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AB134895

Recombinant human GLIS1 protein

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Recombinant human GLIS1 protein is a Human Full Length protein, in the 2 to 620 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, FuncS.

View Alternative Names

Zinc finger protein GLIS1, GLI-similar 1, GLIS1

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Active

Accession

Q8NBF1

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 3.4% DL-Arginine, 0.32% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% EDTA

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"2-8 µg/mL", "notes":"<p>For studying Human direct reprogramming of somatic cells.</p>" } } }
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Product details

Protein transduction for in vitro somatic cell reprogramming study.
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Sequence info

[{"sequence":"AEARTSLSAHCRGPLATGLHPDLDLPGRSLATPAPSCYLLGSEPSSGLGLQPETHLPEGSLKRCCVLGLPPTSPASSSPCASSDVTSIIRSSQTSLVTCVNGLRSPPLTGDLGGPSKRARPGPASTDSHEGSLQLEACRKASFLKQEPADEFSELFGPHQQGLPPPYPLSQLPPGPSLGGLGLGLAGRVVAGRQACRWVDCCAAYEQQEELVRHIEKSHIDQRKGEDFTCFWAGCVRRYKPFNARYKLLIHMRVHSGEKPNKCMFEGCSKAFSRLENLKIHLRSHTGEKPYLCQHPGCQKAFSNSSDRAKHQRTHLDTKPYACQIPGCSKRYTDPSSLRKHVKAHSAKEQQVRKKLHAGPDTEADVLTECLVLQQLHTSTQLAASDGKGGCGLGQELLPGVYPGSITPHNGLASGLLPPAHDVPSRHHPLDATTSSHHHLSPLPMAESTRDGLGPGLLSPIVSPLKGLGPPPLPPSSQSHSPGGQPFPTLPSKPSYPPFQSPPPPPLPSPQGYQGSFHSIQSCFPYGDCYRMAEPAAGGDGLVGETHGFNPLRPNGYHSLSTPLPATGYEALAEASCPTALPQQPSEDVVSSGPEDCGFFPNGAFDHCLGHIPSIYTDTLEESGGGGSPGRRRRRRRRRRR","proteinLength":"Full Length","predictedMolecularWeight":"68.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":620,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q8NBF1","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GLIS1 also known as GLI-Similar 1 is a Krüppel-like zinc finger protein with a mass of approximately 91 kDa. It acts as a transcription factor that regulates gene expression by binding to specific DNA sequences. GLIS1 is expressed mainly in pluripotent stem cells and certain adult tissues including the brain and kidney. It holds a significant role in modulating transcriptional programs important for cellular differentiation and development.
Biological function summary

GLIS1 influences various developmental processes and cellular functions. It does not appear as a part of a larger complex but instead functions independently to regulate genes involved in maintaining and establishing cellular identity. Through its activity it directly impacts cell fate decisions during early development making it important for stem cell biology. GLIS1 can activate genes that are essential for pluripotency and stimulate the reprogramming of somatic cells back into induced pluripotent stem cells (iPSCs).

Pathways

GLIS1 plays key roles in both the Hedgehog and Wnt signaling pathways. It can regulate the expression of components within these pathways impacting cellular growth and differentiation processes. In the context of the Hedgehog pathway GLIS1 interacts with proteins such as GLI1 and GLI2 while in the Wnt pathway it influences the behavior of proteins like β-catenin. These interactions help to fine-tune the transcriptional outcomes important for developmental signaling.

GLIS1 has implications in cancers and neurodegenerative diseases. Abnormal expression or malfunction of GLIS1 can lead to disruptions in normal cell growth and differentiation contributing to oncogenesis. It may connect with proteins like p53 in tumor suppression. Additionally its role in the central nervous system suggests links to neurodegenerative conditions where maintaining neural stem cell populations is critical. Abnormal GLIS1 function can therefore contribute to disease progression in these contexts.
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Specifications

Form

Liquid

Additional notes

ab134895 was expressed in E. coli as inclusion bodies, refolded using temperature shift inclusion body refolding technology, chromatographically purified and sterile-filtered.

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General info

Function

Acts both as a repressor and an activator of transcription (PubMed : 21654807). Binds to the consensus sequence 5'-GACCACCCAC-3' (By similarity). By controlling the expression of genes involved in cell differentiation inhibits the lineage commitment of multipotent cells (PubMed : 21654807). Prevents, for instance, the differentiation of multipotent mesenchymal cells into adipocyte and osteoblast (By similarity).

Sequence similarities

Belongs to the GLI C2H2-type zinc-finger protein family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Acts both as a repressor and an activator of transcription (PubMed : 21654807). Binds to the consensus sequence 5'-GACCACCCAC-3' (By similarity). By controlling the expression of genes involved in cell differentiation inhibits the lineage commitment of multipotent cells (PubMed : 21654807). Prevents, for instance, the differentiation of multipotent mesenchymal cells into adipocyte and osteoblast (By similarity).
See full target information Zinc finger protein GLIS1
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Product promise

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