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Recombinant Human GFAP protein is a Human Full Length protein, in the 1 to 432 aa range, expressed in Wheat germ and suitable for SDS-PAGE, western blot and ELISA. The predicted molecular weight of ab114149 protein is 76 kDa.

- Save time and ensure accurate results - use our recombinant Glial fibrillary acidic protein (GFAP) as a control

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Images

SDS-PAGE - Recombinant Human GFAP protein (AB114149), expandable thumbnail
  • Western blot - Recombinant Human GFAP protein (AB114149), expandable thumbnail

Publications

Key facts

Expression system
Wheat germ
Tags
GST tag N-Terminus
Applications
SDS-PAGE, ELISA, WB
Biologically active
No

Amino acid sequence

M E R R R I T S A A R R S Y V S S G E M M V G G L A P G R R L G P G T R L S L A R M P P P L P T R V D F S L A G A L N A G F K E T R A S E R A E M M E L N D R F A S Y I E K V R F L E Q Q N K A L A A E L N Q L R A K E P T K L A D V Y Q A E L R E L R L R L D Q L T A N S A R L E V E R D N L A Q D L A T V R Q K L Q D E T N L R L E A E N N L A A Y R Q E A D E A T L A R L D L E R K I E S L E E E I R F L R K I H E E E V R E L Q E Q L A R Q Q V H V E L D V A K P D L T A A L K E I R T Q Y E A M A S S N M H E A E E W Y R S K F A D L T D A A A R N A E L L R Q A K H E A N D Y R R Q L Q S L T C D L E S L R G T N E S L E R Q M R E Q E E R H V R E A A S Y Q E A L A R L E E E G Q S L K D E M A R H L Q E Y Q D L L N V K L A L D I E I A T Y R K L L E G E E N R I T I P V Q T F S N L Q I R E T S L D T K S V S E G H L K R N I V V K T V E M R D G E V I K E S K Q E H K D V M

Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Application
ELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Application
WB
Reactivity
Reacts
Dilution info
-
Notes

ab114149 can be used as a WB positive control in conjunction with ab48050.

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

Alternative names

Recommended products

Recombinant Human GFAP protein is a Human Full Length protein, in the 1 to 432 aa range, expressed in Wheat germ and suitable for SDS-PAGE, western blot and ELISA. The predicted molecular weight of ab114149 protein is 76 kDa.

- Save time and ensure accurate results - use our recombinant Glial fibrillary acidic protein (GFAP) as a control

Key facts

Expression system
Wheat germ
Applications
SDS-PAGE, ELISA, WB
Accession
P14136-1
Animal free
No
Species
Human
Concentration
Loading...
Storage buffer

pH: 8
Constituents: 0.79% Tris HCl, 0.3% Glutathione

Sequence info

Amino acid sequence

M E R R R I T S A A R R S Y V S S G E M M V G G L A P G R R L G P G T R L S L A R M P P P L P T R V D F S L A G A L N A G F K E T R A S E R A E M M E L N D R F A S Y I E K V R F L E Q Q N K A L A A E L N Q L R A K E P T K L A D V Y Q A E L R E L R L R L D Q L T A N S A R L E V E R D N L A Q D L A T V R Q K L Q D E T N L R L E A E N N L A A Y R Q E A D E A T L A R L D L E R K I E S L E E E I R F L R K I H E E E V R E L Q E Q L A R Q Q V H V E L D V A K P D L T A A L K E I R T Q Y E A M A S S N M H E A E E W Y R S K F A D L T D A A A R N A E L L R Q A K H E A N D Y R R Q L Q S L T C D L E S L R G T N E S L E R Q M R E Q E E R H V R E A A S Y Q E A L A R L E E E G Q S L K D E M A R H L Q E Y Q D L L N V K L A L D I E I A T Y R K L L E G E E N R I T I P V Q T F S N L Q I R E T S L D T K S V S E G H L K R N I V V K T V E M R D G E V I K E S K Q E H K D V M
Accession
P14136
Protein length
Full Length
Predicted molecular weight
76 kDa
Amino acids
1 to 432
Nature
Recombinant
Tags
GST tag N-Terminus

Specifications

Form
Liquid
Additional notes

Glutathione Sepharose 4 Fast Flow

General info

Function

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

Sequence similarities

Belongs to the intermediate filament family.

Post-translational modifications

Phosphorylated by PKN1.

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Ensure the validity of your result using our recombinant human GFAP protein ab114149 as a positive control in western blot and SDS-PAGE.

Analyze your GFAP ELISA data using the ab114149 protein to generate and plot a standard curve.


Check out our protein gel staining guide for SDS-PAGE here

Check out of western blot protocol for more information here

Check out our ELISA protocol for more information here.


Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • SDS-PAGE - Recombinant Human GFAP protein (ab114149), expandable thumbnail

    SDS-PAGE - Recombinant Human GFAP protein (ab114149)

    12.5% SDS-PAGE analysis of ab114149, stained with Coomassie Blue.

  • Western blot - Recombinant Human GFAP protein (ab114149), expandable thumbnail

    Western blot - Recombinant Human GFAP protein (ab114149)

    All lanes: Anti-GFAP antibody - Astrocyte Marker (ab48050) at 1 µg/mL

    All lanes: Western blot - Recombinant Human GFAP protein (ab114149) at 0.1 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 10s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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