYKKGGVASGFKHVVPNEVVVQRLFQVKGRRVVRATEVPVSWESFNNGDCFILDLGNNIHQWCGSNSNRYERLKATQVSKGIRDNERSGRARVHVSEEGTEPEAMLQVLGPKPALPAGTEDTAKEDAANRKLAKLYKVSNGAGTMSVSLVADENPFAQGALKSEDCFILDHGKDGKIFVWKGKQANTEERKAALKTASDFITKMDYPKQTQVSVLPEGGETPLFKQFFKNWRDPDQTDGLGLSYLSSHIANVERVPFDAATLHTSTAMAAQHGMDDDGTGQKQIWRIEGSNKVPVDPATYGQFYGGDSYIILYNYRHGGRQGQIIYNWQGAQSTQDEVAASAILTAQLDEELGGTPVQSRVVQGKEPAHLMSLFGGKPMIIYKGGTSREGGQTAPASTRLFQVRANSAGATRAVEVLPKAGALNSNDAFVLKTPSAAYLWVGTGASEAEKTGAQELLRVLRAQPVQVAEGSEPDGFWEALGGKAAYRTSPRLKDKKMDAHPPRLFACSNKIGRFVIEEVPGELMQEDLATDDVMLLDTWDQVFVWVGKDSQEEEKTEALTSAKRYIETDPANRDRRTPITVVKQGFEPPSFVGWFLGWDDDYWSVDPLDRAMAELAA","proteinLength":"Full Length","predictedMolecularWeight":"84 kDa","actualMolecularWeight":null,"aminoAcidEnd":731,"aminoAcidStart":3,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P06396","tags":[{"tag":"T7","terminus":"N-Terminus"},{"tag":"His","terminus":"N-Terminus"},{"tag":"TEV","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gelsolin is a multifunctional actin-binding protein that exists in two main isoforms: cytoplasmic and plasma. It has a molecular mass of approximately 82 kDa. Gelsolin is expressed in a wide range of tissues including muscle epithelial and nerve cells. The protein's main mechanical function involves severing actin filaments which helps to regulate actin polymerization and depolymerization. Researchers sometimes refer to gelsolin as 'GSN'. The presence of the protein helps maintain the dynamic organization of the cytoskeleton by interacting with actin filaments.
Biological function summary

This protein acts in cell movement apoptosis and signal transduction. Gelsolin contributes to the remodeling of the actin cytoskeleton by promoting the disassembly of actin filaments allowing cells to change shape and move. Although it operates independently gelsolin’s activity can be influenced by calcium ions and phosphoinositides leading to its participation in various cellular activities. Gelsolin plays a role in platelet activation and may regulate cellular responses through association with the cytoskeletal complex.

Pathways

Gelsolin participates in the regulation of the actin cytoskeleton and apoptotic pathways. Within these pathways gelsolin interacts with proteins like cofilin to modulate actin filament dynamics. In apoptotic pathways it collaborates by altering the cytoskeletal architecture impacting cell death mechanisms. Gelsolin modifications in these pathways have implications for cellular processes like cell migration and apoptosis bringing it into relation with proteins such as caspases.

Gelsolin is linked to conditions such as gelsolin amyloidosis and cancer metastasis. Gelsolin amyloidosis results from mutations in the gelsolin gene which lead to the formation of amyloid fibrils causing dysfunction in nerves eyes and skin. In cancer altered gelsolin expression can influence tumor progression and metastasis through its role in cell motility. Related proteins such as actin and caspases could also play a part in these conditions either through direct interaction or through pathways in which gelsolin functions.
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Specifications

Form

Liquid

Additional notes

ab203536 was refolded using temperature shift inclusion body refolding technology and chromatographically purified.

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General info

Function

Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed (PubMed : 19666512). Plays a role in ciliogenesis (PubMed : 20393563).

Sequence similarities

Belongs to the villin/gelsolin family.

Post-translational modifications

Phosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids.

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Product protocols

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Target data

Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed (PubMed : 19666512). Plays a role in ciliogenesis (PubMed : 20393563).
See full target information GSN
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