Recombinant Human GLP-1R protein (His tag) is a Human Fragment protein, in the 24 to 145 aa range, expressed in Mammalian, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Mammalian
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
R P Q G A T V S L W E T V Q K W R E Y R R Q C Q R S L T E D P P P A T D L F C N R T F D E Y A C W P D G E P G S F V N V S C P W Y L P W A S S V P Q G H V Y R F C T A E G L W L Q K D N S S L P W R D L S E C E E S K R G E R S S P E E Q L L F L Y
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
G-protein coupled receptor for glucagon-like peptide 1 (GLP-1) (PubMed:19861722, PubMed:26308095, PubMed:27196125, PubMed:28514449, PubMed:7517895, PubMed:8216285, PubMed:8405712). Ligand binding triggers activation of a signaling cascade that leads to the activation of adenylyl cyclase and increased intracellular cAMP levels (PubMed:19861722, PubMed:26308095, PubMed:27196125, PubMed:28514449, PubMed:7517895, PubMed:8216285, PubMed:8405712). Plays a role in regulating insulin secretion in response to GLP-1 (By similarity).
Alternative names
Glucagon-like peptide 1 receptor, GLP-1 receptor, GLP-1-R, GLP-1R, GLP1R
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Recombinant Human GLP-1R protein (His tag) is a Human Fragment protein, in the 24 to 145 aa range, expressed in Mammalian, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Mammalian
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P43220-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS, 6% Trehalose
Sequence info
Amino acid sequence
- R P Q G A T V S L W E T V Q K W R E Y R R Q C Q R S L T E D P P P A T D L F C N R T F D E Y A C W P D G E P G S F V N V S C P W Y L P W A S S V P Q G H V Y R F C T A E G L W L Q K D N S S L P W R D L S E C E E S K R G E R S S P E E Q L L F L Y
- Accession
- P43220
- Protein length
- Fragment
- Predicted molecular weight
- 18.3 kDa
- Amino acids
- 24 to 145
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
nan
General info
- Function
G-protein coupled receptor for glucagon-like peptide 1 (GLP-1) (PubMed:19861722, PubMed:26308095, PubMed:27196125, PubMed:28514449, PubMed:7517895, PubMed:8216285, PubMed:8405712). Ligand binding triggers activation of a signaling cascade that leads to the activation of adenylyl cyclase and increased intracellular cAMP levels (PubMed:19861722, PubMed:26308095, PubMed:27196125, PubMed:28514449, PubMed:7517895, PubMed:8216285, PubMed:8405712). Plays a role in regulating insulin secretion in response to GLP-1 (By similarity).
- Sequence similarities
Belongs to the G-protein coupled receptor 2 family.
- Post-translational modifications
N-glycosylation enhances cell surface expression and lengthens receptor half-life by preventing degradation in the ER.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GLP-1R also known as glucagon-like peptide-1 receptor plays an important role in glucose metabolism. This receptor is a member of the class B family of G protein-coupled receptors (GPCRs) and has a molecular mass of approximately 53 kDa. GLP-1R is expressed in pancreatic beta cells the central nervous system and other peripheral tissues. It binds to GLP-1 a peptide hormone initiating a cascade of intracellular signaling that is essential for its physiological functions.
- Biological function summary
GLP-1R serves as an important player in the regulation of insulin secretion. It is not part of a complex but acts as a standalone receptor. When GLP-1 binds to GLP-1R it triggers the activation of adenylyl cyclase increasing cyclic AMP levels and facilitating insulin secretion. This process is glucose-dependent meaning that it enhances insulin release only when blood glucose levels are elevated therefore preventing hypoglycemia.
- Pathways
GLP-1R is an integral component of the incretin signaling pathway and the insulin signaling pathway. Through these pathways it influences key metabolic processes. Incretin hormones like GLP-1 improve glucose-dependent insulin secretion involving proteins such as GLP-1R and other incretin-related receptors. Additionally GLP-1R interacts with insulin receptor pathways to modulate glucose uptake in tissues.
- Associated diseases and disorders
GLP-1R has significant implications in type 2 diabetes and obesity. Its role in enhancing insulin secretion makes it a target for therapeutic interventions using GLP-1 analogs like liraglutide. Liraglutide structure mimics natural GLP-1 effectively agonizing GLP-1R to achieve desired metabolic outcomes. Additionally liraglutide GLP-1 analog therapies show promise in obesity management by influencing appetite regulation through GLP-1R pathways in the central nervous system.
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SDS-PAGE - Recombinant Human GLP-1R protein (His tag) (ab267911)
SDS-PAGE analysis of ab267911 under reducing conditions.
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