Recombinant Human Glutathione Peroxidase 4 Protein Standard (His tag)
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Recombinant Human Glutathione Peroxidase 4 Protein Standard (His tag) is a Human Fragment protein, expressed in Escherichia coli, with >80%, suitable for SDS-PAGE, sELISA.
View Alternative Names
Phospholipid hydroperoxide glutathione peroxidase GPX4, PHGPx, Glutathione peroxidase 4, GPx-4, GSHPx-4, GPX4
- sELISA
Supplier Data
Sandwich ELISA - Recombinant Human Glutathione Peroxidase 4 Protein Standard (His tag) (AB316572)
Sandwich ELISA with the capture antibody dilution at 2 ug/mL and detector antibody dilution at 0.5 ug/mL.
- SDS-PAGE
Supplier Data
SDS-PAGE - Recombinant Human Glutathione Peroxidase 4 Protein Standard (His tag) (AB316572)
SDS-PAGE analysis of ab316572 under reducing conditions for 2ug protein.
Complementary Products
Reagents, Controls & Accessories
AB313996
Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa)
- 0
- 0
Primary Antibodies
AB125066
Anti-Glutathione Peroxidase 4 antibody [EPNCIR144]
BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (AB125066)](https://content.abcam.com/products/images/glutathione-peroxidase-4-antibody-epncir144-ab125066--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img436422.jpg)
- 5
- 13
Reactivity data
Product details
While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.
Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.
The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Support team to know which antibody pair is suitable for this protein.
Sequence info
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
GPX4 plays an important role in protecting cellular membranes from lipid peroxidation by acting alone which differentiates it from other glutathione peroxidases that work in a complex. It is necessary for the production of glutathione products and helps maintain redox homeostasis by converting glutathione peroxides. This action prevents cell death pathways linked to oxidative stress and ensures cellular integrity particularly in sensitive tissues like the brain and reproductive organs.
Pathways
GPX4 integrates into important cellular antioxidant pathways. Specifically it functions within the glutathione metabolic pathway collaborating with related proteins like glutathione synthetase and glutathione reductase. GPX4 is also involved in ferroptosis regulation a cell death pathway critically linked to glutathione levels. It helps balance redox-sensitive signaling and metabolic activities demonstrating synergy with other antioxidant enzymes in the cellular response to oxidative stress.
Specifications
Form
Liquid
General info
Function
Essential antioxidant peroxidase that directly reduces phospholipid hydroperoxide even if they are incorporated in membranes and lipoproteins (PubMed : 40281343). Can also reduce cholesterol hydroperoxide and thymine hydroperoxide (By similarity). Plays a key role in protecting cells from oxidative damage by preventing membrane lipid peroxidation (PubMed : 40281343). Required to prevent cells from ferroptosis, a non-apoptotic cell death resulting from an iron-dependent accumulation of lipid reactive oxygen species (PubMed : 24439385, PubMed : 40281343). The presence of selenocysteine (Sec) versus Cys at the active site is essential for life : it provides resistance to overoxidation and prevents cells against ferroptosis (By similarity). The presence of Sec at the active site is also essential for the survival of a specific type of parvalbumin-positive interneurons, thereby preventing against fatal epileptic seizures (By similarity). May be required to protect cells from the toxicity of ingested lipid hydroperoxides (By similarity). Required for normal sperm development and male fertility (By similarity). Essential for maturation and survival of photoreceptor cells (By similarity). Plays a role in a primary T-cell response to viral and parasitic infection by protecting T-cells from ferroptosis and by supporting T-cell expansion (By similarity). Plays a role of glutathione peroxidase in platelets in the arachidonic acid metabolism (PubMed : 11115402). Reduces hydroperoxy ester lipids formed by a 15-lipoxygenase that may play a role as down-regulator of the cellular 15-lipoxygenase pathway (By similarity). Can reduce fatty acid-derived hydroperoxides (PubMed : 11115402, PubMed : 36608588). Can also reduce small soluble hydroperoxides such as H2O2, cumene hydroperoxide and tert-butyl hydroperoxide (PubMed : 17630701, PubMed : 36608588).
Sequence similarities
Belongs to the glutathione peroxidase family.
Post-translational modifications
Degraded via chaperone-mediated autophagy, promoting ferroptosis.. Hydroxylation by EGLN3/PHD3 promotes its stabilization by preventing degradation via chaperone-mediated autophagy.
Target data
Product promise
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