JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB271532

Recombinant Human Glycogenin 1 + Glycogen synthase 2 protein (Tagged)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human Glycogenin 1 + Glycogen synthase 2 protein (Tagged) is a Human Full Length protein, in the 1 to 350 aa range, expressed in Baculovirus infected Sf9 cells, with >69%, suitable for SDS-PAGE.

View Alternative Names

Glycogen synthase 2, GYS2

1 Images
SDS-PAGE - Recombinant Human Glycogenin 1 + Glycogen synthase 2 protein (Tagged) (AB271532)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Glycogenin 1 + Glycogen synthase 2 protein (Tagged) (AB271532)

SDS-PAGE analysis of 3.6 μg ab271532.

Key facts

Purity

>69% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

Tag free

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P54840

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Preservative: 1.7% Imidazole Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MTDQAFVTLTTNDAYAKGALVLGSSLKQHRTTRRLVVLATPQVSDSMRKVLETVFDEVIMVDVLDSGDSAHLTLMKRPELGVTLTKLHCWSLTQYSKCVFMDADTLVLANIDDLFDREELSAAPDPGWPDCFNSGVFVYQPSVETYNQLLHLASEQGSFDGGDQGILNTFFSSWATTDIRKHLPFIYNLSSISIYSYLPAFKVFGASAKVVHFLGRVKPWNYTYDPKTKSVKSEAHDPNMTHPEFLILWWNIFTTNVLPLLQQFGLVKDTCSYVNVLSDLVYTLAFSCGFCRKEDVSGAISHLSLGEIPAMAQPFVSSEERKERWEQGQADYMGADSFDNIKRKLDTYLQ","proteinLength":"Full Length","predictedMolecularWeight":"42 kDa","actualMolecularWeight":null,"aminoAcidEnd":350,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"P46976","tags":[{"tag":"His","terminus":"N-Terminus"}]},{"sequence":"MLRGRSLSVTSLGGLPQWEVEELPVEELLLFEVAWEVTNKVGGIYTVIQTKAKTTADEWGENYFLIGPYFEHNMKTQVEQCEPVNDAVRRAVDAMNKHGCQVHFGRWLIEGSPYVVLFDIGYSAWNLDRWKGDLWEACSVGIPYHDREANDMLIFGSLTAWFLKEVTDHADGKYVVAQFHEWQAGIGLILSRARKLPIATIFTTHATLLGRYLCAANIDFYNHLDKFNIDKEAGERQIYHRYCMERASVHCAHVFTTVSEITAIEAEHMLKRKPDVVTPNGLNVKKFSAVHEFQNLHAMYKARIQDFVRGHFYGHLDFDLEKTLFLFIAGRYEFSNKGADIFLESLSRLNFLLRMHKSDITVMVFFIMPAKTNNFNVETLKGQAVRKQLWDVAHSVKEKFGKKLYDALLRGEIPDLNDILDRDDLTIMKRAIFSTQRQSLPPVTTHNMIDDSTDPILSTIRRIGLFNNRTDRVKVILHPEFLSSTSPLLPMDYEEFVRGCHLGVFPSYYEPWGYTPAECTVMGIPSVTTNLSGFGCFMQEHVADPTAYGIYIVDRRFRSPDDSCNQLTKFLYGFCKQSRRQRIIQRNRTERLSDLLDWRYLGRYYQHARHLTLSRAFPDKFHVELTSPPTTEGFKYPRPSSVPPSPSGSQASSPQSSDVEDEVEDERYDEEEEAERDRLNIKSPFSLSHVPHGKKKLHGEYKN","proteinLength":"Full Length","predictedMolecularWeight":"82 kDa","actualMolecularWeight":null,"aminoAcidEnd":703,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P54840","tags":[]}]
style
left-column

Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glycogenin 1 also known as glycogen protein or glycogenin protein plays an essential role in the biosynthesis of glycogen. Glycogenin 1 initiates the primer strand for glycogen synthesis by self-glucosylation which is the first step in building a glycogen molecule. Glycogenin 1 typically has a molecular mass of approximately 37 kDa. It expresses primarily in tissues with high energy demands such as liver and skeletal muscle. Glycogen synthase 2 on the other hand is an enzyme involved in elongating the glycogen chain once initiated by Glycogenin 1 working closely together in glycogen metabolism.
Biological function summary

Glycogenin 1 and glycogen synthase 2 play significant roles in glycogen metabolism. Glycogenin 1 starts glycogen synthesis by adding glucose units to itself forming the core structure of glycogen granules. Meanwhile glycogen synthase 2 extends the alpha-14-glucosidic links further enlarging the glycogen molecules. Both are integral to glycogenesis the process of converting glucose into glycogen for storage. These proteins often function in a complex with other enzymes and regulatory proteins ensuring that glycogen synthesis occurs efficiently.

Pathways

Both glycogenin 1 and glycogen synthase 2 are central in glycogen biosynthesis and storage pathways. Glycogen metabolism tightly regulates glucose homeostasis in response to hormonal signals and energy needs. Glycogen phosphorylase an enzyme that breaks down glycogen is influenced by these pathways. Glycogen synthase and glycogenin interact with regulatory kinases and phosphatases which modify their activity in response to signals therefore fine-tuning glycogen levels in cells.

Understanding glycogenin 1 and glycogen synthase 2 interactions has implications in diseases such as Type II diabetes and Glycogen Storage Disease Type 0. Their dysfunction leads to improper glycogen synthesis and disorder in energy balance. For example reduced glycogen synthesis in liver cells can cause fasting hypoglycemia a symptom associated with Glycogen Storage Disease Type 0. The metabolic implications of these proteins link them to pathways with insulin signaling and glucose metabolism impacting proteins like insulin receptor substrate 1 (IRS1) and AMP-activated protein kinase (AMPK) which play roles in maintaining glucose homeostasis.
style
left-column

Specifications

Form

Liquid

Additional notes

Affinity purified.

style
left-column

General info

Function

Glycogen synthase participates in the glycogen biosynthetic process along with glycogenin and glycogen branching enzyme. Extends the primer composed of a few glucose units formed by glycogenin by adding new glucose units to it. In this context, glycogen synthase transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.

Sequence similarities

Belongs to the glycosyltransferase 3 family.

Post-translational modifications

Primed phosphorylation at Ser-657 (site 5) by CSNK2A1 and CSNK2A2 is required for inhibitory phosphorylation at Ser-641 (site 3a), Ser-645 (site 3b), Ser-649 (site 3c) and Ser-653 (site 4) by GSK3A an GSK3B. Dephosphorylation at Ser-641 and Ser-645 by PP1 activates the enzyme (By similarity). Phosphorylation at Ser-8 is not required for interaction with GYG1 (By similarity). Interaction with GYG1 does not regulate the phosphorylation at Ser-8 and Ser-641 (By similarity).

style
left-column

Product protocols

style
left-column

Target data

Glycogen synthase participates in the glycogen biosynthetic process along with glycogenin and glycogen branching enzyme. Extends the primer composed of a few glucose units formed by glycogenin by adding new glucose units to it. In this context, glycogen synthase transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
See full target information GYS2

Additional targets

GYG1
style
left-column
style
left-column
style
right-column

Complementary Products

Proteins & Peptides

AB158615

Recombinant Human Glycogen synthase 1/GYS1 protein

SDS-PAGE - Recombinant Human Glycogen synthase 1/GYS1 protein (AB158615)

  • 0
  • 0
Proteins & Peptides

AB271531

Recombinant Mouse Glycogenin 1 + GYS1 protein (Tagged)

SDS-PAGE - Recombinant Mouse Glycogenin 1 + GYS1 protein (Tagged) (AB271531)

  • 0
  • 0
Primary Antibodies

AB224552

Anti-Glycogen synthase 2 antibody - C-terminal

Western blot - Anti-Glycogen synthase 2 antibody - C-terminal (AB224552)

  • 0
  • 0
Proteins & Peptides

AB162883

Recombinant Human ELOVL2 protein

SDS-PAGE - Recombinant Human ELOVL2 protein (AB162883)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com