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AB89233

Recombinant Human Ghrelin protein (His tag N-Terminus)

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(1 Publication)

Recombinant Human Ghrelin protein (His tag N-Terminus) is a Human Full Length protein, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, WB.

View Alternative Names

MTLRP, UNQ524/PRO1066, GHRL, Appetite-regulating hormone, Growth hormone secretagogue, Growth hormone-releasing peptide, Motilin-related peptide, Protein M46

2 Images
Western blot - Recombinant Human Ghrelin protein (His tag N-Terminus) (AB89233)
  • WB

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Western blot - Recombinant Human Ghrelin protein (His tag N-Terminus) (AB89233)

All lanes:

Anti-Ghrelin antibody (<a href='/en-us/products/unavailable/ghrelin-antibody-ab57222'>ab57222</a>) at 1 µg/mL

All lanes:

Western blot - Recombinant Human Ghrelin protein (His tag N-Terminus) (ab89233) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

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Exposure time: 2min

SDS-PAGE - Recombinant Human Ghrelin protein (AB89233)
  • SDS-PAGE

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SDS-PAGE - Recombinant Human Ghrelin protein (AB89233)

15% SDS-PAGE showing ab89233 (3μg).
Note : Real molecular weight on SDS-PAGE is shifted up compared to predicted molecular weight.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

WB, SDS-PAGE

applications

Biologically active

No

Accession

Q9UBU3

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 1.16% Sodium chloride, 0.32% Tris HCl, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab89233 can be used as a WB positive control in conjunction with <a href='/en-us/products/unavailable/ghrelin-antibody-ab57222'>ab57222</a>.</p>" } } }
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Sequence info

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q9UBU3","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ghrelin also known as the "hunger hormone" or GH secretagogue is a peptide hormone with a mass of 28 amino acids. Ghrelin is mainly expressed in the stomach particularly in the enteroendocrine cells but it can also be found in small amounts in the lungs pancreas and hypothalamus. The hormone increases food intake and promotes fat storage by signaling the brain to stimulate hunger. Its secretion occurs in response to fasting and is suppressed by food intake reflecting its role in energy balance.
Biological function summary

Ghrelin influences a variety of physiological processes beyond hunger regulation. It plays a role in modulating the release of growth hormone by acting on the growth hormone secretagogue receptor (GHS-R). Ghrelin also affects gastric motility and acid secretion due to its expression in the gastrointestinal tract. Though not part of a larger complex its interaction with GHS-R strongly influences its biological functions. Ghrelin may also impact insulin secretion and sensitivity linking it to glucose metabolism processes.

Pathways

Ghrelin interacts with several critical biological networks. Ghrelin is involved in the neuroendocrine pathway that connects the gut to the brain and contributes significantly to energy homeostasis. Within this pathway it influences proteins like leptin which exerts opposite effects by suppressing hunger. Additionally ghrelin functions in the hedonic control of eating which relates to the rewarding aspects of food intake controlled by pathways involving dopamine.

Ghrelin holds relevance to obesity and cachexia a form of extreme weight loss often seen in chronic illness. Ghrelin promotes adiposity by stimulating appetite which can contribute to obesity. Conversely in cachexia the decrease in ghrelin activity is thought to exacerbate weight loss. Its role in energy balance associates ghrelin with the protein leptin which counterbalances its effects and connections to metabolic disorders. Understanding ghrelin's functions and pathways provides insight into therapeutic strategies for these conditions.
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Specifications

Form

Liquid

Additional notes

ab89233 is purified using conventional chromatography techniques.Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method).

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General info

Function

Ghrelin-27. Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR) (PubMed : 10604470). Induces the release of growth hormone from the pituitary (PubMed : 10604470). Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.. Ghrelin-28. Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR) (PubMed : 10604470). Induces the release of growth hormone from the pituitary (PubMed : 10604470). Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.. Obestatin. May be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility (By similarity).

Sequence similarities

Belongs to the motilin family.

Post-translational modifications

O-octanoylated by GOAT/MBOAT4 (PubMed:18443287). O-octanoylation or O-decanoylation is essential for ghrelin activity (PubMed:10604470). The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28-C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26 (PubMed:10604470). A small fraction of ghrelin, ghrelin-28-C10:1, may be modified with a singly unsaturated carboxylic acid (PubMed:10604470). Also O-acetylated and O-butyrylated on Ser-26 to minor levels (PubMed:18443287).. Amidation of Leu-98 is essential for obestatin activity.

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Product protocols

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Target data

Ghrelin-27. Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR) (PubMed : 10604470). Induces the release of growth hormone from the pituitary (PubMed : 10604470). Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.. Ghrelin-28. Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR) (PubMed : 10604470). Induces the release of growth hormone from the pituitary (PubMed : 10604470). Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.. Obestatin. May be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility (By similarity).
See full target information GHRL
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of biomolecular screening 18:1035-42 PubMed23704134

2013

Establishment of a gastric cell-based assay system for exploring inhibitors of octanoylated ghrelin production.

Applications

Unspecified application

Species

Unspecified reactive species

Shigeru Oiso,Miyuki Nobe,Yuhei Yamaguchi,Shigeru Umemoto,Kazuo Nakamura,Hiroko Kariyazono
View all publications
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