AB86987

Recombinant Human Glutaredoxin 1 protein (Tag Free)

Name: Recombinant Human Glutaredoxin 1 protein (Tag Free) (ab86987) | Abcam
Brand: Abcam Limited
SKU: AB86987

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(1 Publication)

Recombinant Human Glutaredoxin 1 protein (Tag Free) is a Human Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, WB.

View Alternative Names

GRX, GLRX, Glutaredoxin-1, Thioltransferase-1, TTase-1

3 Images

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Western blot - Recombinant Human Glutaredoxin 1 protein (Tag Free) (AB86987)

All lanes:

Western blot - Anti-Glutaredoxin 1 antibody (<a href='/en-us/products/primary-antibodies/glutaredoxin-1-antibody-ab45953'>ab45953</a>) at 1/250 dilution

Lane 1:

Western blot - Recombinant Human Glutaredoxin 1 protein (Tag Free) (ab86987) at 0.01 µg

Lane 2:

Western blot - Recombinant Human Glutaredoxin 1 protein (Tag Free) (ab86987) at 0.001 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 11 kDa

true

Exposure time: 1min

Unknown

Western blot - Recombinant Human Glutaredoxin 1 protein (Tag Free) (AB86987)

All lanes:

Western blot - Anti-Glutaredoxin 1 antibody (<a href='/en-us/products/primary-antibodies/glutaredoxin-1-antibody-ab45953'>ab45953</a>) at 1/250 dilution

Lane 1:

Western blot - Recombinant Human Glutaredoxin 1 protein (Tag Free) (ab86987) at 0.01 µg

Lane 2:

Western blot - Recombinant Human Glutaredoxin 1 protein (Tag Free) (ab86987) at 0.001 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 11 kDa

true

Exposure time: 1min

SDS-PAGE - Recombinant Human Glutaredoxin 1 protein (AB86987)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Glutaredoxin 1 protein (AB86987)

3ug by SDS-PAGE under reducing conditions and visualized by coomassie blue stain.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Applications

WB, SDS-PAGE

applications

Biologically active

Accession

P35754

Animal free

Carrier free

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab86987 can be used as a WB positive control in conjunction with <a href='/en-us/products/primary-antibodies/glutaredoxin-1-antibody-ab45953'>ab45953</a>.</p>" } } }

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Sequence info

[{"sequence":"MAQEFVNCKIQPGKVVVFIKPTCPYCRRAQEILSQLPIKQGLLEFVDITATNHTNEIQDYLQQLTGARTVPRVFIGKDCIGGCSDLVSLQQSGELLTRLKQIGALQ","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P35754","tags":[]}]

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

False

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glutaredoxin 1 also known as GLRX or thioltransferase is a small protein with a mass of around 12 kDa. It functions mechanically by catalyzing the reduction of disulfide bonds in proteins using glutathione as a cofactor. This enzyme plays a critical role in maintaining the redox balance within cells. Glutaredoxin 1 is widely expressed in various tissues with particularly high levels in the liver and brain. Its ubiquitous expression highlights its importance in cellular processes requiring reduction-oxidation reactions.

Biological function summary

This enzyme acts as an important regulator in cellular redox homeostasis and defense against oxidative stress. Glutaredoxin 1 does not technically form a complex but often interacts with multiple other proteins to modulate their redox state. It is involved in the regulation of apoptosis signaling pathways and the repair of oxidatively damaged proteins. Through these actions Glutaredoxin 1 protects cells from damage and maintains proper cellular function.

Pathways

Glutaredoxin 1 participates in key processes like the regulation of the thiol-disulfide balance and the glutathione metabolism pathway. These pathways are important for cellular detoxification and antioxidant defense. It works closely with glutathione peroxidase to mitigate oxidative damage and preserves cellular integrity by ensuring reduced forms of cysteine residues on proteins. This function links Glutaredoxin 1 to other proteins such as thioredoxin which shares similar roles in oxidative stress management.

Glutaredoxin 1 plays a significant part in neurodegenerative diseases and cardiovascular disorders. Its role in managing oxidative stress links it to conditions such as Alzheimer's disease where oxidative damage is a known contributor to disease progression. The relationship between Glutaredoxin 1 and proteins like amyloid-beta prevalent in Alzheimer's further highlights its importance. In cardiovascular disorders Glutaredoxin 1 influences the function of endothelial nitric oxide synthase a protein important for vascular health suggesting its involvement in maintaining vascular function and preventing cardiovascular diseases.

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Specifications

Form

Liquid

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General info

Function

Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins.

Sequence similarities

Belongs to the glutaredoxin family.

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins.

See full target information GLRX

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell chemical biology 26:366-377.e12 PubMed30661989

2019

Frenolicin B Targets Peroxiredoxin 1 and Glutaredoxin 3 to Trigger ROS/4E-BP1-Mediated Antitumor Effects.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Ye,Yinan Zhang,Yanan Cao,Xiachang Wang,Yubin Guo,Jing Chen,Jamie Horn,Larissa V Ponomareva,Luksana Chaiswing,Khaled A Shaaban,Qiou Wei,Bradley D Anderson,Daret K St Clair,Haining Zhu,Markos Leggas,Jon S Thorson,Qing-Bai She

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Recombinant Human Glutaredoxin 1 protein (Tag Free)

Western blot - Recombinant Human Glutaredoxin 1 protein (Tag Free) (AB86987)

Western blot - Recombinant Human Glutaredoxin 1 protein (Tag Free) (AB86987)

SDS-PAGE - Recombinant Human Glutaredoxin 1 protein (AB86987)

Key facts

Purity

Expression system

Tags

Applications

Biologically active

Accession

Animal free

Carrier free

Species

Storage buffer

Reactivity data

Sequence info

Properties and storage information

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Specifications

Form

General info

Function

Sequence similarities

Product protocols

Target data

Publications (1)

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Primary Antibodies

Primary Antibodies

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