Recombinant Human GILT protein (His tag)
Be the first to review this product! Submit a review
|
(0 Publication)
Recombinant Human GILT protein (His tag) is a Human Fragment protein, in the 1 to 243 aa range, expressed in HEK 293 cells, with >96%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.
View Alternative Names
GILT, IP30, IFI30, Gamma-interferon-inducible lysosomal thiol reductase, Gamma-interferon-inducible protein IP-30, Legumaturain
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Human GILT protein (His tag) (AB276486)
SDS-PAGE analysis of ab276486
Reactivity data
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
GILT contributes to the processing and presentation of antigens. By reducing disulfide bonds in endocytosed proteins GILT prepares them for subsequent degradation by lysosomal proteases. This function means it plays a significant role in the immune response as it aids the generation of peptides presented on major histocompatibility complex (MHC) class II molecules. GILT is not known to be part of a larger protein complex but interacts with other components within the lysosome to accomplish its task.
Pathways
GILT is integral to the antigen processing and presentation pathway mediated by MHC class II molecules. This pathway is critical for the initiation of immune responses and involves collaboration with other proteins such as cathepsins. The reduction of disulfide bonds by GILT allows cathepsins to efficiently degrade proteins into peptide fragments. These peptides then bind to MHC class II molecules which are presented on the cell surface for recognition by CD4+ T cells.
Specifications
Form
Lyophilized
General info
Function
Lysosomal thiol reductase that can reduce protein disulfide bonds. May facilitate the complete unfolding of proteins destined for lysosomal degradation. Plays an important role in antigen processing. Facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds (By similarity). Facilitates also MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or crosspresentation (By similarity).
Sequence similarities
Belongs to the GILT family.
Post-translational modifications
N-glycosylated. Sugar chains contain mannose-6-phosphate.. Synthesized as a 35 kDa precursor which is then processed into the mature 30 kDa form via cleavage of N-terminal and C-terminal propeptides. Processing of the precursor is mediated by multiple lysosomal proteases.
Subcellular localisation
Lysosome
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com