Recombinant Human GFAP protein is a Human Full Length protein, in the 1 to 432 aa range, expressed in Wheat germ and suitable for SDS-PAGE, western blot and ELISA. The predicted molecular weight of ab114149 protein is 76 kDa.
- Save time and ensure accurate results - use our recombinant Glial fibrillary acidic protein (GFAP) as a control
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Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab114149 can be used as a WB positive control in conjunction with ab48050. |
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GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Glial fibrillary acidic protein, GFAP
Recombinant Human GFAP protein is a Human Full Length protein, in the 1 to 432 aa range, expressed in Wheat germ and suitable for SDS-PAGE, western blot and ELISA. The predicted molecular weight of ab114149 protein is 76 kDa.
- Save time and ensure accurate results - use our recombinant Glial fibrillary acidic protein (GFAP) as a control
pH: 8
Constituents: 0.79% Tris HCl, 0.3% Glutathione
Glutathione Sepharose 4 Fast Flow
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Belongs to the intermediate filament family.
Phosphorylated by PKN1.
Ensure the validity of your result using our recombinant human GFAP protein ab114149 as a positive control in western blot and SDS-PAGE.
Analyze your GFAP ELISA data using the ab114149 protein to generate and plot a standard curve.
Check out our protein gel staining guide for SDS-PAGE here
Check out of western blot protocol for more information here
Check out our ELISA protocol for more information here.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
12.5% SDS-PAGE analysis of ab114149, stained with Coomassie Blue.
All lanes: Anti-GFAP antibody - Astrocyte Marker (ab48050) at 1 µg/mL
All lanes: Western blot - Recombinant Human GFAP protein (ab114149) at 0.1 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10s
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