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AB126671

Recombinant human Glucose 6 Phosphate Dehydrogenase protein (His tag N-Terminus)

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(1 Publication)

Recombinant human Glucose 6 Phosphate Dehydrogenase protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 515 aa range, expressed in Baculovirus infected BTI-TN-5B1-4 cells, with >95%, suitable for SDS-PAGE, FuncS.

View Alternative Names

Glucose-6-phosphate 1-dehydrogenase, G6PD

1 Images
SDS-PAGE - Recombinant human Glucose 6 Phosphate Dehydrogenase protein (His tag N-Terminus) (AB126671)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human Glucose 6 Phosphate Dehydrogenase protein (His tag N-Terminus) (AB126671)

15% SDS-PAGE analysis of ab126671 (3μg)

Key facts

Purity

>95% SDS-PAGE

Expression system

Baculovirus infected BTI-TN-5B1-4 cells

Tags

His tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Specific activity is > 7 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25°C

Accession

P11413

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.06% EDTA, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMAEQVALSRTQVCGILREELFQGDAFHQSDTHIFIIMGASGDLAKKKIYPTIWWLFRDGLLPENTFIVGYARSRLTVADIRKQSEPFFKATPEEKLKLEDFFARNSYVAGQYDDAASYQRLNSHMNALHLGSQANRLFYLALPPTVYEAVTKNIHESCMSQIGWNRIIVEKPFGRDLQSSDRLSNHISSLFREDQIYRIDHYLGKEMVQNLMVLRFANRIFGPIWNRDNIACVILTFKEPFGTEGRGGYFDEFGIIRDVMQNHLLQMLCLVAMEKPASTNSDDVRDEKVKVLKCISEVQANNVVLGQYVGNPDGEGEATKGYLDDPTVPRGSTTATFAAVVLYVENERWDGVPFILRCGKALNERKAEVRLQFHDVAGDIFHQQCKRNELVIRVQPNEAVYTKMMTKKPGMFFNPEESELDLTYGNRYKNVKLPDAYERLILDVFCGSQMHFVRSDELREAWRIFTPLLHQIELEKPKPIPYIYGSRGPTEADELMKRVGFQYEGTYKWVNPHKL","proteinLength":"Full Length","predictedMolecularWeight":"61.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":515,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected BTI-TN-5B1-4 cells","accessionNumber":"P11413","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glucose 6 Phosphate Dehydrogenase also known as G6PD or G6P dehydrogenase plays an important role in the pentose phosphate pathway catalyzing the conversion of glucose 6-phosphate to 6-phosphoglucono-δ-lactone while producing NADPH from NADP+. G6PD with a molecular mass of approximately 59 kDa is expressed in various tissues with high levels found in red blood cells liver and adrenal glands. This enzyme is vital for protecting cells from oxidative damage by supplying reductive capacity.
Biological function summary

Glucose 6-phosphate dehydrogenase is essential for maintaining cellular redox balance especially in cells lacking mitochondria like red blood cells. It functions as part of a monomer which can dimerize depending on the cellular needs and conditions. The activity of G6PD directly affects the production of NADPH which is necessary for the biosynthesis of nucleic acids and lipids and for maintaining reduced glutathione levels therefore sustaining the antioxidant capacity of the cell.

Pathways

Glucose 6-phosphate dehydrogenase is a pivotal enzyme in the oxidative phase of the pentose phosphate pathway which provides ribose 5-phosphate for nucleotide synthesis and NADPH for reductive biosynthetic reactions. G6PD connects with other enzymes in the pathway such as 6-phosphogluconate dehydrogenase contributing to the regulation of cellular metabolic needs. Its function is tightly linked to glucose metabolism and indirectly influences glycolytic processes.

Alterations in glucose 6-phosphate dehydrogenase activity lead to conditions such as G6PD deficiency which can cause hemolytic anemia when individuals are exposed to certain drugs infections or foods. This enzyme's deficiency is one of the most common enzymatic disorders in humans and is triggered by genetic mutations affecting G6PD function. Moreover G6PD has implications in cancer biology as its activity influences the proliferation and survival of cancer cells alongside other proteins like TP53 and NF-κB which are involved in cellular stress responses.
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Specifications

Form

Liquid

Additional notes

Affinity purified

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General info

Function

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.

Sequence similarities

Belongs to the glucose-6-phosphate dehydrogenase family.

Post-translational modifications

Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity.

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Product protocols

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Target data

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
See full target information Glucose-6-phosphate 1-dehydrogenase
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Science signaling 14:eabe3800 PubMed34784249

2021

Dual NADPH oxidases DUOX1 and DUOX2 synthesize NAADP and are necessary for Ca signaling during T cell activation.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Gu,Aileen Krüger,Hannes G Roggenkamp,Rick Alpers,Dmitri Lodygin,Vincent Jaquet,Franziska Möckl,Lola C Hernandez C,Kai Winterberg,Andreas Bauche,Anette Rosche,Helmut Grasberger,John Y Kao,Daniel Schetelig,René Werner,Katrin Schröder,Michael Carty,Andrew G Bowie,Samuel Huber,Chris Meier,Hans-Willi Mittrücker,Joerg Heeren,Karl-Heinz Krause,Alexander Flügel,Björn-Philipp Diercks,Andreas H Guse
View all publications
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Primary Antibodies

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Assay Kits

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Secondary Antibodies

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