Recombinant Human Glutathione Peroxidase 3/GPx-3 protein is a Human Full Length protein, in the 21 to 226 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M Q S R G Q E K S K M D C H G G I S G T I Y E Y G A L T I D G E E Y I P F K Q Y A G K Y V L F V N V A S Y C G L T G Q Y I E L N A L Q E E L A P F G L V I L G F P C N Q F G K Q E P G E N S E I L P T L K Y V R P G G G F V P N F Q L F E K G D V N G E K E Q K F Y T F L K N S C P P T S E L L G T S D R L F W E P M K V H D I R W N F E K F L V G P D G I P I M R W H H R T T V S N V K M D I L S Y M R R Q A A L G V K R K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione.
Alternative names
GPXP, GPX3, Glutathione peroxidase 3, GPx-3, GSHPx-3, Extracellular glutathione peroxidase, Plasma glutathione peroxidase, GPx-P, GSHPx-P
Recommended products
Recombinant Human Glutathione Peroxidase 3/GPx-3 protein is a Human Full Length protein, in the 21 to 226 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P22352-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 40% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M Q S R G Q E K S K M D C H G G I S G T I Y E Y G A L T I D G E E Y I P F K Q Y A G K Y V L F V N V A S Y C G L T G Q Y I E L N A L Q E E L A P F G L V I L G F P C N Q F G K Q E P G E N S E I L P T L K Y V R P G G G F V P N F Q L F E K G D V N G E K E Q K F Y T F L K N S C P P T S E L L G T S D R L F W E P M K V H D I R W N F E K F L V G P D G I P I M R W H H R T T V S N V K M D I L S Y M R R Q A A L G V K R K
- Accession
- P22352
- Protein length
- Full Length
- Predicted molecular weight
- 25.7 kDa
- Amino acids
- 21 to 226
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab123158 was purified using conventional chromatography and is >85% pure by SDS PAGE.
General info
- Function
Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione.
- Sequence similarities
Belongs to the glutathione peroxidase family.
- Post-translational modifications
The N-terminus is blocked.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Previously labelled as Glutathione Peroxidase 3.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glutathione Peroxidase 3 also known as GPx-3 or 3GPx is an antioxidant enzyme important for protecting cells from oxidative damage by reducing hydrogen peroxide and organic hydroperoxides. It is a member of the glutathione peroxidase family and has a molecular mass of approximately 23 kDa. GPx-3 is expressed mainly in the kidney and subsequently released into the bloodstream playing an important role in its extracellular antioxidant defense mechanism.
- Biological function summary
GPx-3 helps maintain cellular redox balance and is involved in detoxifying harmful oxidative agents. It does not form part of any larger complex but works independently to reduce peroxides with the help of glutathione an important reducing agent. GPx-3 has significant activities in plasma and extracellular fluids indicating its role in protecting the body from oxidative stress away from cellular environments.
- Pathways
GPx-3 functions in the glutathione metabolic pathway and the defense system against oxidative stress. It works together with proteins such as glutathione reductase to recycle oxidized glutathione back to its reduced form allowing continuous protection against reactive oxygen species. This pathway is essential for maintaining balance between oxidative stress and antioxidant defense in the organism.
- Associated diseases and disorders
GPx-3 has been linked to cardiovascular diseases and certain types of cancer. Low levels of GPx-3 in plasma have been associated with increased risk of thrombosis as antioxidant defenses are compromised. In cancer studies altered GPx-3 expression is often observed thought to affect the progression of disease by changing the cellular redox environment. Other proteins that interact with GPx-3 during disease conditions include thioredoxin and catalase both influencing and being regulated by redox states in diseased tissues.
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1 product image
SDS-PAGE - Recombinant Human Glutathione Peroxidase 3/GPx-3 protein (ab123158)
15% SDS-PAGE analysis of 3 μg of Human Glutathione Peroxidase 3/GPx-3 protein (ab123158).
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