Recombinant human Glutaminase protein is a Human Full Length protein, in the 17 to 669 aa range, expressed in Escherichia coli, with >=74% purity and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Purity
- >=74% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M H H H H H H S P A G V S A T L R R A Q P L V T L C R R P R G G G R P A A G P A A A A R L H P W W G G G G W P A E P L A R G L S S S P S E I L Q E L G K G S T H P Q P G V S P P A A P A A P G P K D G P G E T D A F G N S E G K E L V A S G E N K I K Q G L L P S L E D L L F Y T I A E G Q E K I P V H K F I T A L K S T G L R T S D P R L K E C M D M L R L T L Q T T S D G V M L D K D L F K K C V Q S N I V L L T Q A F R R K F V I P D F M S F T S H I D E L Y E S A K K Q S G G K V A D Y I P Q L A K F S P D L W G V S V C T V D G Q R H S T G D T K V P F C L Q S C V K P L K Y A I A V N D L G T E Y V H R Y V G K E P S G L R F N K L F L N E D D K P H N P M V N A G A I V V T S L I K Q G V N N A E K F D Y V M Q F L N K M A G N E Y V G F S N A T F Q S E R E S G D R N F A I G Y Y L K E K K C F P E G T D M V G I L D F Y F Q L C S I E V T C E S A S V M A A T L A N G G F C P I T G E R V L S P E A V R N T L S L M H S C G M Y D F S G Q F A F H V G L P A K S G V A G G I L L V V P N V M G M M C W S P P L D K M G N S V K G I H F C H D L V S L C N F H N Y D N L R H F A K K L D P R R E G G D Q R V K S V I N L L F A A Y T G D V S A L R R F A L S A M D M E Q R D Y D S R T A L H V A A A E G H V E V V K F L L E A C K V N P F P K D R W N N T P M D E A L H F G H H D V F K I L Q E Y Q V Q Y T P Q G D S D N G K E N Q T V H K N L D G L L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate, the main excitatory neurotransmitter in the brain (PubMed:30239721, PubMed:30575854, PubMed:30970188). Isoform 2. Lacks catalytic activity.
Alternative names
GLS1, KIAA0838, GLS, K-glutaminase, L-glutamine amidohydrolase
Recommended products
Recombinant human Glutaminase protein is a Human Full Length protein, in the 17 to 669 aa range, expressed in Escherichia coli, with >=74% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >=74% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
Specific Activity: ≥ 25 nmol/min/μg
Assay Conditions:
The reaction mixture contained 100 mM Tris, pH 8.6, 150 mM K2HPO4, 25 mM L-glutamine, 2 mM NAD+, 4 units of GDH, and various amounts of ab198065 in a volume of 200 μl. Reactions were initiated with the addition of ab198065, and the increase in NADH absorbance at 340 nm was continuously monitored for 7 minutes at 37°C. Molar extinction coefficient of NADH is 6,220 M-1cm-1.- Accession
- O94925-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- M H H H H H H S P A G V S A T L R R A Q P L V T L C R R P R G G G R P A A G P A A A A R L H P W W G G G G W P A E P L A R G L S S S P S E I L Q E L G K G S T H P Q P G V S P P A A P A A P G P K D G P G E T D A F G N S E G K E L V A S G E N K I K Q G L L P S L E D L L F Y T I A E G Q E K I P V H K F I T A L K S T G L R T S D P R L K E C M D M L R L T L Q T T S D G V M L D K D L F K K C V Q S N I V L L T Q A F R R K F V I P D F M S F T S H I D E L Y E S A K K Q S G G K V A D Y I P Q L A K F S P D L W G V S V C T V D G Q R H S T G D T K V P F C L Q S C V K P L K Y A I A V N D L G T E Y V H R Y V G K E P S G L R F N K L F L N E D D K P H N P M V N A G A I V V T S L I K Q G V N N A E K F D Y V M Q F L N K M A G N E Y V G F S N A T F Q S E R E S G D R N F A I G Y Y L K E K K C F P E G T D M V G I L D F Y F Q L C S I E V T C E S A S V M A A T L A N G G F C P I T G E R V L S P E A V R N T L S L M H S C G M Y D F S G Q F A F H V G L P A K S G V A G G I L L V V P N V M G M M C W S P P L D K M G N S V K G I H F C H D L V S L C N F H N Y D N L R H F A K K L D P R R E G G D Q R V K S V I N L L F A A Y T G D V S A L R R F A L S A M D M E Q R D Y D S R T A L H V A A A E G H V E V V K F L L E A C K V N P F P K D R W N N T P M D E A L H F G H H D V F K I L Q E Y Q V Q Y T P Q G D S D N G K E N Q T V H K N L D G L L
- Accession
- O94925
- Protein length
- Full Length
- Predicted molecular weight
- 72.5 kDa
- Amino acids
- 17 to 669
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate, the main excitatory neurotransmitter in the brain (PubMed:30239721, PubMed:30575854, PubMed:30970188).
- Sequence similarities
Belongs to the glutaminase family.
- Post-translational modifications
Synthesized as a 74-kDa cytosolic precursor which is proteolytically processed by the mitochondrial-processing peptidase (MPP) via a 72-kDa intermediate to yield the mature mitochondrial 68- and 65-kDa subunits.
- Subcellular localisation
- Mitochondrion, Mitochondrion matrix
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glutaminase also known as GLS or GLS1 is an enzyme that catalyzes the conversion of glutamine to glutamate an important step in cellular nitrogen and energy metabolism. This enzyme weighs approximately 65 kDa and is expressed in various tissues particularly in the brain kidney and liver. Glutaminase exists in two isoforms GLS1 and GLS2 with GLS1 being the more studied due to its role in cancer metabolism.
- Biological function summary
Glutaminase plays a central role in cellular processes by facilitating the production of glutamate which is an important neurotransmitter in the brain and a precursor for the synthesis of gamma-aminobutyric acid (GABA). It is not part of a larger complex but acts independently in the mitochondrial matrix where it performs the glutaminase reaction. The activity of this enzyme is monitored closely because it affects multiple cellular functions and has implications in many diseases.
- Pathways
Glutaminase is instrumental in the glutaminolysis and tricarboxylic acid (TCA) cycles. These pathways are essential for cellular energy production and biosynthesis. GLS1 activity influences other proteins such as alanine transaminase (ALT) and aspartate transaminase (AST) which also play roles in amino acid metabolism and energy production. Glutaminase inhibitors have been explored as potential therapeutic agents by disrupting the glutaminolysis pathway in cancer cells leading to decreased tumor growth.
- Associated diseases and disorders
Glutaminase has a significant link to cancer and neurodegenerative diseases. In cancer GLS1 activity is often upregulated as tumor cells require increased glutamine metabolism for growth and survival making GLS1 inhibitors a target in cancer therapy. Neurodegenerative disorders such as Alzheimer's disease also show altered glutamate levels implicating glutaminase in disease progression. These disorders highlight the enzyme's potential connections with proteins like NMDA receptors in the brain where glutamate acts as a neurotransmitter affecting synaptic activity.
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2 product images
Functional Studies - Recombinant human Glutaminase protein (ab198065)
Example of specific activity of ab198065
SDS-PAGE - Recombinant human Glutaminase protein (ab198065)
4-20% SDS-PAGE analysis of 4 μg ab198065 with Coomassie staining.
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