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AB102019

Recombinant Human GLO2 protein

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Recombinant Human GLO2 protein is a Human Full Length protein, in the 1 to 260 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

GLO2, HAGH1, HAGH, Glyoxalase II, Glx II

1 Images
SDS-PAGE - Recombinant Human GLO2 protein (AB102019)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human GLO2 protein (AB102019)

15% SDS-PAGE analysis of 3 μg ab102019.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q16775

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8.5 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as HAGH
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMKVEVLPALTDNYMYLVIDDETKEAAIVDPVQPQKVVDAARKHGVKLTTVLTTHHHWDHAGGNEKLVKLESGLKVYGGDDRIGALTHKITHLSTLQVGSLNVKCLATPCHTSGHICYFVSKPGGSEPPAVFTGDTLFVAGCGKFYEGTADEMCKALLEVLGRLPPDTRVYCGHEYTINNLKFARHVEPGNAAIREKLAWAKEKYSIGEPTVPSTLAEEFTYNPFMRVREKTVQQHAGETDPVTTMRAVRREKDQFKMPRD","proteinLength":"Full Length","predictedMolecularWeight":"31.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":260,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q16775","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glyoxalase 2 also known as GLO2 is an important enzyme in the glyoxalase system. The protein's molecular mass is approximately 29 kDa. It catalyzes the conversion of S-D-lactoylglutathione to glutathione and D-lactic acid allowing the detoxification of methylglyoxal a byproduct of glycolysis. GLO2 is expressed in many tissues with higher levels in liver and kidney reflecting its importance in detoxifying processes.
Biological function summary

Glyoxalase 2 has a significant role in cellular detoxification and maintenance of redox balance. As part of the glyoxalase system it closely works with another enzyme glyoxalase 1 to detoxify methylglyoxal. Through this interaction GLO2 helps prevent harmful effects caused by excessive levels of advanced glycation end-products (AGEs) protecting cells from potential damage.

Pathways

Glyoxalase 2 is involved in the methylglyoxal degradation pathway within the cellular glucose metabolism process. Specifically it partners with glyoxalase 1 in this detoxification route. Additionally GLO2 contributes to glutathione metabolism where it plays a critical part alongside glutathione peroxidases which further regulate oxidative stress.

GLO2 is associated with diabetes and neurodegenerative diseases. Altered levels of glyoxalase 2 impact methylglyoxal levels and have implications in diabetic complications through protein glycation. Furthermore studies indicate its potential link with Alzheimer's disease where oxidative stress and AGEs accumulation play central roles. GLO2's connection with glyoxalase 1 remains significant as both participate in these pathological states.
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Specifications

Form

Liquid

Additional notes

ab102019 was purified using conventional chromatography.

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General info

Function

Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.

Sequence similarities

Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.

Subcellular localisation

Mitochondrion matrix

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Product protocols

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Target data

Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.
See full target information HAGH
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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

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