Recombinant Human Glutaredoxin 5 protein is a Human Full Length protein, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
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- Cell chemical biology 26:366-377.e122019Frenolicin B Targets Peroxiredoxin 1 and Glutaredoxin 3 to Trigger ROS/4E-BP1-Mediated Antitumor Effects.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQing Ye,Yinan Zhang,Yanan Cao,Xiachang Wang,Yubin Guo,Jing Chen,Jamie Horn,Larissa V Ponomareva,Luksana Chaiswing,Khaled A Shaaban,Qiou Wei,Bradley D Anderson,Daret K St Clair,Haining Zhu,Markos Leggas,Jon S Thorson,Qing-Bai ShePubMed 30661989
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M S G S L G R A A A A L L R W G R G A G G G G L W G P G V R A A G S G A G G G G S A E Q L D A L V K K D K V V V F L K G T P E Q P Q C G F S N A V V Q I L R L H G V R D Y A A Y N V L D D P E L R Q G I K D Y S N W P T I P Q V Y L N G E F V G G C D I L L Q M H Q N G D L V E E L K K L G I H S A L L D E K K D Q D S K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Monothiol glutaredoxin involved in mitochondrial iron-sulfur (Fe/S) cluster transfer (PubMed:20364084, PubMed:23615440). Receives 2Fe/2S clusters from scaffold protein ISCU and mediates their transfer to apoproteins, to the 4Fe/FS cluster biosynthesis machinery, or export from mitochondrion (PubMed:20364084, PubMed:23615440, PubMed:24334290). Required for normal regulation of hemoglobin synthesis by the iron-sulfur protein ACO1 (PubMed:20364084).
Alternative names
C14orf87, GLRX5, Monothiol glutaredoxin-5
Recommended products
Recombinant Human Glutaredoxin 5 protein is a Human Full Length protein, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q86SX6-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M S G S L G R A A A A L L R W G R G A G G G G L W G P G V R A A G S G A G G G G S A E Q L D A L V K K D K V V V F L K G T P E Q P Q C G F S N A V V Q I L R L H G V R D Y A A Y N V L D D P E L R Q G I K D Y S N W P T I P Q V Y L N G E F V G G C D I L L Q M H Q N G D L V E E L K K L G I H S A L L D E K K D Q D S K
- Accession
- Q86SX6
- Protein length
- Full Length
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
Purified using conventional chromatography techniques.
General info
- Function
Monothiol glutaredoxin involved in mitochondrial iron-sulfur (Fe/S) cluster transfer (PubMed:20364084, PubMed:23615440). Receives 2Fe/2S clusters from scaffold protein ISCU and mediates their transfer to apoproteins, to the 4Fe/FS cluster biosynthesis machinery, or export from mitochondrion (PubMed:20364084, PubMed:23615440, PubMed:24334290). Required for normal regulation of hemoglobin synthesis by the iron-sulfur protein ACO1 (PubMed:20364084).
- Sequence similarities
Belongs to the glutaredoxin family. Monothiol subfamily.
- Subcellular localisation
- Mitochondrion matrix
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glutaredoxin 5 also known as GRX5 is a mitochondrial protein with a molecular mass of approximately 17 kDa. It efficiently catalyzes the reduction of disulfide bonds in substrate proteins through a thiol-disulfide exchange mechanism. GRX5 is highly expressed in tissues with active metabolism such as heart liver and skeletal muscles. This protein belongs to the glutaredoxin family which are small redox enzymes that use glutathione as a cofactor.
- Biological function summary
The function of GRX5 revolves around iron-sulfur (Fe-S) cluster assembly. It is part of a multiprotein complex involved in the maturation of Fe-S proteins within the mitochondria. These iron-sulfur clusters are essential for various cellular processes including enzymatic functions and electron transport. The loss of GRX5 activity disrupts Fe-S cluster assembly leading to compromised cellular function and increased oxidative stress.
- Pathways
GRX5 plays a significant role in the biosynthesis of Fe-S clusters. It is closely connected to the ISC assembly machinery an important pathway for the formation of these clusters. In this context GRX5 interacts with proteins such as ISCU and NFU1 contributing to the proper maturation and stability of Fe-S proteins which are pivotal for mitochondrial electron transport chain function and DNA repair mechanisms.
- Associated diseases and disorders
GRX5 dysregulation is linked to autosomal recessive sideroblastic anemia and mitochondrial dysfunction. This disorder results from defective Fe-S cluster biosynthesis leading to impaired haem synthesis and mitochondrial iron accumulation. GRX5's interaction with frataxin another protein involved in Fe-S cluster assembly further elucidates its role in the pathophysiology of diseases like Friedreich's ataxia where deficient iron-sulfur cluster synthesis leads to neurodegeneration and systemic abnormalities.
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1 product image
SDS-PAGE - Recombinant Human Glutaredoxin 5 protein (ab95352)
15% SDS-PAGE showing ab95352 at approximately 19 kDa (3 μg).
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