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AB151890

Recombinant Human GLB1/Beta-galactosidase protein

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Recombinant Human GLB1/Beta-galactosidase protein is a Human Full Length protein, in the 24 to 677 aa range, expressed in HEK 293 cells, with >95%, < 0.1 EU/µg endotoxin level, suitable for SDS-PAGE, HPLC.

View Alternative Names

ELNR1, GLB1, Beta-galactosidase, Acid beta-galactosidase, Elastin receptor 1, Lactase

1 Images
Western blot - Recombinant Human GLB1/Beta-galactosidase protein (AB151890)
  • WB

Lab

Western blot - Recombinant Human GLB1/Beta-galactosidase protein (AB151890)

All lanes:

Western blot - Anti-beta Galactosidase antibody [DC1 4C7] (<a href='/en-us/products/primary-antibodies/beta-galactosidase-antibody-dc1-4c7-ab116'>ab116</a>) at 1 µg

Lane 1:

Western blot - Recombinant Human GLB1/Beta-galactosidase protein (ab151890) at 0.1 µg

Lane 2:

Western blot - Native E. coli beta Galactosidase protein (<a href='/en-us/products/proteins-peptides/native-e-coli-beta-galactosidase-protein-ab79449'>ab79449</a>) at 0.1 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/5000 dilution

Predicted band size: 116 kDa,118 kDa,183 kDa,43 kDa,54 kDa,56 kDa,72 kDa,74 kDa,84 kDa

false

Exposure time: 4min

Key facts

Purity

>95% SDS-PAGE

Endotoxin level

< 0.1 EU/µg

Expression system

HEK 293 cells

Tags

His tag C-Terminus

Applications

HPLC, SDS-PAGE

applications

Biologically active

No

Accession

P16278

Animal free

No

Carrier free

Yes

Species

Human

Storage buffer

pH: 8 Constituents: 0.88% Sodium chloride, 0.32% Tris HCl

storage-buffer

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Complementary Products

Primary Antibodies

AB203749

Anti-GLB1/Beta-galactosidase antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLB1/Beta-galactosidase antibody (AB203749)

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  • 0
Proteins & Peptides

AB56278

Recombinant Human p21 protein

Sandwich ELISA - Recombinant Human p21 protein (AB56278)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB116

Anti-beta Galactosidase antibody [DC1 4C7]

Trial Size

Western blot - Anti-beta Galactosidase antibody [DC1 4C7] (AB116)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"NATQRMFEIDYSRDSFLKDGQPFRYISGSIHYSRVPRFYWKDRLLKMKMAGLNAIQTYVPWNFHEPWPGQYQFSEDHDVEYFLRLAHELGLLVILRPGPYICAEWEMGGLPAWLLEKESILLRSSDPDYLAAVDKWLGVLLPKMKPLLYQNGGPVITVQVENEYGSYFACDFDYLRFLQKRFRHHLGDDVVLFTTDGAHKTFLKCGALQGLYTTVDFGTGSNITDAFLSQRKCEPKGPLINSEFYTGWLDHWGQPHSTIKTEAVASSLYDILARGASVNLYMFIGGTNFAYWNGANSPYAAQPTSYDYDAPLSEAGDLTEKYFALRNIIQKFEKVPEGPIPPSTPKFAYGKVTLEKLKTVGAALDILCPSGPIKSLYPLTFIQVKQHYGFVLYRTTLPQDCSNPAPLSSPLNGVHDRAYVAVDGIPQGVLERNNVITLNITGKAGATLDLLVENMGRVNYGAYINDFKGLVSNLTLSSNILTDWTIFPLDTEDAVRSHLGGWGHRDSGHHDEAWAHNSSNYTLPAFYMGNFSIPSGIPDLPQDTFIQFPGWTKGQVWINGFNLGRYWPARGPQLTLFVPQHILMTSAPNTITVLELEWAPCSSDDPELCAVTFVDRPVIGSSVTYDHPSKPVEKRLMPPPPQKNKDSWLDHVVDHHHHHH","proteinLength":"Full Length","predictedMolecularWeight":"74.37 kDa","actualMolecularWeight":null,"aminoAcidEnd":677,"aminoAcidStart":24,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P16278","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GLB1 also known as beta-galactosidase is an enzyme that catalyzes the hydrolysis of beta-galactosides into monosaccharides. The protein has a molecular mass of approximately 76 kDa. Researchers observe this enzyme expression in the lysosome where it functions to break down complex carbohydrates. Its activity depends on proper glycosylation and correct folding usually occurring within specific cellular compartments.
Biological function summary

GLB1 is integral to the degradation and recycling of glycoproteins and glycolipids in the cell. It forms part of the lysosomal enzyme complex responsible for processing macromolecules. Without its function the accumulation of substrates can occur affecting cellular metabolism. The enzyme ensures that metabolic waste is efficiently processed linking GLB1 to cellular homeostasis.

Pathways

Beta-galactosidase participates in the lysosomal storage and glycan metabolism pathways. It is important for the catabolism of glycoside forms of hormones and other biologically-active molecules. In these pathways GLB1 interacts with proteins such as hexosaminidase creating a network of lysosomal enzymes. These interactions enable coordinated degradation of glycoproteins maintaining balance within cellular systems.

GLB1 mutations associate with conditions like GM1 gangliosidosis and Morquio syndrome type B. These are lysosomal storage disorders resulting from the accumulation of unmetabolized substrates. In these diseases hexosaminidase and other lysosomal enzymes show relationships with GLB1 illuminating complex interactions within cellular pathology. Therapeutic research aims to correct or enhance enzyme function seeking to alleviate disorder symptoms.
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Specifications

Form

Liquid

Additional notes

ab151890 has greater than 95% purity as determined by SEC-HPLC and reducing SDS-PAGE.

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General info

Function

Isoform 1. Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.. Isoform 2. Has no beta-galactosidase catalytic activity, but plays functional roles in the formation of extracellular elastic fibers (elastogenesis) and in the development of connective tissue. Seems to be identical to the elastin-binding protein (EBP), a major component of the non-integrin cell surface receptor expressed on fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. In elastin producing cells, associates with tropoelastin intracellularly and functions as a recycling molecular chaperone which facilitates the secretions of tropoelastin and its assembly into elastic fibers.

Sequence similarities

Belongs to the glycosyl hydrolase 35 family.

Subcellular localisation

Lysosome

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Product protocols

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Target data

Isoform 1. Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.. Isoform 2. Has no beta-galactosidase catalytic activity, but plays functional roles in the formation of extracellular elastic fibers (elastogenesis) and in the development of connective tissue. Seems to be identical to the elastin-binding protein (EBP), a major component of the non-integrin cell surface receptor expressed on fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. In elastin producing cells, associates with tropoelastin intracellularly and functions as a recycling molecular chaperone which facilitates the secretions of tropoelastin and its assembly into elastic fibers.
See full target information GLB1
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