Recombinant human Glutamine Synthetase protein is a Human Full Length protein, in the 1 to 373 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M T T S A S S H L N K G I K Q V Y M S L P Q G E K V Q A M Y I W I D G T G E G L R C K T R T L D S E P K C V E E L P E W N F D G S S T L Q S E G S N S D M Y L V P A A M F R D P F R K D P N K L V L C E V F K Y N R R P A E T N L R H T C K R I M D M V S N Q H P W F G M E Q E Y T L M G T D G H P F G W P S N G F P G P Q G P Y Y C G V G A D R A Y G R D I V E A H Y R A C L Y A G V K I A G T N A E V M P A Q W E F Q I G P C E G I S M G D H L W V A R F I L H R V C E D F G V I A T F D P K P I P G N W N G A G C H T N F S T K A M R E E N G L K Y I E E A I E K L S K R H Q Y H I R A Y D P K G G L D N A R R L T G F H E T S N I N D F S A G V A N R S A S I R I P R T V G Q E K K G Y F E D R R P S A N C D P F S V T E A L I R T C L L N E T G D E P F Q Y K N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Glutamine synthetase that catalyzes the ATP-dependent conversion of glutamate and ammonia to glutamine (PubMed:16267323, PubMed:30158707, PubMed:36289327). Its role depends on tissue localization: in the brain, it regulates the levels of toxic ammonia and converts neurotoxic glutamate to harmless glutamine, whereas in the liver, it is one of the enzymes responsible for the removal of ammonia (By similarity). Essential for proliferation of fetal skin fibroblasts (PubMed:18662667). Independently of its glutamine synthetase activity, required for endothelial cell migration during vascular development: acts by regulating membrane localization and activation of the GTPase RHOJ, possibly by promoting RHOJ palmitoylation (PubMed:30158707). May act as a palmitoyltransferase for RHOJ: able to autopalmitoylate and then transfer the palmitoyl group to RHOJ (PubMed:30158707). Plays a role in ribosomal 40S subunit biogenesis (PubMed:26711351). Through the interaction with BEST2, inhibits BEST2 channel activity by affecting the gating at the aperture in the absence of intracellular L-glutamate, but sensitizes BEST2 to intracellular L-glutamate, which promotes the opening of BEST2 and thus relieves its inhibitory effect on BEST2 (PubMed:36289327).
Alternative names
GLNS, GLUL, Glutamine synthetase, GS, Glutamate--ammonia ligase, Palmitoyltransferase GLUL
Recommended products
Recombinant human Glutamine Synthetase protein is a Human Full Length protein, in the 1 to 373 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Specific activity is > 2.000 pmol/min/ug, and is defined as the amount of enzyme that convert L-glutamate to L-glutamine per miunte at pH 7.5 at 37C in coupled system with PK/LDH.
- Accession
- P15104-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF
Sequence info
Amino acid sequence
- M T T S A S S H L N K G I K Q V Y M S L P Q G E K V Q A M Y I W I D G T G E G L R C K T R T L D S E P K C V E E L P E W N F D G S S T L Q S E G S N S D M Y L V P A A M F R D P F R K D P N K L V L C E V F K Y N R R P A E T N L R H T C K R I M D M V S N Q H P W F G M E Q E Y T L M G T D G H P F G W P S N G F P G P Q G P Y Y C G V G A D R A Y G R D I V E A H Y R A C L Y A G V K I A G T N A E V M P A Q W E F Q I G P C E G I S M G D H L W V A R F I L H R V C E D F G V I A T F D P K P I P G N W N G A G C H T N F S T K A M R E E N G L K Y I E E A I E K L S K R H Q Y H I R A Y D P K G G L D N A R R L T G F H E T S N I N D F S A G V A N R S A S I R I P R T V G Q E K K G Y F E D R R P S A N C D P F S V T E A L I R T C L L N E T G D E P F Q Y K N
- Accession
- P15104
- Protein length
- Full Length
- Predicted molecular weight
- 42 kDa
- Amino acids
- 1 to 373
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
Purified by conventional chromatography techniques.
General info
- Function
Glutamine synthetase that catalyzes the ATP-dependent conversion of glutamate and ammonia to glutamine (PubMed:16267323, PubMed:30158707, PubMed:36289327). Its role depends on tissue localization: in the brain, it regulates the levels of toxic ammonia and converts neurotoxic glutamate to harmless glutamine, whereas in the liver, it is one of the enzymes responsible for the removal of ammonia (By similarity). Essential for proliferation of fetal skin fibroblasts (PubMed:18662667). Independently of its glutamine synthetase activity, required for endothelial cell migration during vascular development: acts by regulating membrane localization and activation of the GTPase RHOJ, possibly by promoting RHOJ palmitoylation (PubMed:30158707). May act as a palmitoyltransferase for RHOJ: able to autopalmitoylate and then transfer the palmitoyl group to RHOJ (PubMed:30158707). Plays a role in ribosomal 40S subunit biogenesis (PubMed:26711351). Through the interaction with BEST2, inhibits BEST2 channel activity by affecting the gating at the aperture in the absence of intracellular L-glutamate, but sensitizes BEST2 to intracellular L-glutamate, which promotes the opening of BEST2 and thus relieves its inhibitory effect on BEST2 (PubMed:36289327).
- Sequence similarities
Belongs to the glutamine synthetase family.
- Post-translational modifications
Acetylated by EP300/p300; acetylation is stimulated by increased glutamine levels and promotes ubiquitin-mediated proteasomal degradation.
- Subcellular localisation
- Mitochondrion
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glutamine synthetase also known as glutamine s synthetase or glnA is an enzyme that catalyzes the ATP-dependent conversion of glutamate and ammonia into glutamine. This reaction plays an important role in nitrogen metabolism. Glutamine synthetase has a molecular weight of approximately 620 kDa and forms a multimeric structure commonly seen in bacteria plants and animal tissues with significant expression in the brain liver and kidneys.
- Biological function summary
This enzyme supports the detoxification of ammonia by incorporating it into glutamine an essential amino acid and nitrogen donor. Glutamine synthetase operates independently rather than as part of a larger protein complex. It assists in maintaining cellular nitrogen balance and facilitates the synthesis of proteins and other nitrogen-containing molecules. Glutamine peptides serve vital roles in cellular processes underlining the significance of their synthesis.
- Pathways
Glutamine synthetase integrates into the glutamate and glutamine cycle between neurons and glial cells highlighting its part in neurotransmitter metabolism. It also features prominently in the urea cycle influencing nitrogen disposal in organisms. Glutamine synthetase interacts with glutaminase which assists in transforming glutamine back to glutamate maintaining a balance of nitrogenous compounds within these pathways.
- Associated diseases and disorders
Glutamine synthetase abnormalities link to hepatic encephalopathy and neurodegenerative disorders such as Alzheimer's disease. Altered enzyme expression contributes to increased ammonia levels adversely affecting brain function. In Alzheimer's disease connections with tau and amyloid-beta proteins suggest a link between glutamine synthetase activity and neurotoxic events. Understanding these interactions may offer insights into therapeutic approaches for these conditions.
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SDS-PAGE - Recombinant human Glutamine Synthetase protein (ab222354)
Recombinant human Glutamine synthetase (3μg) analysed by 15% SDS-PAGE.
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