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AB180318

Recombinant Human GLUD1 protein (denatured) (His tag N-Terminus)

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Recombinant Human GLUD1 protein (denatured) (His tag N-Terminus) is a Human Full Length protein, in the 54 to 558 aa range, expressed in Escherichia coli, with >80%, suitable for SDS-PAGE.

View Alternative Names

GLUD, GLUD1, GDH 1

1 Images
SDS-PAGE - Recombinant Human GLUD1 protein (denatured) (His tag N-Terminus) (AB180318)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human GLUD1 protein (denatured) (His tag N-Terminus) (AB180318)

15% SDS-PAGE analysis of ab180318 (3μg)

Key facts

Purity

>80% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P00367

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSSEAVADREDDPNFFKMVEGFFDRGASIVEDKLVEDLRTRESEEQKRNRVRGILRIIKPCNHVLSLSFPIRRDDGSWEVIEGYRAQHSQHRTPCKGGIRYSTDVSVDEVKALASLMTYKCAVVDVPFGGAKAGVKINPKNYTDNELEKITRRFTMELAKKGFIGPGIDVPAPDMSTGEREMSWIADTYASTIGHYDINAHACVTGKPISQGGIHGRISATGRGVFHGIENFINEASYMSILGMTPGFGDKTFVVQGFGNVGLHSMRYLHRFGAKCIAVGESDGSIWNPDGIDPKELEDFKLQHGSILGFPKAKPYEGSILEADCDILIPAASEKQLTKSNAPRVKAKIIAEGANGPTTPEADKIFLERNIMVIPDLYLNAGGVTVSYFEWLKNLNHVSYGRLTFKYERDSNYHLLMSVQESLERKFGKHGGTIPIVPTAEFQDRISGASEKDIVHSGLAYTMERSARQIMRTAMKYNLGLDLRTAAYVNAIEKVFKVYNEAGVTFTEAVADREDDPNFFKMVEGFFDRGASIVEDKLVEDLRTRESEEQKRNRVRGILRIIKPCNHVLSLSFPIRRDDGSWEVIEGYRAQHSQHRTPCKGGIRYSTDVSVDEVKALASLMTYKCAVVDVPFGGAKAGVKINPKNYTDNELEKITRRFTMELAKKGFIGPGIDVPAPDMSTGEREMSWIADTYASTIGHYDINAHACVTGKPISQGGIHGRISATGRGVFHGIENFINEASYMSILGMTPGFGDKTFVVQGFGNVGLHSMRYLHRFGAKCIAVGESDGSIWNPDGIDPKELEDFKLQHGSILGFPKAKPYEGSILEADCDILIPAASEKQLTKSNAPRVKAKIIAEGANGPTTPEADKIFLERNIMVIPDLYLNAGGVTVSYFEWLKNLNHVSYGRLTFKYERDSNYHLLMSVQESLERKFGKHGGTIPIVPTAEFQDRISGASEKDIVHSGLAYTMERSARQIMRTAMKYNLGLDLRTAAYVNAIEKVFKVYNEAGVTFT","proteinLength":"Full Length","predictedMolecularWeight":"58.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":558,"aminoAcidStart":54,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P00367","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GLUD1 also known as glutamate dehydrogenase 1 is an enzyme that catalyzes the reversible oxidative deamination of glutamate to α-ketoglutarate and ammonia playing an important role in amino acid metabolism. The molecular mass of GLUD1 is approximately 61 kDa. It is expressed in many tissues with notably high levels in liver kidney and neural tissues. GLUD1 is essential for maintaining the balance between amino acid and carbohydrate metabolism.
Biological function summary

GLUD1 facilitates nitrogen and carbon homeostasis through its role in amino acid breakdown and energy metabolism. Although not part of a complex GLUD1's activity impacts central metabolic processes influencing cellular energy status and neurotransmitter regulation. The enzyme contributes to the glutamate-glutamine cycle which is important for neurotransmission in the brain. Its regulation by ADP GDP and other molecules enables cells to adapt to energetic demands.

Pathways

GLUD1 is integral to the tricarboxylic acid (TCA) cycle and urea cycle impacting both energy production and detoxification of ammonia. In the TCA cycle GLUD1 influences the availability of α-ketoglutarate interfacing with enzymes such as isocitrate dehydrogenase. In the urea cycle it aids in converting ammonia to urea in the liver. Additionally GLUD1's function connects with glutamine synthetase in managing amino acid synthesis and degradation.

GLUD1 is associated with congenital hyperinsulinism and hepatic encephalopathy. Hyperinsulinism often results from mutations in GLUD1 which lead to excessive insulin release due to increased leucine sensitivity. In hepatic encephalopathy GLUD1's role in ammonia detoxification becomes important with impaired function potentially leading to elevated ammonia levels and neurological dysfunction. Understanding GLUD1's interactions with other proteins such as those in insulin signaling pathways could further elucidate its impact on these medical conditions.
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Specifications

Form

Liquid

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General info

Function

Mitochondrial glutamate dehydrogenase that catalyzes the conversion of L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle (PubMed : 11032875, PubMed : 11254391, PubMed : 16023112, PubMed : 16959573). Plays a role in insulin homeostasis (PubMed : 11297618, PubMed : 9571255). May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate (By similarity).

Sequence similarities

Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Post-translational modifications

ADP-ribosylated by SIRT4, leading to inactivate glutamate dehydrogenase activity (PubMed:16959573). Stoichiometry shows that ADP-ribosylation occurs in one subunit per catalytically active homohexamer (PubMed:16023112).

Subcellular localisation

Mitochondrion

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Product protocols

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Target data

Mitochondrial glutamate dehydrogenase that catalyzes the conversion of L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle (PubMed : 11032875, PubMed : 11254391, PubMed : 16023112, PubMed : 16959573). Plays a role in insulin homeostasis (PubMed : 11297618, PubMed : 9571255). May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate (By similarity).
See full target information GLUD1
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