Recombinant Human GLUD2 protein is a Human Fragment protein, in the 295 to 558 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
Amino acid sequence
M T P G F R D K T F V V Q G F G N V G L H S M R Y L H R F G A K C I A V G E S D G S I W N P D G I D P K E L E D F K L Q H G S I L G F P K A K P Y E G S I L E V D C D I L I P A A T E K Q L T K S N A P R V K A K I I A E G A N G P T T P E A D K I F L E R N I L V I P D L Y L N A G G V T V S Y F E W L K N L N H V S Y G R L T F K Y E R D S N Y H L L L S V Q E S L E R K F G K H G G T I P I V P T A E F Q D S I S G A S E K D I V H S A L A Y T M E R S A R Q I M H T A M K Y N L G L D L R T A A Y V N A I E K V F K V Y S E A G V T F T
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Important for recycling the chief excitatory neurotransmitter, glutamate, during neurotransmission.
Alternative names
GLUDP1, GLUD2, GDH 2
Recommended products
Recombinant Human GLUD2 protein is a Human Fragment protein, in the 295 to 558 aa range, expressed in Wheat germ and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, ELISA, WB
- Biologically active
- No
- Accession
- P49448-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M T P G F R D K T F V V Q G F G N V G L H S M R Y L H R F G A K C I A V G E S D G S I W N P D G I D P K E L E D F K L Q H G S I L G F P K A K P Y E G S I L E V D C D I L I P A A T E K Q L T K S N A P R V K A K I I A E G A N G P T T P E A D K I F L E R N I L V I P D L Y L N A G G V T V S Y F E W L K N L N H V S Y G R L T F K Y E R D S N Y H L L L S V Q E S L E R K F G K H G G T I P I V P T A E F Q D S I S G A S E K D I V H S A L A Y T M E R S A R Q I M H T A M K Y N L G L D L R T A A Y V N A I E K V F K V Y S E A G V T F T
- Accession
- P49448
- Protein length
- Fragment
- Predicted molecular weight
- 54.78 kDa
- Amino acids
- 295 to 558
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Important for recycling the chief excitatory neurotransmitter, glutamate, during neurotransmission.
- Sequence similarities
Belongs to the Glu/Leu/Phe/Val dehydrogenases family.
- Post-translational modifications
Stoichiometry shows that ADP-ribosylation occurs in one subunit per catalytically active homohexamer.
- Subcellular localisation
- Mitochondrion matrix
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GLUD2 also known as glutamate dehydrogenase 2 plays a critical role in the conversion of glutamate to α-ketoglutarate and ammonia facilitated by the NAD+ or NADP+ cofactor. This enzyme belongs to the family of oxidoreductases and has a molecular mass of around 62 kDa. GLUD2 is mainly expressed in the brain specifically in neurons and glial cells reflecting its significant function in central nervous system activities.
- Biological function summary
GLUD2 is involved in amino acid metabolism and neurotransmitter flux. It participates in balancing nitrogen and carbon important for neurotransmitter release and energy production in neural cells. The enzyme often exists as part of signaling complexes within mitochondria emphasizing its role in cellular energy homeostasis. Through its metabolic activities GLUD2 supports synapses in maintaining cognitive functions.
- Pathways
GLUD2 plays important roles in the TCA cycle and glutamine-glutamate cycle. In the TCA cycle it facilitates the entry of nitrogen into the cycle via α-ketoglutarate production directly influencing cellular respiration and ATP generation. Its activity in the glutamine-glutamate cycle links it to interactions with glutaminase affecting synaptic signaling and plasticity which are vital for neural communication.
- Associated diseases and disorders
GLUD2 has associations with neurodegenerative conditions and metabolic disorders. Its malfunction contributes to hyperinsulinism-hyperammonemia syndrome and increased risk for certain neuropathologies like Alzheimer's disease. GLUD2's relationship with proteins like glutaminase and other dehydrogenases can influence the progression of these disorders by altering metabolic fluxes.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human GLUD2 protein (ab132807)
12.5% SDS-PAGE analysis of ab132807 stained with Coomassie Blue.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com