Recombinant Human GFAP protein is a Human Full Length protein, in the 1 to 432 aa range, expressed in Wheat germ and suitable for SDS-PAGE, western blot and ELISA. The predicted molecular weight of ab114149 protein is 76 kDa.
- Save time and ensure accurate results - use our recombinant Glial fibrillary acidic protein (GFAP) as a control
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Publications
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- Medicine 102:e328852023The neuroprotective effect of electro-acupuncture on cognitive recovery for patients with mild traumatic brain injury: A randomized controlled clinical trial.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaokun Jia,Yonghan Chen,Yi Wang,Linwei Jia,Yaohui Tian,Hao JiangPubMed 36820591
- Lupus science & medicine 9:2022Role of autoantibodies and blood-brain barrier leakage in cognitive impairment in systemic lupus erythematosus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJohn G Hanly,Alexandra Legge,Lyna Kamintsky,Alon Friedman,Javeria A Hashmi,Steven D Beyea,John Fisk,Antonina Omisade,Cynthia Calkin,Tim Bardouille,Chris Bowen,Kara Matheson,Marvin J FritzlerPubMed 35705307
- Journal of neural transmission (Vienna, Austria : 1996) 129:545-5552022Plasma autoantibodies to glial fibrillary acidic protein (GFAP) react with brain areas according to Braak staging of Parkinson's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEva Gschmack,Camelia-Maria Monoranu,Hecham Marouf,Sarah Meyer,Lena Lessel,Raja Idris,Daniela Berg,Walter Maetzler,Frank Steigerwald,Jens Volkmann,Manfred Gerlach,Peter Riederer,Eleni Koutsilieri,Carsten SchellerPubMed 35364741
- Nanoscale advances 4:1770-17782022Carbon dot-based fluorescent antibody nanoprobes as brain tumour glioblastoma diagnostics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMattia Ghirardello,Radhe Shyam,Xia Liu,Teodoro Garcia-Millan,Imke Sittel,Javier Ramos-Soriano,Kathreena M Kurian,M Carmen GalanPubMed 35434521
- Neuropsychiatric disease and treatment 16:2633-26472020Transcription Factor E2F1 Aggravates Neurological Injury in Ischemic Stroke via microRNA-122-Targeted Sprouty2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYunxia Wu et. al.PubMed 33177827
- Biosensors & bioelectronics 75:420-62015Microwave-accelerated bioassay technique for rapid and quantitative detection of biological and environmental samples.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMuzaffer Mohammed,Maleeha F Syed,Kadir AslanPubMed 26356762
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M E R R R I T S A A R R S Y V S S G E M M V G G L A P G R R L G P G T R L S L A R M P P P L P T R V D F S L A G A L N A G F K E T R A S E R A E M M E L N D R F A S Y I E K V R F L E Q Q N K A L A A E L N Q L R A K E P T K L A D V Y Q A E L R E L R L R L D Q L T A N S A R L E V E R D N L A Q D L A T V R Q K L Q D E T N L R L E A E N N L A A Y R Q E A D E A T L A R L D L E R K I E S L E E E I R F L R K I H E E E V R E L Q E Q L A R Q Q V H V E L D V A K P D L T A A L K E I R T Q Y E A M A S S N M H E A E E W Y R S K F A D L T D A A A R N A E L L R Q A K H E A N D Y R R Q L Q S L T C D L E S L R G T N E S L E R Q M R E Q E E R H V R E A A S Y Q E A L A R L E E E G Q S L K D E M A R H L Q E Y Q D L L N V K L A L D I E I A T Y R K L L E G E E N R I T I P V Q T F S N L Q I R E T S L D T K S V S E G H L K R N I V V K T V E M R D G E V I K E S K Q E H K D V M
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab114149 can be used as a WB positive control in conjunction with ab48050. |
Associated Products
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2 products for Alternative Product
Target data
Function
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Alternative names
Glial fibrillary acidic protein, GFAP
Recommended products
Recombinant Human GFAP protein is a Human Full Length protein, in the 1 to 432 aa range, expressed in Wheat germ and suitable for SDS-PAGE, western blot and ELISA. The predicted molecular weight of ab114149 protein is 76 kDa.
- Save time and ensure accurate results - use our recombinant Glial fibrillary acidic protein (GFAP) as a control
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- P14136-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.3% Glutathione
Sequence info
Amino acid sequence
- M E R R R I T S A A R R S Y V S S G E M M V G G L A P G R R L G P G T R L S L A R M P P P L P T R V D F S L A G A L N A G F K E T R A S E R A E M M E L N D R F A S Y I E K V R F L E Q Q N K A L A A E L N Q L R A K E P T K L A D V Y Q A E L R E L R L R L D Q L T A N S A R L E V E R D N L A Q D L A T V R Q K L Q D E T N L R L E A E N N L A A Y R Q E A D E A T L A R L D L E R K I E S L E E E I R F L R K I H E E E V R E L Q E Q L A R Q Q V H V E L D V A K P D L T A A L K E I R T Q Y E A M A S S N M H E A E E W Y R S K F A D L T D A A A R N A E L L R Q A K H E A N D Y R R Q L Q S L T C D L E S L R G T N E S L E R Q M R E Q E E R H V R E A A S Y Q E A L A R L E E E G Q S L K D E M A R H L Q E Y Q D L L N V K L A L D I E I A T Y R K L L E G E E N R I T I P V Q T F S N L Q I R E T S L D T K S V S E G H L K R N I V V K T V E M R D G E V I K E S K Q E H K D V M
- Accession
- P14136
- Protein length
- Full Length
- Predicted molecular weight
- 76 kDa
- Amino acids
- 1 to 432
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Glutathione Sepharose 4 Fast Flow
General info
- Function
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
- Sequence similarities
Belongs to the intermediate filament family.
- Post-translational modifications
Phosphorylated by PKN1.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Ensure the validity of your result using our recombinant human GFAP protein ab114149 as a positive control in western blot and SDS-PAGE.
Analyze your GFAP ELISA data using the ab114149 protein to generate and plot a standard curve.
Check out our protein gel staining guide for SDS-PAGE here
Check out of western blot protocol for more information here
Check out our ELISA protocol for more information here.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
SDS-PAGE - Recombinant Human GFAP protein (ab114149)
12.5% SDS-PAGE analysis of ab114149, stained with Coomassie Blue.
Western blot - Recombinant Human GFAP protein (ab114149)
All lanes: Anti-GFAP antibody - Astrocyte Marker (ab48050) at 1 µg/mL
All lanes: Western blot - Recombinant Human GFAP protein (ab114149) at 0.1 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10s
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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