Recombinant Human Glypican 3 protein (Tagged) (Biotin) is a Human Fragment protein, in the 25 to 559 aa range, expressed in HEK 293, with >=71% purity and suitable for SDS-PAGE.
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Key facts
- Purity
>=71% SDS-PAGE
- Expression system
HEK 293 cells
- Tags
Avi tag C-Terminus
His tag C-Terminus- Applications
SDS-PAGE
- Biologically active
No
- Conjugation
Biotin
Amino acid sequence
Q P P P P P P D A T C H Q V R S F F Q R L Q P G L K W V P E T P V P G S D L Q V C L P K G P T C C S R K M E E K Y Q L T A R L N M E Q L L Q S A S M E L K F L I I Q N A A V F Q E A F E I V V R H A K N Y T N A M F K N N Y P S L T P Q A F E F V G E F F T D V S L Y I L G S D I N V D D M V N E L F D S L F P V I Y T Q L M N P G L P D S A L D I N E C L R G A R R D L K V F G N F P K L I M T Q V S K S L Q V T R I F L Q A L N L G I E V I N T T D H L K F S K D C G R M L T R M W Y C S Y C Q G L M M V K P C G G Y C N V V M Q G C M A G V V E I D K Y W R E Y I L S L E E L V N G M Y R I Y D M E N V L L G L F S T I H D S I Q Y V Q K N A G K L T T T I G K L C A H S Q Q R Q Y R S A Y Y P E D L F I D K K V L K V A H V E H E E T L S S R R R E L I Q K L K S F I S F Y S A L P G Y I C S H S P V A E N D T L C W N G Q E L V E R Y S Q K A A R N G M K N Q F N L H E L K M K G P E P V V S Q I I D K L K H I N Q L L R T M S M P K G R V L D K N L D E E G F E S G D C G D D E D E C I G G S G D G M I K V K N Q L R F L A E L A Y D L D V D D A P G N S Q Q A T P K D N E I S T F H N L G N V H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cell surface proteoglycan (PubMed:14610063). Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins (By similarity). Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation (By similarity). Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands (PubMed:16227623, PubMed:24496449). Positively regulates the non-canonical Wnt signaling pathway (By similarity). Binds to CD81 which decreases the availability of free CD81 for binding to the transcriptional repressor HHEX, resulting in nuclear translocation of HHEX and transcriptional repression (By similarity). Inhibits the dipeptidyl peptidase activity of DPP4 (PubMed:17549790). Plays a role in limb patterning and skeletal development by controlling the cellular response to BMP4 (By similarity). Modulates the effects of growth factors BMP2, BMP7 and FGF7 on renal branching morphogenesis (By similarity). Required for coronary vascular development (By similarity). Plays a role in regulating cell movements during gastrulation (By similarity).
Alternative names
OCI5, OCI5, GPC3, Glypican-3, GTR2-2, Intestinal protein OCI-5, MXR7
Recommended products
Recombinant Human Glypican 3 protein (Tagged) (Biotin) is a Human Fragment protein, in the 25 to 559 aa range, expressed in HEK 293, with >=71% purity and suitable for SDS-PAGE.
Key facts
- Purity
>=71% SDS-PAGE
- Expression system
HEK 293 cells
- Tags
Avi tag C-Terminus
His tag C-Terminus- Applications
SDS-PAGE
- Biologically active
No
- Conjugation
Biotin
- Accession
- P51654-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.13% Sodium phosphate, 0.02% Potassium chloride
Sequence info
Amino acid sequence
Q P P P P P P D A T C H Q V R S F F Q R L Q P G L K W V P E T P V P G S D L Q V C L P K G P T C C S R K M E E K Y Q L T A R L N M E Q L L Q S A S M E L K F L I I Q N A A V F Q E A F E I V V R H A K N Y T N A M F K N N Y P S L T P Q A F E F V G E F F T D V S L Y I L G S D I N V D D M V N E L F D S L F P V I Y T Q L M N P G L P D S A L D I N E C L R G A R R D L K V F G N F P K L I M T Q V S K S L Q V T R I F L Q A L N L G I E V I N T T D H L K F S K D C G R M L T R M W Y C S Y C Q G L M M V K P C G G Y C N V V M Q G C M A G V V E I D K Y W R E Y I L S L E E L V N G M Y R I Y D M E N V L L G L F S T I H D S I Q Y V Q K N A G K L T T T I G K L C A H S Q Q R Q Y R S A Y Y P E D L F I D K K V L K V A H V E H E E T L S S R R R E L I Q K L K S F I S F Y S A L P G Y I C S H S P V A E N D T L C W N G Q E L V E R Y S Q K A A R N G M K N Q F N L H E L K M K G P E P V V S Q I I D K L K H I N Q L L R T M S M P K G R V L D K N L D E E G F E S G D C G D D E D E C I G G S G D G M I K V K N Q L R F L A E L A Y D L D V D D A P G N S Q Q A T P K D N E I S T F H N L G N V H
- Accession
- Protein length
Fragment
- Predicted molecular weight
63 kDa
- Amino acids
25 to 559
- Nature
Recombinant
- Tags
Avi tag C-Terminus, His tag C-Terminus
Specifications
- Form
Liquid
General info
- Function
Cell surface proteoglycan (PubMed:14610063). Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins (By similarity). Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation (By similarity). Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands (PubMed:16227623, PubMed:24496449). Positively regulates the non-canonical Wnt signaling pathway (By similarity). Binds to CD81 which decreases the availability of free CD81 for binding to the transcriptional repressor HHEX, resulting in nuclear translocation of HHEX and transcriptional repression (By similarity). Inhibits the dipeptidyl peptidase activity of DPP4 (PubMed:17549790). Plays a role in limb patterning and skeletal development by controlling the cellular response to BMP4 (By similarity). Modulates the effects of growth factors BMP2, BMP7 and FGF7 on renal branching morphogenesis (By similarity). Required for coronary vascular development (By similarity). Plays a role in regulating cell movements during gastrulation (By similarity).
- Sequence similarities
Belongs to the glypican family.
- Post-translational modifications
O-glycosylated; contains heparan sulfate and/or chondroitin sulfate.
Storage
- Shipped at conditions
Dry Ice
- Appropriate short-term storage conditions
-80°C
- Appropriate long-term storage conditions
-80°C
- Storage information
Avoid freeze / thaw cycle, Store in the dark
Notes
Enzymatically biotin-labeled using Avi-tag™ technology
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Glypican 3 also known as GPC3 is a cell surface heparan sulfate proteoglycan with a core protein mass of approximately 70 kDa. This target is anchored to the external cell membrane through a glycosylphosphatidylinositol (GPI) anchor. Glypican 3 is expressed mainly in tissues such as the liver and kidneys and plays a role in modulating cell growth and development. In research studies it is often studied in various species including in the mouse and cynomolgus monkey models where it's referred to as mouse glypican 3 and cynomolgus glypican 3 respectively.
- Biological function summary
Glypican 3 is involved in the modulation of signaling pathways that control cell proliferation and differentiation. It does not function as an isolated element; it has a role as part of a complex network that interacts with growth factors morphogens and other proteoglycans. Glypican 3 assists in the regulation of important biological events by influencing the accessibility and activity of signaling molecules on the cell surface. Its heparan sulfate chains interact with multiple proteins modifying their function and availability.
- Pathways
Glypican 3 plays a significant part in the Wnt and Hedgehog signaling pathways. In the Wnt pathway it regulates the distribution and gradient formation of Wnt proteins which are essential for proper embryonic development. In the Hedgehog pathway glypican 3 modulates the distribution and activity of Hedgehog proteins important for tissue patterning and organogenesis. Other proteins closely related to glypican 3 in these pathways include those from the frizzled family in the Wnt pathway and Patched proteins in the Hedgehog pathway.
- Associated diseases and disorders
Glypican 3 has a strong connection to hepatocellular carcinoma (HCC) and Simpson-Golabi-Behmel syndrome (SGBS). In HCC increased expression of glypican 3 is often observed which associates it with tumor growth and proliferation. It functions alongside other oncogenic factors like alpha-fetoprotein (AFP) which is another marker for liver cancer. In SGBS mutations in the GPC3 gene result in defects in cellular signaling pathways leading to developmental abnormalities. This syndrome highlights the impact of glypican 3 on growth regulation and its interactions with insulin-like growth factors (IGFs).
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2 product images
Western blot - Recombinant Human Glypican 3 protein (Tagged) (Biotin) (ab271530)
Recombinant Human Glypican 3 protein (Tagged) (Biotin) (ab271530) used as positive control in WB.
Lane 1: Non-reducing conditions.
Lane 2: Reducing conditions.
All lanes: Western blot - Recombinant Human Glypican 3 protein (Tagged) (Biotin) (ab271530)
SDS-PAGE - Recombinant Human Glypican 3 protein (Tagged) (Biotin) (ab271530)
SDS-PAGE analysis of 7 μg ab271530.
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