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AB109928

Recombinant Human GLYAT/GAT protein

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Recombinant Human GLYAT/GAT protein is a Human Full Length protein, in the 1 to 296 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

ACGNAT, CAT, GAT, GLYAT, Glycine N-acyltransferase, Acyl-CoA:glycine N-acyltransferase, Aralkyl acyl-CoA N-acyltransferase, Aralkyl acyl-CoA:amino acid N-acyltransferase, Benzoyl-coenzyme A:glycine N-acyltransferase, Glycine N-benzoyltransferase, HRP-1(CLP), AAc

2 Images
SDS-PAGE - Recombinant Human GLYAT/GAT protein (AB109928)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human GLYAT/GAT protein (AB109928)

15% SDS-PAGE showing ab109928 at approximately 36.0 kDa (3µg).

SDS-PAGE - Recombinant Human GLYAT/GAT protein (AB109928)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human GLYAT/GAT protein (AB109928)

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q6IB77

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as GLYAT
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMMLPLQGAQMLQMLEKSLRKSLPASLKVYGTVFHINHGNPFNLKAVVDKWPDFNTVVVCPQEQDMTDDLDHYTNTYQIYSKDPQNCQEFLGSPELINWKQHLQIQSSQPSLNEAIQNLAAIKSFKVKQTQRILYMAAETAKELTPFLLKSKILSPSGGKPKAINQEMFKLSSMDVTHAHLVNKFWHFGGNERSQRFIERCIQTFPTCCLLGPEGTPVCWDLMDQTGEMRMAGTLPEYRLHGLVTYVIYSHAQKLGKLGFPVYSHVDYSNEAMQKMSYTLQHVPIPRSWNQWNCVPL","proteinLength":"Full Length","predictedMolecularWeight":"36 kDa","actualMolecularWeight":null,"aminoAcidEnd":296,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q6IB77","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GLYAT also known as glycine-N-acyltransferase or GAT is an enzyme involved in glycine conjugation. This enzyme has a molecular mass of approximately 33 kDa. GLYAT is mainly expressed in the liver and is found in mitochondria. Its mechanical role involves transferring acyl groups from acyl-CoA to glycine forming acyl-glycine conjugates. These conjugates facilitate the excretion of harmful metabolites from the body contributing to detoxification processes.
Biological function summary

GLYAT functions as part of a detoxification pathway. By conjugating glycine to various acyl-CoA substrates it assists in the removal of xenobiotics and other acyl-containing toxins. This action is particularly important for the prevention of acidosis and maintenance of metabolic homeostasis. The enzyme does not typically form part of a larger complex but works alongside other enzymes involved in amino acid metabolism.

Pathways

GLYAT plays an essential role in the glycine conjugation pathway. This pathway is important for detoxifying benzoic acid and other aromatic carboxylic acids. Related proteins include various CoA synthetases which provide the acyl-CoA substrates needed for GLYAT activity. The glycine conjugation pathway intersects with central metabolic networks like amino acid metabolism linking GLYAT functionally to both catabolic and anabolic processes.

Impaired GLYAT function may relate to metabolic issues like hyperglycinemia and certain cases of congenital metabolic disorders of detoxification. Its activity is also relevant in conditions characterized by an accumulation of organic acids. Dysfunctional GLYAT can lead to toxic metabolite build-up emphasizing its importance in maintaining metabolic health. Disorders connected to GLYAT often involve other enzymes in detoxification pathways such as those enzymes implicated in amino acid degradation.
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Specifications

Form

Liquid

Additional notes

ab109928 is purified using conventional chromatography techniques.

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General info

Function

Mitochondrial acyltransferase which transfers an acyl group to the N-terminus of glycine and glutamine, although much less efficiently. Can conjugate numerous substrates to form a variety of N-acylglycines, with a preference for benzoyl-CoA over phenylacetyl-CoA as acyl donors. Thereby detoxify xenobiotics, such as benzoic acid or salicylic acid, and endogenous organic acids, such as isovaleric acid.

Sequence similarities

Belongs to the glycine N-acyltransferase family.

Subcellular localisation

Mitochondrion

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Product protocols

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Target data

Mitochondrial acyltransferase which transfers an acyl group to the N-terminus of glycine and glutamine, although much less efficiently. Can conjugate numerous substrates to form a variety of N-acylglycines, with a preference for benzoyl-CoA over phenylacetyl-CoA as acyl donors. Thereby detoxify xenobiotics, such as benzoic acid or salicylic acid, and endogenous organic acids, such as isovaleric acid.
See full target information GLYAT
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