Recombinant Human GIP protein is a Human Full Length protein, in the 22 to 153 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S E K K E G H F S A L P S L P V G S H A K V S S P Q P R G P R Y A E G T F I S D Y S I A M D K I H Q Q D F V N W L L A Q K G K K N D W K H N I T Q R E A R A L E L A G Q A N R K E E E A V E P Q S S P A K N P S D E D L L R D L L I Q E L L A C L L D Q T N L C R L R S R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Potent stimulator of insulin secretion and relatively poor inhibitor of gastric acid secretion.
Alternative names
Gastric inhibitory polypeptide, GIP, Glucose-dependent insulinotropic polypeptide, Incretin hormone
Recommended products
Recombinant Human GIP protein is a Human Full Length protein, in the 22 to 153 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P09681-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 50% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S E K K E G H F S A L P S L P V G S H A K V S S P Q P R G P R Y A E G T F I S D Y S I A M D K I H Q Q D F V N W L L A Q K G K K N D W K H N I T Q R E A R A L E L A G Q A N R K E E E A V E P Q S S P A K N P S D E D L L R D L L I Q E L L A C L L D Q T N L C R L R S R
- Accession
- P09681
- Protein length
- Full Length
- Predicted molecular weight
- 17.3 kDa
- Amino acids
- 22 to 153
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab156354 is purified using anion exchange chromatography (DEAE sepharose resin) and gel filtration chromatography (Sephacryl S-200) with 20 mM Tris pH 7.5, 2 mM EDTA.
General info
- Function
Potent stimulator of insulin secretion and relatively poor inhibitor of gastric acid secretion.
- Sequence similarities
Belongs to the glucagon family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Glucose-dependent Insulinotropic Polypeptide (GIP) also known as gastric inhibitory polypeptide is a hormone with a molecular mass around 5 kDa. It is a 42-amino acid peptide secreted by the K cells in the duodenum and jejunum of the small intestine. This protein interacts with the GIP receptor which is found in various tissues including pancreatic beta cells adipose tissue and the central nervous system. Researchers utilize GIP ELISA for detecting and quantifying this peptide in research studies.
- Biological function summary
The GIP plays a significant role in stimulating insulin secretion in response to glucose intake which does not function alone. It works as part of the enteroinsular axis influencing nutrient uptake and metabolism. GIP also regulates fat metabolism by acting on adipocytes therefore playing a part in energy homeostasis. The exploration of GIP activity often involves MSD assay development to better understand its biological implications.
- Pathways
The glucose-stimulated insulin secretion pathway incorporates GIP to enhance the release of insulin from the pancreas. It interacts with other proteins such as insulin and glucagon-like peptide-1 (GLP-1) to exert its glucose-lowering effects. GIP's involvement in the incretin pathway supports the body's maintenance of energy balance and glucose homeostasis under normal physiological conditions.
- Associated diseases and disorders
Glucose homeostasis dysfunction links GIP to type 2 diabetes and obesity. Impairments in GIP secretion or receptor signaling are associated with these metabolic disorders. Studies suggest that dysregulation of GIP along with related proteins like insulin can contribute to disease progression. By targeting GIP researchers at places like Abcam are looking for potential therapies to tackle these health challenges.
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1 product image
SDS-PAGE - Recombinant Human GIP protein (ab156354)
15% SDS-PAGE analysis of ab156354 (3 μg).
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