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AB132807

Recombinant Human GLUD2 protein (GST tag N-Terminus)

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Recombinant Human GLUD2 protein (GST tag N-Terminus) is a Human Fragment protein, in the 295 to 558 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

GLUDP1, GLUD2, GDH 2

1 Images
SDS-PAGE - Recombinant Human GLUD2 protein (AB132807)
  • SDS-PAGE

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SDS-PAGE - Recombinant Human GLUD2 protein (AB132807)

12.5% SDS-PAGE analysis of ab132807 stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

SDS-PAGE, ELISA, WB

applications

Biologically active

No

Accession

P49448

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MTPGFRDKTFVVQGFGNVGLHSMRYLHRFGAKCIAVGESDGSIWNPDGIDPKELEDFKLQHGSILGFPKAKPYEGSILEVDCDILIPAATEKQLTKSNAPRVKAKIIAEGANGPTTPEADKIFLERNILVIPDLYLNAGGVTVSYFEWLKNLNHVSYGRLTFKYERDSNYHLLLSVQESLERKFGKHGGTIPIVPTAEFQDSISGASEKDIVHSALAYTMERSARQIMHTAMKYNLGLDLRTAAYVNAIEKVFKVYSEAGVTFT","proteinLength":"Fragment","predictedMolecularWeight":"54.78 kDa","actualMolecularWeight":null,"aminoAcidEnd":558,"aminoAcidStart":295,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P49448","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GLUD2 also known as glutamate dehydrogenase 2 plays a critical role in the conversion of glutamate to α-ketoglutarate and ammonia facilitated by the NAD+ or NADP+ cofactor. This enzyme belongs to the family of oxidoreductases and has a molecular mass of around 62 kDa. GLUD2 is mainly expressed in the brain specifically in neurons and glial cells reflecting its significant function in central nervous system activities.
Biological function summary

GLUD2 is involved in amino acid metabolism and neurotransmitter flux. It participates in balancing nitrogen and carbon important for neurotransmitter release and energy production in neural cells. The enzyme often exists as part of signaling complexes within mitochondria emphasizing its role in cellular energy homeostasis. Through its metabolic activities GLUD2 supports synapses in maintaining cognitive functions.

Pathways

GLUD2 plays important roles in the TCA cycle and glutamine-glutamate cycle. In the TCA cycle it facilitates the entry of nitrogen into the cycle via α-ketoglutarate production directly influencing cellular respiration and ATP generation. Its activity in the glutamine-glutamate cycle links it to interactions with glutaminase affecting synaptic signaling and plasticity which are vital for neural communication.

GLUD2 has associations with neurodegenerative conditions and metabolic disorders. Its malfunction contributes to hyperinsulinism-hyperammonemia syndrome and increased risk for certain neuropathologies like Alzheimer's disease. GLUD2's relationship with proteins like glutaminase and other dehydrogenases can influence the progression of these disorders by altering metabolic fluxes.
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Specifications

Form

Liquid

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General info

Function

Important for recycling the chief excitatory neurotransmitter, glutamate, during neurotransmission.

Sequence similarities

Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Post-translational modifications

Stoichiometry shows that ADP-ribosylation occurs in one subunit per catalytically active homohexamer.

Subcellular localisation

Mitochondrion matrix

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Product protocols

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Target data

Important for recycling the chief excitatory neurotransmitter, glutamate, during neurotransmission.
See full target information GLUD2
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Complementary Products

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AB222823

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LEPREL1 antibody (AB222823)

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Primary Antibodies

AB154027

Anti-GLUD1 + GLUD2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody (AB154027)

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SDS-PAGE - Recombinant Human PHAP1 protein (His tag N-Terminus) (AB101155)

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Primary Antibodies

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Anti-PBA1 antibody

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Product promise

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