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AB198065

Recombinant human Glutaminase protein (His tag N-Terminus)

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Recombinant human Glutaminase protein (His tag N-Terminus) is a Human Full Length protein, in the 17 to 669 aa range, expressed in Escherichia coli, with >74%, suitable for SDS-PAGE, FuncS.

View Alternative Names

GLS1, KIAA0838, GLS, K-glutaminase, L-glutamine amidohydrolase

2 Images
Functional Studies - Recombinant human Glutaminase protein (His tag N-Terminus) (AB198065)
  • FuncS

Supplier Data

Functional Studies - Recombinant human Glutaminase protein (His tag N-Terminus) (AB198065)

Example of specific activity of ab198065

SDS-PAGE - Recombinant human Glutaminase protein (His tag N-Terminus) (AB198065)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human Glutaminase protein (His tag N-Terminus) (AB198065)

4-20% SDS-PAGE analysis of 4 μg ab198065 with Coomassie staining.

Key facts

Purity

>74% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Specific Activity: ≥ 25 nmol/min/μg

Assay Conditions:
The reaction mixture contained 100 mM Tris, pH 8.6, 150 mM K2HPO4, 25 mM L-glutamine, 2 mM NAD+, 4 units of GDH, and various amounts of ab198065 in a volume of 200 μl. Reactions were initiated with the addition of ab198065, and the increase in NADH absorbance at 340 nm was continuously monitored for 7 minutes at 37°C. Molar extinction coefficient of NADH is 6,220 M-1cm-1.

Accession

O94925

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

storage-buffer

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Complementary Products

Proteins & Peptides

AB107143

Recombinant E. coli glsA1 protein (His tag N-Terminus)

SDS-PAGE - Recombinant E. coli glsA1 protein (His tag N-Terminus) (AB107143)

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Primary Antibodies

AB156876

Anti-Glutaminase antibody [EP7212]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutaminase antibody [EP7212] (AB156876)

  • 4
  • 4
Proteins & Peptides

AB190411

Recombinant human RIP protein (Active)

Functional Studies - Recombinant human RIP protein (Active) (AB190411)

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Assay Kits

AB284547

Glutaminase (GLS) Activity Assay Kit (Fluorometric)

Functional Studies - Glutaminase (GLS) Activity Assay Kit (Fluorometric) (AB284547)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MHHHHHHSPAGVSATLRRAQPLVTLCRRPRGGGRPAAGPAAAARLHPWWGGGGWPAEPLARGLSSSPSEILQELGKGSTHPQPGVSPPAAPAAPGPKDGPGETDAFGNSEGKELVASGENKIKQGLLPSLEDLLFYTIAEGQEKIPVHKFITALKSTGLRTSDPRLKECMDMLRLTLQTTSDGVMLDKDLFKKCVQSNIVLLTQAFRRKFVIPDFMSFTSHIDELYESAKKQSGGKVADYIPQLAKFSPDLWGVSVCTVDGQRHSTGDTKVPFCLQSCVKPLKYAIAVNDLGTEYVHRYVGKEPSGLRFNKLFLNEDDKPHNPMVNAGAIVVTSLIKQGVNNAEKFDYVMQFLNKMAGNEYVGFSNATFQSERESGDRNFAIGYYLKEKKCFPEGTDMVGILDFYFQLCSIEVTCESASVMAATLANGGFCPITGERVLSPEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSGVAGGILLVVPNVMGMMCWSPPLDKMGNSVKGIHFCHDLVSLCNFHNYDNLRHFAKKLDPRREGGDQRVKSVINLLFAAYTGDVSALRRFALSAMDMEQRDYDSRTALHVAAAEGHVEVVKFLLEACKVNPFPKDRWNNTPMDEALHFGHHDVFKILQEYQVQYTPQGDSDNGKENQTVHKNLDGLL","proteinLength":"Full Length","predictedMolecularWeight":"72.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":669,"aminoAcidStart":17,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O94925","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glutaminase also known as GLS or GLS1 is an enzyme that catalyzes the conversion of glutamine to glutamate an important step in cellular nitrogen and energy metabolism. This enzyme weighs approximately 65 kDa and is expressed in various tissues particularly in the brain kidney and liver. Glutaminase exists in two isoforms GLS1 and GLS2 with GLS1 being the more studied due to its role in cancer metabolism.
Biological function summary

Glutaminase plays a central role in cellular processes by facilitating the production of glutamate which is an important neurotransmitter in the brain and a precursor for the synthesis of gamma-aminobutyric acid (GABA). It is not part of a larger complex but acts independently in the mitochondrial matrix where it performs the glutaminase reaction. The activity of this enzyme is monitored closely because it affects multiple cellular functions and has implications in many diseases.

Pathways

Glutaminase is instrumental in the glutaminolysis and tricarboxylic acid (TCA) cycles. These pathways are essential for cellular energy production and biosynthesis. GLS1 activity influences other proteins such as alanine transaminase (ALT) and aspartate transaminase (AST) which also play roles in amino acid metabolism and energy production. Glutaminase inhibitors have been explored as potential therapeutic agents by disrupting the glutaminolysis pathway in cancer cells leading to decreased tumor growth.

Glutaminase has a significant link to cancer and neurodegenerative diseases. In cancer GLS1 activity is often upregulated as tumor cells require increased glutamine metabolism for growth and survival making GLS1 inhibitors a target in cancer therapy. Neurodegenerative disorders such as Alzheimer's disease also show altered glutamate levels implicating glutaminase in disease progression. These disorders highlight the enzyme's potential connections with proteins like NMDA receptors in the brain where glutamate acts as a neurotransmitter affecting synaptic activity.
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Specifications

Form

Liquid

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General info

Function

Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate, the main excitatory neurotransmitter in the brain (PubMed : 30239721, PubMed : 30575854, PubMed : 30970188).. Isoform 2. Lacks catalytic activity.

Sequence similarities

Belongs to the glutaminase family.

Post-translational modifications

Synthesized as a 74-kDa cytosolic precursor which is proteolytically processed by the mitochondrial-processing peptidase (MPP) via a 72-kDa intermediate to yield the mature mitochondrial 68- and 65-kDa subunits.

Subcellular localisation

Mitochondrion

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Product protocols

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Target data

Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate, the main excitatory neurotransmitter in the brain (PubMed : 30239721, PubMed : 30575854, PubMed : 30970188).. Isoform 2. Lacks catalytic activity.
See full target information GLS
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