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AB114149

Recombinant Human GFAP protein (GST tag N-Terminus)

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(6 Publications)

Recombinant Human GFAP protein is a Human Full Length protein, in the 1 to 432 aa range, expressed in Wheat germ and suitable for SDS-PAGE, western blot and ELISA. The predicted molecular weight of ab114149 protein is 76 kDa.

- Save time and ensure accurate results - use our recombinant Glial fibrillary acidic protein (GFAP) as a control

View Alternative Names

Glial fibrillary acidic protein, GFAP

2 Images
Western blot - Recombinant Human GFAP protein (GST tag N-Terminus) (AB114149)
  • WB

Unknown

Western blot - Recombinant Human GFAP protein (GST tag N-Terminus) (AB114149)

All lanes:

Anti-GFAP antibody - Astrocyte Marker (<a href='/en-us/products/unavailable/gfap-antibody-astrocyte-marker-ab48050'>ab48050</a>) at 1 µg/mL

All lanes:

Western blot - Recombinant Human GFAP protein (GST tag N-Terminus) (ab114149) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

true

Exposure time: 10s

SDS-PAGE - Recombinant Human GFAP protein (GST tag N-Terminus) (AB114149)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human GFAP protein (GST tag N-Terminus) (AB114149)

12.5% SDS-PAGE analysis of ab114149, stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

ELISA, WB, SDS-PAGE

applications

Biologically active

No

Accession

P14136

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB68428

Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Multiplex immunohistochemistry - Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB68428)

  • 5
  • 20
Proteins & Peptides

AB224840

Recombinant Human 68kDa Neurofilament/NF-L protein

Mass Spectrometry - Recombinant Human 68kDa Neurofilament/NF-L protein (AB224840)

  • 5
  • 1
Assay Kits

AB288175

Human GFAP ELISA Kit

Recombinant|SimpleStep

ELISA - Human GFAP ELISA Kit (AB288175)

  • 5
  • 2
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab114149 can be used as a WB positive control in conjunction with <a href='/en-us/products/unavailable/gfap-antibody-astrocyte-marker-ab48050'>ab48050</a>.</p>" } } }
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Product details

Ensure the validity of your result using our recombinant human GFAP protein ab114149 as a positive control in western blot and SDS-PAGE.

Analyze your GFAP ELISA data using the ab114149 protein to generate and plot a standard curve.


Check out our protein gel staining guide for SDS-PAGE here

Check out of western blot protocol for more information here

Check out our ELISA protocol for more information here.
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Sequence info

[{"sequence":"MERRRITSAARRSYVSSGEMMVGGLAPGRRLGPGTRLSLARMPPPLPTRVDFSLAGALNAGFKETRASERAEMMELNDRFASYIEKVRFLEQQNKALAAELNQLRAKEPTKLADVYQAELRELRLRLDQLTANSARLEVERDNLAQDLATVRQKLQDETNLRLEAENNLAAYRQEADEATLARLDLERKIESLEEEIRFLRKIHEEEVRELQEQLARQQVHVELDVAKPDLTAALKEIRTQYEAMASSNMHEAEEWYRSKFADLTDAAARNAELLRQAKHEANDYRRQLQSLTCDLESLRGTNESLERQMREQEERHVREAASYQEALARLEEEGQSLKDEMARHLQEYQDLLNVKLALDIEIATYRKLLEGEENRITIPVQTFSNLQIRETSLDTKSVSEGHLKRNIVVKTVEMRDGEVIKESKQEHKDVM","proteinLength":"Full Length","predictedMolecularWeight":"76 kDa","actualMolecularWeight":null,"aminoAcidEnd":432,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P14136","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

Additional notes

Glutathione Sepharose 4 Fast Flow

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General info

Function

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

Sequence similarities

Belongs to the intermediate filament family.

Post-translational modifications

Phosphorylated by PKN1.

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Product protocols

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Target data

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
See full target information GFAP
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Medicine 102:e32885 PubMed36820591

2023

The neuroprotective effect of electro-acupuncture on cognitive recovery for patients with mild traumatic brain injury: A randomized controlled clinical trial.

Applications

Unspecified application

Species

Unspecified reactive species

Haokun Jia,Yonghan Chen,Yi Wang,Linwei Jia,Yaohui Tian,Hao Jiang

Lupus science & medicine 9: PubMed35705307

2022

Role of autoantibodies and blood-brain barrier leakage in cognitive impairment in systemic lupus erythematosus.

Applications

Unspecified application

Species

Unspecified reactive species

John G Hanly,Alexandra Legge,Lyna Kamintsky,Alon Friedman,Javeria A Hashmi,Steven D Beyea,John Fisk,Antonina Omisade,Cynthia Calkin,Tim Bardouille,Chris Bowen,Kara Matheson,Marvin J Fritzler

Journal of neural transmission (Vienna, Austria : 1996) 129:545-555 PubMed35364741

2022

Plasma autoantibodies to glial fibrillary acidic protein (GFAP) react with brain areas according to Braak staging of Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Eva Gschmack,Camelia-Maria Monoranu,Hecham Marouf,Sarah Meyer,Lena Lessel,Raja Idris,Daniela Berg,Walter Maetzler,Frank Steigerwald,Jens Volkmann,Manfred Gerlach,Peter Riederer,Eleni Koutsilieri,Carsten Scheller

Nanoscale advances 4:1770-1778 PubMed35434521

2022

Carbon dot-based fluorescent antibody nanoprobes as brain tumour glioblastoma diagnostics.

Applications

Unspecified application

Species

Unspecified reactive species

Mattia Ghirardello,Radhe Shyam,Xia Liu,Teodoro Garcia-Millan,Imke Sittel,Javier Ramos-Soriano,Kathreena M Kurian,M Carmen Galan

Neuropsychiatric disease and treatment 16:2633-2647 PubMed33177827

2020

Transcription Factor E2F1 Aggravates Neurological Injury in Ischemic Stroke via microRNA-122-Targeted Sprouty2.

Applications

Unspecified application

Species

Unspecified reactive species

Yunxia Wu,Zhiqiang Gao,Jiang Zhang

Biosensors & bioelectronics 75:420-6 PubMed26356762

2015

Microwave-accelerated bioassay technique for rapid and quantitative detection of biological and environmental samples.

Applications

Unspecified application

Species

Unspecified reactive species

Muzaffer Mohammed,Maleeha F Syed,Kadir Aslan
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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